Iowa State University
MRL Staff
0124 Molecular Biology Building
2437 Pammel Drive
Ames, IA 50011
https://www.biotech.iastate.edu/metabolomics/
RRID: RRID:SCR_017911
(Equipment No Longer Active At this Facility)
Agilent 1260 Infinity II LC System
This HPLC offers choice of modules for analytical HPLC and entry level UHPLC, delivering performance, reliability, and robustness. [Product Link]
RRID:SCR_019355
Agilent 5975C Gas Chromatography Mass Spectrometer
Gas Chromatography Mass Spectrometer is used to meet challenges faced by today's polymer analyst. [Product Link]
RRID:SCR_019415
Agilent 6470 Triple Quadrupole Liquid Chromatography Mass Spectrometer System
Triple quadrupole LC/MS system for commercial, regulatory, life sciences, and research applications. It has Agilent Jet Stream ion source and curved geometry collision cell. It detects target compounds at low levels in diverse matrices, while achieving wide linear dynamic range. [Product Link]
RRID:SCR_019421
(Equipment No Longer Active At this Facility)
Agilent 6545 LC/Q-TOF
LF/Q-TOF is used for untargeted sample analysis and enables broad screening, comprehensive profiling, and identification of unknowns. [Product Link]
RRID:SCR_019429
Agilent 7250 GC/Q-TOF
GC/Q-TOF delivers full spectrum, resolution, and mass data with wide range for identifying and quantifying GC amenable compounds. [Product Link]
RRID:SCR_019441
Agilent 1200 HPLC
Agilent Technologies 1200 Series four solvent gradient capable HPLC coupled to Agilent 1200 series: UV capable diode array detector (DAD), fluorescence detector (FLD), and an evaporative light scattering detector (ELSD). This instrument is equipped with a thermostatically controlled column compartment and auto sampler.
Agilent 6540 LC-QTOF
This mass spectrometer provides ultra-high-definition mass accuracy and mass resolution due to Agilent’s Ion Beam Compression (IBC) and Enhanced Mirror Technology (EMT). Most importantly this Q-TOF possesses the ability to quickly generate accurate mass MS and MS/MS data with UHPLC. This is the perfect system for qualitative analysis in metabolomics profiling studies.
Agilent 7890A GC
Detection and quantification of GC compatible metabolites from various biological samples with a Flame Ionization Detector. APPLICATIONS INCLUDE: High throughput seed fatty acid profile Fatty acid profile from plant or animal tissues, for example muscle and adipose.
(Equipment No Longer Active At this Facility)
Agilent Agilent 6540 LC-Q-TOF
High Definition (UHD) Accurate Mass Q-TOF LC/MS system
BioTek Synergy 2 Multi-Mode Microplate Reader
The Synergy 2 Multi-Mode Microplate Reader has the ability to assay optically active molecules via luminescence, fluorescence, fluorescence polarization and UV-Vis absorbance (monochromator-based).
Amino Acid Analysis
Biochemical Analysis
HPLC
HRAM Mass Spectrometry
Imaging Mass Spectrometry
Lipidomics
Mass Spectrometry
Mass Spectrometry - Small Molecules
Metabolomics
Metabolomics Platform
Molecular Imaging
Untargeted Mass Spectrometry
1.) Chen K, Bhunia RK, Wendt MM, Campidilli G, McNinch C, Hassan A, Li L, Nikolau BJ, Yandeau-Nelson MD (2024 Oct 30). Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment. Journal of experimental botany, 75(20), 6500-6522. . PMID: 39031128.
2.) Weide T, Mills K, Shofner I, Breitzman MW, Kerns K (2024 Jul 19). Metabolic Shift in Porcine Spermatozoa during Sperm Capacitation-Induced Zinc Flux International Journal of Molecular Sciences, 25(14), 7919. . PMCID: 11276750.
3.) Uthaman S, Parvinroo S, Mathew AP, Jia X, Hernandez B, Proctor A, Sajeevan KA, Nenninger A, Long MJ, Park IK, Chowdhury R, Phillips GJ, Wannemuehler MJ, Bardhan R (2024 May 14). Inhibiting the cGAS-STING Pathway in Ulcerative Colitis with Programmable Micelles. ACS nano, 18(19), 12117-12133. doi: 10.1021/acsnano.3c11257. PMID: 38648373.
4.) Meyer C, Grego E, S Vasanthi S, Rao NS, Massey N, Holtkamp C, Huss J, Showman L, Narasimhan B, Thippeswamy T (2023 Nov 30). The NADPH Oxidase Inhibitor, Mitoapocynin, Mitigates DFP-Induced Reactive Astrogliosis in a Rat Model of Organophosphate Neurotoxicity. Antioxidants (Basel, Switzerland), 12(12), . . PMID: 38136181.
