Northwestern University
Constadina Arvanitis
303 E. Chicago Ave.
Morton Bldg, 2nd floor
Chicago, IL 60611
https://www.feinberg.northwestern.edu/sites/cam/
RRID: RRID:SCR_020996
Baltec Rotary Shadower, Correlative light and electron microscopy, CLEM, Critical point drying, Denton Vacuum Evaporator, Digital and conventional EM image acquisition, Electron microscopy of tissue and cultured cell specimens, Eppendorf FemtoJet injector, FRAP, FRAP-FLIP Calc, FRET, Fluorescence-based minimally invasive small animal intravital imaging, Fluorescence-based non-invasive small animal imaging, , Full service sample preparation and sectioning, HistoQuest, ImageJ, Immunogold EM, platinum replicas Individual training on each instrument, FEI Tecnai Spirit transmission electron microscope, Leica UC6 and UC7 Ultramicrotome, MetaMorph, Microinjection, Narishige Needle Puller, Needle puller, Platinum-replica electron microscopy, Sputter-coating, TissueGnostics Tissue/Cell High Throughput Imaging and Analysis System, TissueQuest, Volocity, Zeiss Axioskop w/ CRi Nuance spectral camera, Nikon A1R confocals, AZ100, SIM, X1 and W1 spinning disks confocal, AiR multiphoton, Biostation CT, Biostation IMQ, Ti2 widefield
Atomic Force Spectroscopy
Cell Imaging
Confocal Microscopy
Data Analysis
Electron Microscopy
Immuno Electron Microscopy
In-vivo Imaging
Laser Capture Microdissection
Light-sheet Microscopy
Live Cell Imaging
Microscopy
Multiphoton Microscopy
Optical Imaging
Outreach
Transmission Electron Microscopy
Whole Slide Imaging
1.) Spurgin S, Nguimtsop AM, Chaudhry FN, Michki SN, Salvador J, Iruela-Arispe ML, Zepp JA, Mukhopadhyay S, Cleaver O (2025 Nov). Dynamics of primary cilia in endothelial and mesenchymal cells throughout mouse lung development. Developmental dynamics : an official publication of the American Association of Anatomists, 254(11), 1187-1202. . PMID: 40055935.
2.) Foo CZ, Duggan A, Bartom ET, Tao L, García-Añoveros J (2025 Oct). Differential Chromatin Accessibility, Gene Expression, and mRNA Splicing Between Developing Cochlear Inner and Outer Hair Cells. Journal of the Association for Research in Otolaryngology : JARO, 26(5), 531-551. . PMID: 40911150.
3.) Hoyt LR, Liu E, Olson EC, Jacobsen DR, Siniscalco ER, Krier-Burris RA, Greenfield KG, McBride CD, Alfajaro MM, Amat JAR, Zhao Z, Xu L, Philip V, Verma A, Fourati S, Senger DL, Zhang L, Bunyavanich S, Glass SE, Coffey RJ, Wilen CB, Williams A, Eisenbarth SC (2025 Aug 7). Cysteinyl leukotrienes stimulate gut absorption of food allergens to promote anaphylaxis in mice. Science (New York, N.Y.), 389(6760), eadp0240. . PMID: 40773557.
4.) Costa RM, Luo JK, Salvino PS, Ackert-Smith LA, Ibarra SM, Pinto L (2025 Jul 24). Cognitive processes are disentangled at cortex-wide scales. bioRxiv : the preprint server for biology, (), . . PMID: 40777310.
5.) Medharametla S, Borger G, Gaonkar S, Valenzuela IMY (2025 Jul 5). Abnormalities in the genioglossus muscle and its neuromuscular synapse in leptin-deficient male mice. Skeletal muscle, 15(1), 18. . PMID: 40616148.
6.) Haynes ME, Arias E, Sullivan DP, Muller WA (2025 Jul 1). PMN recruitment in inflammatory lung injury models follows classical transendothelial migration paradigms requiring PECAM-1 and CD99. American journal of physiology. Lung cellular and molecular physiology, 329(1), L112-L125. . PMID: 40445608.
