New York University School of Medicine

Contact Info:

Cytometry and Cell Sorting Laboratory Editor

NYU Langone Medical Center

550 First Avenue

New York, NY 10016

https://med.nyu.edu/research/scientific-cores-shared-resources/precision-immunology-laboratory

Grants and Identifiers:

RRID: RRID:SCR_017936

Instrumentation:

Services Provided:

Cell Imaging

Cell Sorting

Data Analysis

Relevant Publications:

1.) Dioli C, Pappa O, Siatravani E, Bratakou S, Tatsiopoulos A, Giakkoupi P, Miriagou V, Beloukas A (2023 May 26). Molecular Characterization and Prevalence of Antimicrobial-Resistant Escherichia coli Isolates Derived from Clinical Specimens and Environmental Habitats. Microorganisms, 11(6), . . PMID: 37374900.

2.) Azzari C, Cortimiglia M, Nieddu F, Moriondo M, Indolfi G, Mattei R, Zuliani M, Adriani B, Degl'Innocenti R, Consales G, Aquilini D, Bini G, Di Natale ME, Canessa C, Ricci S, de Vitis E, Mangone G, Bechini A, Bonanni P, Pasinato A, Resti M (2016). Pneumococcal serotype distribution in adults with invasive disease and in carrier children in Italy: Should we expect herd protection of adults through infants' vaccination? Human vaccines & immunotherapeutics, 12(2), 344-50. . PMID: 26647277.

3.) Benzer D, Aygun AD, Godekmerdan A, Kurt AN, Akarsu S, Yilmaz E (2006). Serum endothelin-1 and transforming growth factor-beta levels in the newborns with respiratory distress. Mediators of inflammation, 2006(5), 85432. . PMID: 17392579.

Description:

The Precision Immunology Laboratory at NYU Langone studies immune cell phenotype and function, characterizes antitumor immune responses, determines mechanisms of immune escape and evasion, and develops biomarkers that predict patient outcomes or possible toxicities. Offers services for immune monitoring,provides expert knowledge that guides choice of technologies used for study, coordinates purchase, quality control, and use of reagents. Stand-alone services include high-quality cell processing and storage to support correlative studies. These services allow convenient batched analysis of trial samples.Staff purifies peripheral blood mononuclear cells daily from clinic visits and another staff member is available for on-demand and after-hours processing. Samples are cryopreserved on-site in temperature-monitored LN2 freezers for short-term storage. For long-term storage, samples are transferred to Novare Biologistics,off-site cGMP-compliant, New York State-licensed, and FDA-registered biorepository.\\nRoutinely assess quality of processing and cryopreservation. Core meets performance targets for cell viability and recovery necessary for certification by Integrated Biobank of Luxembourg. Offers service for isolating tumor-infiltrating lymphocytes using dissociation protocols optimized for various tumor tissues. Offers flow cytometry at Perlmutter Cancer Center, along with expert service and training. Sterile fluorescence-activated cell sorting (17 colors) is routinely performed with options for high-speed (50K cells per second), single-cell deposition (into polymerase chain reaction plates), and BSL-3 sorting for unscreened or infected human material. Data analysis workstations are also available.Offers single-cell analysis through our cutting-edge 30-parameter flow cytometry technology and Integrated Molecular Cytometry Platforms (IMCPs).