5.) Vasanthi SS, Rao NS, Samidurai M, Massey N, Meyer C, Gage M, Kharate M, Almanza A, Wachter L, Mafuta C, Trevino L, Carlo AM, Bryant E, Corson BE, Wohlgemuth M, Ostrander M, Showman L, Wang C, Thippeswamy T (2023 Jul 12). Disease-modifying effects of a glial-targeted inducible nitric oxide synthase inhibitor (1400W) in mixed-sex cohorts of a rat soman (GD) model of epilepsy. Journal of neuroinflammation, 20(1), 163. . ID: 37438764.
6.) Vasanthi SS, Massey N, Nair SN, Mochel JP, Showman L, Thippeswamy T (2023). Exploring the benefits of in-diet versus repeated oral dosing of saracatinib (AZD0530) in chronic studies: insights into pharmacokinetics and animal welfare. Frontiers in veterinary science, 10(), 1297221. . ID: 38026620.
7.) Sirotiak Z, Gallagher BT, Smith-Hernandez CA, Showman LJ, Hillard CJ, Brellenthin AG (2023). Endocannabinoid and psychological responses to acute resistance exercise in trained and untrained adults. PloS one, 18(12), e0291845. . ID: 38039265.
8.) Vinithakumari AA, Padhi P, Hernandez B, Lin SJ, Dunkerson-Kurzhumov A, Showman L, Breitzman M, Stokes C, Sulaiman Y, Tangudu C, Kuttappan DA, Muyyarikkandy MS, Willette AA, Phillips GJ, Anantharam V, Perera A, Sponseller BA, Kanthasamy A, Mooyottu S (2022 Apr 27). Clostridioides difficile Infection Dysregulates Brain Dopamine Metabolism. Microbiology spectrum, 10(2), e0007322. . PMID: 35323033.
9.) Kiefer ZE, Koester LR, Showman L, Studer JM, Chipman AL, Keating AF, Schmitz-Esser S, Ross JW (2021 Mar 17). Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse. Scientific reports, 11(1), 6189. . ID: 33731737.
10.) Aboobucker SI, Showman LJ, Lübberstedt T, Suza WP (2021). Maize Zmcyp710a8 Mutant as a Tool to Decipher the Function of Stigmasterol in Plant Metabolism. Frontiers in plant science, 12(), 732216. . PMID: 34804084.
11.) Campbell AA, Stenback KE, Flyckt K, Hoang T, Perera MAD, Nikolau BJ (2019). A single-cell platform for reconstituting and characterizing fatty acid elongase component enzymes. PloS one, 14(3), e0213620. . ID: 30856216.
12.) Phillips FC, Jensen GS, Showman L, Tonda R, Horst G, Levine R (2019). Particulate and solubilized β-glucan and non-β-glucan fractions of Euglena gracilis induce pro-and anti-inflammatory innate immune cell responses and exhibit antioxidant properties. Journal of inflammation research, 12(), 49-64. . ID: 30881080.
13.) Sharma S, Carlson S, Puttachary S, Sarkar S, Showman L, Putra M, Kanthasamy AG, Thippeswamy T (2018 Feb). Role of the Fyn-PKCδ signaling in SE-induced neuroinflammation and epileptogenesis in experimental models of temporal lobe epilepsy. Neurobiology of disease, 110(), 102-121. . ID: 29197620.
14.) Bhunia RK, Showman LJ, Jose A, Nikolau BJ (2018). Combined use of cutinase and high-resolution mass-spectrometry to query the molecular architecture of cutin. Plant methods, 14(), 117. . PMID: 30603042.
15.) Rizhsky L, Jin H, Shepard MR, Scott HW, Teitgen AM, Perera MA, Mhaske V, Jose A, Zheng X, Crispin M, Wurtele ES, Jones D, Hur M, Góngora-Castillo E, Buell CR, Minto RE, Nikolau BJ (2016 Dec). Integrating metabolomics and transcriptomics data to discover a biocatalyst that can generate the amine precursors for alkamide biosynthesis. The Plant journal : for cell and molecular biology, 88(5), 775-793. . PMID: 27497272.
16.) Bi H, Luang S, Li Y, Bazanova N, Morran S, Song Z, Perera MA, Hrmova M, Borisjuk N, Lopato S (2016 Oct). Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis. Journal of experimental botany, 67(18), 5363-5380. . ID: 27489236.
17.) Wiesen LB, Bender RL, Paradis T, Larson A, Perera MADN, Nikolau BJ, Olszewski NE, Carter CJ (2016 May 2). A Role for GIBBERELLIN 2-OXIDASE6 and Gibberellins in Regulating Nectar Production. Molecular plant, 9(5), 753-756. doi: 10.1016/j.molp.2015.12.019. ID: 26748313.
18.) Noutsos C, Perera AM, Nikolau BJ, Seaver SM, Ware DH (2015). Metabolomic Profiling of the Nectars of Aquilegia pubescens and A. Canadensis. PloS one, 10(5), e0124501. . ID: 25933103.
19.) Noutsos C, Perera AM, Nikolau BJ, Seaver SM, Ware DH (2015). Correction: Metabolomic Profiling of the Nectars of Aquilegia pubescens and A. Canadensis. PloS one, 10(10), e0141384. . ID: 26492042.