7.) Clutter ED, Yang W, Han J, Kaplan N, Peng H (2025 Jul 1). Inhibitor of DNA-Binding 3 Is a Novel Regulator of Limbal Epithelial Cell Migration Via the EphA2/Akt Signaling Pathway. Investigative ophthalmology & visual science, 66(9), 2. . PMID: 40590805.
8.) Bresteau E, Suva EE, Revell C, Hassan OA, Grata A, Sheridan J, Mitchell J, Arvanitis C, Korobova F, Woolner S, Jensen OE, Mitchell B (2025 Jun 23). Apical size reduction by macropinocytosis alleviates tissue crowding. Nature communications, 16(1), 5338. . PMID: 40550809.
9.) De Niz M, Pereira SS, Kirchenbuechler D, Lemgruber L, Arvanitis C (2025 Jun 10). Artificial intelligence-powered microscopy: Transforming the landscape of parasitology. Journal of microscopy, (), . doi: 10.1111/jmi.13433. PMID: 40492595.
10.) Liu E, Wright M, Kearney AO, Caza T, Yang JY, Garcia V, Dadi AO, Ishibe S, Chandel NS, Zhang H, Thorp EB, Lin J (2025 Jun 8). G1 and G2 ApolipoproteinL1 modulate macrophage inflammation and lipid accumulation through the polyamine pathway. bioRxiv : the preprint server for biology, (), . . PMID: 40501951.
11.) Converse A, Perry MJ, Dipali SS, Isola JVV, Kelly EB, Varberg JM, Zelinski MB, Stout MB, Pritchard MT, Duncan FE (2025 Jun). Multinucleated giant cells are hallmarks of ovarian aging with unique immune and degradation-associated molecular signatures. PLoS biology, 23(6), e3003204. . PMID: 40550000.
12.) Sadleir KR, Gomez KP, Edwards AE, Patel AJ, Ley ML, Khatri AW, Guo J, Mahesh S, Watkins EA, Popovic J, Karunakaran DKP, Prokopenko D, Tanzi RE, Bustos B, Lubbe SJ, Demonbruen AR, McNally EM, Vassar R (2025 May 24). Annexin A6 membrane repair protein protects against amyloid-induced dystrophic neurites and tau phosphorylation in Alzheimer's disease model mice. Acta neuropathologica, 149(1), 51. . PMID: 40411591.
13.) Piña Novo D, Gao M, Fischer R, Richevaux L, Yu J, Barrett JM, Shepherd GM (2025 May 19). Cortical dynamics in hand/forelimb S1 and M1 evoked by brief photostimulation of the mouse’s hand eLife, 14(), RP105112. . PMCID: 12088680.
14.) Geslewitz WE, Seifert HS (2025 May 12). CRISPRi-mediated repression of three cI repressors induces the expression of three related Neisseria gonorrhoeae bacteriophages Journal of Bacteriology, 207(6), e00049-25. . PMCID: 12186493.
15.) Walters JB, Quezada MJ, He S, Haynes CS, Steece‐Collier K, Collier TJ, Sortwell CE, Franz CK (2025 Apr 21). The Impact of Brain‐Derived Neurotrophic Factor rs6265 (Val66Met) Polymorphism on Therapeutic Electrical Stimulation for Peripheral Nerve Regeneration: A Preclinical Study of Therapy–Genotype Interactions Muscle & Nerve, 72(1), 149-157. . PMCID: 12138492.
16.) Yang Y, Wang TY, Li Q, Lu J, Ren Y, Weiner AB, Fry J, Liu Q, Yum C, Wang R, Guo Q, Wan Y, Ji Z, Dong X, Lotan TL, Schaeffer EM, Yang R, Cao Q (2025 Apr 11). Androgen receptor-regulated lncRNA PRCAT71 promotes AR signaling through the interaction with KHSRP in prostate cancer. Science advances, 11(15), eadk6989. . PMID: 40203114.
