New York University School of Medicine

Contact Info:

Drew Jones

430 E29th Street

WT635

New York, NY 10016

https://med.nyu.edu/research/scientific-cores-shared-resources/metabolomics-laboratory

Grants and Identifiers:

RRID: RRID:SCR_017935

Instrumentation:

Other Equipment

At NYU Langone’s Metabolomics Laboratory, research scientists and bioinformaticians use advanced technologies to perform sophisticated metabolome analyses. We customize our services according to the needs of your research project, whether global (untargeted) or targeted, at competitive rates. Targeted projects are useful for testing a specific hypothesis, often with a large n-value. Global projects are useful for pilot studies or discovery-mode science. The two project types complement each other, with global projects leading to validation by targeted methods. For targeted projects, we quantify the amount of one or more known or predetermined metabolites. For global projects, we detect whatever metabolites are present within a sample or look for differentially regulated metabolites between experimental groups.

Services Provided:

Lipidomics

Mass Spectrometry

Metabolomics

Metabolomics Platform

Untargeted Mass Spectrometry

Relevant Publications:

1.) Zhou C, Pawline MB, Pironti A, Morales SM, Perault AI, Ulrich RJ, Podkowik M, Lejeune A, DuMont A, Stubbe FX, Korman A, Jones DR, Schluter J, Richardson AR, Fey PD, Drlica K, Cadwell K, Torres VJ, Shopsin B (2024 May 11). Microbiota and metabolic adaptation shape Staphylococcus aureus virulence and antimicrobial resistance during intestinal colonization. bioRxiv : the preprint server for biology, (), . . PMID: 38766195.

2.) Buckbinder L, St. Jean DJ Jr, Tieu T, Ladd B, Hilbert B, Wang W, Alltucker JT, Manimala S, Kryukov GV, Brooijmans N, Dowdell G, Jonsson P, Huff M, Guzman-Perez A, Jackson EL, Goncalves MD, Stuart DD (2023 Aug 25). STX-478, a Mutant-Selective, Allosteric PI3Kα Inhibitor Spares Metabolic Dysfunction and Improves Therapeutic Response in PI3Kα-Mutant Xenografts Cancer Discovery, 13(11), 2432-2447. . PMCID: 10618743.

3.) Emrich SM, Yoast RE, Zhang X, Fike AJ, Wang YH, Bricker KN, Tao AY, Xin P, Walter V, Johnson MT, Pathak T, Straub AC, Feske S, Rahman ZSM, Trebak M (2023 Feb 21). Orai3 and Orai1 mediate CRAC channel function and metabolic reprogramming in B cells. eLife, 12(), . . PMID: 36803766.

4.) Oluwadare J, Cabodevilla AG, Son NH, Hu Y, Mullick AE, Verano M, Alemán JO, Ramasamy R, Goldberg IJ (2022 Nov). Blocking Lipid Uptake Pathways Does not Prevent Toxicity in Adipose Triglyceride Lipase (ATGL) Deficiency. Journal of lipid research, 63(11), 100274. . PMID: 36115595.

5.) Guo Y, Xu F, Thomas SC, Zhang Y, Paul B, Sakilam S, Chae S, Li P, Almeter C, Kamer AR, Arora P, Graves DT, Saxena D, Li X (2022 Sep 20). Targeting the succinate receptor effectively inhibits periodontitis. Cell reports, 40(12), 111389. . PMID: 36130514.

6.) Venzon M, Das R, Luciano DJ, Burnett J, Park HS, Devlin JC, Kool ET, Belasco JG, Hubbard EJA, Cadwell K (2022 Jun 8). Microbial byproducts determine reproductive fitness of free-living and parasitic nematodes. Cell host & microbe, 30(6), 786-797.e8. . PMID: 35413267.

Description:

NYU Langone’s Metabolomics Laboratory offers custom metabolomics services to our investigators and the greater scientific community. We specialize in mass spectrometry–based metabolomics, offering global (untargeted) and targeted methods for metabolite identification and quantification. Commonly assayed targets include glycolytic and tricarboxylic acid (TCA) cycle intermediates, amino acids, nucleotides, and lipids. Using a custom-targeted method, we can perform absolute or relative quantification, flux analysis, and biotransformation analysis. Our untargeted platform enables the detection of several thousand metabolite peaks from a typical sample of cells or tissue, focusing on either hydrophilic or hydrophobic metabolites. These data can be used to identify differentially regulated features, candidate markers, metabolite structures, and more. Whether your goal is to measure a specific metabolite or explore the global metabolome of your samples, we tailor the analyses to your experiment.