20.) Senchina DS, Hallam JE, Kohut ML, Nguyen NA, Perera MA (2014). Alkaloids and athlete immune function: caffeine, theophylline, gingerol, ephedrine, and their congeners. Exercise immunology review, 20(), 68-93. . ID: 24974722.
21.) Bender RL, Fekete ML, Klinkenberg PM, Hampton M, Bauer B, Malecha M, Lindgren K, A Maki J, Perera MA, Nikolau BJ, Carter CJ (2013 Jun). PIN6 is required for nectary auxin response and short stamen development. The Plant journal : for cell and molecular biology, 74(6), 893-904. doi: 10.1111/tpj.12184. ID: 23551385.
22.) Quanbeck SM, Brachova L, Campbell AA, Guan X, Perera A, He K, Rhee SY, Bais P, Dickerson JA, Dixon P, Wohlgemuth G, Fiehn O, Barkan L, Lange I, Lange BM, Lee I, Cortes D, Salazar C, Shuman J, Shulaev V, Huhman DV, Sumner LW, Roth MR, Welti R, Ilarslan H, Wurtele ES, Nikolau BJ (2012). Metabolomics as a Hypothesis-Generating Functional Genomics Tool for the Annotation of Arabidopsis thaliana Genes of "Unknown Function". Frontiers in plant science, 3(), 15. . ID: 22645570.
23.) Muhlemann JK, Maeda H, Chang CY, San Miguel P, Baxter I, Cooper B, Perera MA, Nikolau BJ, Vitek O, Morgan JA, Dudareva N (2012). Developmental changes in the metabolic network of snapdragon flowers. PloS one, 7(7), e40381. . ID: 22808147.
24.) Chen Y, Apolinario E, Brachova L, Kelman Z, Li Z, Nikolau BJ, Showman L, Sowers K, Orban J (2011 Jun 15). A nuclear magnetic resonance based approach to accurate functional annotation of putative enzymes in the methanogen Methanosarcina acetivorans. BMC genomics, 12(), S7. . ID: 21810209.
25.) Senchina DS, Strauch JH, Hoffmann GB, Shah NB, Laflen BK, Dumke BL, Dao CT, Dias AS, Perera MA (2011 Apr). Phytochemical and immunomodulatory properties of an Echinacea laevigata (Asteraceae) tincture. Journal of alternative and complementary medicine (New York, N.Y.), 17(4), 375-7. doi: 10.1089/acm.2010.0373. ID: 21473700.
26.) Perera MA, Qin W, Yandeau-Nelson M, Fan L, Dixon P, Nikolau BJ (2010 Nov). Biological origins of normal-chain hydrocarbons: a pathway model based on cuticular wax analyses of maize silks. The Plant journal : for cell and molecular biology, 64(4), 618-32. doi: 10.1111/j.1365-313X.2010.04355.x. ID: 21070415.
27.) Perera MA, Choi SY, Wurtele ES, Nikolau BJ (2009 Feb 15). Quantitative analysis of short-chain acyl-coenzymeAs in plant tissues by LC-MS-MS electrospray ionization method. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877(5), 482-8. doi: 10.1016/j.jchromb.2008.12.053. PMID: 19157998.
28.) Kram BW, Bainbridge EA, Perera MA, Carter C (2008 Sep). Identification, cloning and characterization of a GDSL lipase secreted into the nectar of Jacaranda mimosifolia. Plant molecular biology, 68(1), 173-83. doi: 10.1007/s11103-008-9361-1. ID: 18553138.
29.) Nikolau BJ, Perera MA, Brachova L, Shanks B (2008 May). Platform biochemicals for a biorenewable chemical industry. The Plant journal : for cell and molecular biology, 54(4), 536-45. doi: 10.1111/j.1365-313X.2008.03484.x. ID: 18476861.
30.) Dietrich CR, Perera MA, D Yandeau-Nelson M, Meeley RB, Nikolau BJ, Schnable PS (2005 Jun). Characterization of two GL8 paralogs reveals that the 3-ketoacyl reductase component of fatty acid elongase is essential for maize (Zea mays L.) development. The Plant journal : for cell and molecular biology, 42(6), 844-61. . ID: 15941398.
31.) Senchina DS, Hallam JE, Dias AS, Perera MA (2009 Nov-Dec). Human blood mononuclear cell in vitro cytokine response before and after two different strenuous exercise bouts in the presence of bloodroot and Echinacea extracts. Blood cells, molecules & diseases, 43(3), 298-303. doi: 10.1016/j.bcmd.2009.08.003. ID: 19766513.
32.) Sebbag L, Showman L, McDowell EM, Perera A, Mochel JP (2018 Jul/Aug). Impact of Flow Rate, Collection Devices, and Extraction Methods on Tear Concentrations Following Oral Administration of Doxycycline in Dogs and Cats. Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 34(6), 452-459. . ID: 29708819.
The W.M. Keck Metabolomics Research Laboratory focuses on GC-MS and LC-MS based non-targeted (total) and custom targeted metabolomic studies as well as high resolution MALDI-MS imaging.
