The Live Imaging and Bioenergetics Facility at the CUNY ASRC will house three core instruments: 1) Zeiss LSM880 upright two photon confocal microscope with Airyscan, FAST model and CLARITY lens, 2) LSM880 inverted live cell imaging confocal microscope with Airyscan and FAST model, and 3) Agilent Seahorse XFe24 live cell metabolic analyzer.

The supported applications include: in vivo imaging of live animals, time-lapse live cell imaging with high-resolution optical sectioning, deep imaging of fixed CLARITY tissues, calcium imaging, photo switching and photo uncaging, Fluorescence Recovery After Photobleaching (FRAP), Förster Resonance Energy Transfer (FRET), laser ablation, and measuring mitochondrial respiration and glycolysis in live cells in real time. The facility will also provide advanced imaging analysis software Imaris for data processing.

The Epigenetics Core Facility at the Advanced Science Research Center of City University of New York provides a variety of research resources and services that include: nucleic acid extraction and fragmentation, quantitative gene expression analysis, targeted DNA methylation analysis in specific genomic loci of interest, next generation sequencing (RNA-seq and ChIP-seq), and single cell genomics. The facility also houses an advanced cell sorter with biosafety protection for flow cytometry service. In addition, the facility provides services for RNAScope to allow for in situ hybridization detection of transcriptional targets with spatial resolution. The facility is open to both academic and non-academic users.