University of Miami
Yan Shi
1095 NW 14th Terrace
Lois Pope LIFE Center
Miami, FL 33136
http://www.themiamiproject.org/research/core-facilities/high-content-screening/
RRID: RRID:SCR_022542
ThermoFisher Cellomics ArrayScan VTI
PerkinElmer Opera Phenix Plus High-Content Screening System
Assay Development
Assays and Measurements
Cell Imaging
Computer-assisted Neuron Tracing And Analysis
Confocal Microscopy
Confocal/ Super Resolution Microscope
Consultation
Consultations
Data Analysis
Data Analysis And Interpretation
Drug Discovery
Fluorescence Microscope With Optical Sectioning Capability
High Content Screening
Microscopy
Optical Imaging
Pooled Screens
Scanning 20X Brightfield
Scanning 20X Flourescent
Scanning 20X Fluorescent
Scanning 40X Brightfield
Scanning 40X Fluorescent
Small Molecule Compound Screening
1.) Mallela SK, Ge M, Molina J, Santos JV, Kim JJ, Mitrofanova A, Al-Ali H, Marples B, Merscher S, Fornoni A (2022 Feb 3). Sphingomyelin phosphodiesterase acid like 3B (SMPDL3b) regulates Perilipin5 (PLIN5) expression and mediates lipid droplet formation. Genes & diseases, (), . doi: 10.1016/j.gendis.2021.12.014. ID: 35224168.
2.) Ryan CB, Choi JS, Al-Ali H, Lee JK (2022 Feb). Myelin and non-myelin debris contribute to foamy macrophage formation after spinal cord injury. Neurobiology of disease, 163(), 105608. . ID: 34979258.
3.) Khalafi S, Zhu S, Khurana R, Lohse I, Giordano S, Corso S, Al-Ali H, Brothers SP, Wahlestedt C, Schürer S, El-Rifai W (2022 Jan). A novel strategy for combination of clofarabine and pictilisib is synergistic in gastric cancer. Translational oncology, 15(1), 101260. . ID: 34735897.
4.) Lowell JA, O (2021 Dec). Phenotypic Screening Following Transcriptomic Deconvolution to Identify Transcription Factors Mediating Axon Growth Induced by a Kinase Inhibitor. SLAS discovery : advancing life sciences R & D, 26(10), 1337-1354. doi: 10.1177/24725552211026270. ID: 34218704.
5.) Lohse I, Azzam DJ, Al-Ali H, Volmar CH, Brothers SP, Ince TA, Wahlestedt C (2019 Aug). Ovarian Cancer Treatment Stratification Using <i>Ex Vivo</i> Drug Sensitivity Testing. Anticancer research, 39(8), 4023-4030. . ID: 31366484.
6.) Gautam P, Jaiswal A, Aittokallio T, Al-Ali H, Wennerberg K (2019 Jul 18). Phenotypic Screening Combined with Machine Learning for Efficient Identification of Breast Cancer-Selective Therapeutic Targets. Cell chemical biology, 26(7), 970-979.e4. . ID: 31056464.
7.) Mallela SK, Patel DM, Ducasa GM, Merscher S, Fornoni A, Al-Ali H (2019). Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes. Methods in molecular biology (Clifton, N.J.), 1996(), 199-206. doi: 10.1007/978-1-4939-9488-5_17. ID: 31127558.
8.) Al-Ali H, Debevec G, Santos RG, Houghten RA, Davis JC, Nefzi A, Lemmon VP, Bixby JL, Giulianotti MA (2018 Oct 11). Scaffold Ranking and Positional Scanning Identify Novel Neurite Outgrowth Promoters with Nanomolar Potency. ACS medicinal chemistry letters, 9(10), 1057-1062. doi: 10.1021/acsmedchemlett.8b00425. ID: 30344917.
9.) Haggerty AE, Al-Ali H, Oudega M (2018 Sep). Soluble laminin polymers enhance axon growth of primary neurons in vitro. Journal of biomedical materials research. Part A, 106(9), 2372-2381. doi: 10.1002/jbm.a.36429. ID: 29637694.
10.) Lohse I, Al-Ali H, Volmar CH, D Alvarez Trotta A, Brothers SP, Capobianco AJ, Wahlestedt C (2018). Ex vivo drug sensitivity testing as a means for drug repurposing in esophageal adenocarcinoma. PloS one, 13(9), e0203173. doi: 10.1371/journal.pone.0203173. ID: 30212533.
11.) Buchser W, Collins M, Garyantes T, Guha R, Haney S, Lemmon V, Li Z, Trask OJ (2004). Assay Development Guidelines for Image-Based High Content Screening, High Content Analysis and High Content Imaging ID: 23035272.
12.) Al-Ali H, Blackmore M, Bixby JL, Lemmon VP (2004). High Content Screening with Primary Neurons ID: 24260776.
Using the Opera Phenix Plus and a Cellomics ArrayScan VTI and associated automated cellular analysis software, the High Content Screening (HCS) Core provides a cutting edge, multiplexed functional screening and imaging platform to perform multi-well cell-based assays. The core specializes in screening primary neurons, nuclear translocation assays, live dead assays, and lipid droplet assays, amongst others.