17.) Kayaalp Nalbant E, Feliciano TJ, Mohammadlou A, Xiong VL, Trujillo JE, Calvert AE, Kaplan N, Foroozandeh P, Kim J, Bai EM, Qi X, Tobias F, Roth EW, Dravid VP, Lu KQ, Nguyen ST, Shad Thaxton C, Peng H, Lavker RM (2025 Mar 20). A novel therapy to ameliorate nitrogen mustard-induced limbal stem cell deficiency using lipoprotein-like nanoparticles. NPJ Regenerative medicine, 10(1), 14. . PMID: 40113808.
18.) Kirchenbuechler D, De Niz M, Arvanitis C (2025). Trail-blazing and keeping pace: building, retaining and expanding image analysis expertise. Frontiers in bioinformatics, 5(), 1613866. . PMID: 40520511.
19.) Gould A, Luan Y, Hou Y, Korobova FV, Chen L, Arrieta VA, Amidei C, Ward R, Gomez C, Castro B, Habashy K, Zhang D, Youngblood M, Dmello C, Bebawy J, Bouchoux G, Stupp R, Canney M, Yue F, Iruela-Arispe ML, Sonabend AM (2024 Dec 26). Endothelial Response to Blood-Brain Barrier Disruption in the Human Brain. JCI insight, (), . pii: e187328. doi: 10.1172/jci.insight.187328. PMID: 39724015.
20.) Spurgin S, Nguimtsop AM, Chaudhry FN, Michki SN, Salvador J, Iruela-Arispe ML, Zepp JA, Mukhopadhyay S, Cleaver O (2024 Oct 26). Spatiotemporal dynamics of primary and motile cilia throughout lung development. bioRxiv : the preprint server for biology, (), . . PMID: 39484464.
21.) Coy-Dibley J, Jayaraj ND, Ren D, Pacifico P, Belmadani A, Wang YZ, Gebis KK, Savas JN, Paller AS, Miller RJ, Menichella DM (2024 Sep 3). Keratinocyte-Derived Exosomes in Painful Diabetic Neuropathy. bioRxiv : the preprint server for biology, (), . . PMID: 39229068.
22.) Shields MA, Spaulding C, Metropulos AE, Khalafalla MG, Pham TND, Munshi HG (2022 Mar 1). Gα13 loss in Kras/Tp53 mouse model of pancreatic tumorigenesis promotes tumors susceptible to rapamycin. Cell reports, 38(9), 110441. . PMID: 35235808.
23.) Coy-Dibley J, Jayaraj ND, Ren D, Pacifico P, Belmadani A, Wang YZ, Gebis KK, Savas JN, Paller AS, Miller RJ, Menichella DM (2025 Jan-Jun). Keratinocyte-derived extracellular vesicles in painful diabetic neuropathy. Neurobiology of pain (Cambridge, Mass.), 17(), 100176. . PMID: 39811188.
The Center for Advanced Microscopy (CAM) at Northwestern offers state-of-the art instrumentation and services for the study of biological processes at the whole animal, tissue, cellular and subcellular levels. The facility's basic services include electron microscopy, super resolution microscopy (SIM & STORM), fluorescent laser scanning and spinning disk microscopy, fluorescent lifetime imaging, automated high throughput tissue cytometry, atomic force microscopy, laser capture microdissection, mutliphoton imaging, and whole animal bioluminescent and fluorescent imaging. Additionally we provide microinjection equipment, chambers for stable live cell observation, and anesthesia equipment. CAM staff members provide training on numerous different instrument platforms, consultation on experiment design, as well as digital image processing and image analysis. We are a reference site for several companies including TissueGnostics. CAM is one of three Nikon Imaging Centers in the US, allowing us access and excellent support from Nikon to develop innovative solutions for the cutting edge imaging needs of users. This relationship has allowed our users to push super-resolution technologies to permit multi-color, 3D and live-cell applications. Additionally, we have been working together with Nikon to add confocal laser scanning to a low magnification upright dissection microscope, which is particularly useful to researchers interested in visualizing thick samples (such as epidermal raft cultures) at increased cellular resolution. We are also currently expanding our full service electron microscopy offerings. We currently support TEM and SEM sample preparation, immunogold labeling, platinum replicas, and CLEM.
