The RNomics Platform at Université de Sherbrooke provides a wide range of PCR and bioinformatics services to the research community.
We have developed an automated computational and experimental platform for large scale PCR-based investigations. Primary applications
are for comparative gene expression studies using SYBR green and dual fluorescent probe-based quantitative PCR (qPCR), and the annotation
and analysis of alternative splicing using endpoint PCR coupled with microcapillary electrophoresis (ASPCR). We routinely design, execute and analyze thousands of qPCR and ASPCR reactions per day. Associated services include RNA and DNA isolation and quality control from
cell culture and tissue sources, and extensive bioinformatics support for experimental design and data analysis. Clients can access results and all experimental data via a secure Web interface, where several computational modules are available to optimize data analysis and presentation. The Platform's expert sta is also available for direct consultation at all stages of each project. The Platform is continually
developing new PCR based assays and bioinformatics protocols to meet the needs of its expanding client base.
Applications include:
- Microarray and RNA-Seq validation
- Gene expression analysis
- Alternative splicing annotation
- Custom PCR assays (e.g. telomere length analysis, bacterial strain typing, human DNA quantification)
- Bioinformatics (e.g. functional and sequence data mining, automated PCR primer design, statistical analysis)