Stanford University
Contact Info:
Andrew Olson
Lorry Lokey Stem Cell Building G0901
265 Campus Drive MC5453
Stanford, CA 94305
Grants and Identifiers:
RRID: RRID:SCR_023257
Instrumentation:
Other Equipment
Confocal microscope (Zeiss LSM510Meta): environmental control for live-cell imaging, spectral unmixing capability, excitation laser lines at 405, 458, 477, 488, 514, 568, 633 nm. Structured illumination super-resolution microscope (GE/Applied Precision OMX V4): SIM excitation laser lines at 405, 488, 561 nm, TIRF imaging at the same wavelengths, fast widefield imaging with solid state illumination 405-633 nm. Two two-photon microscopes (Prairie Technologies): an in vivo rig, and a slice rig; both equipped for 2-photon imaging and simultaneous 2-photon photoactivation. Motorized widefield fluorescence microscope (Zeiss AxioImager): dual CCD cameras: one monochrome, one color; (up to) four-color multi-channel fluorescence with AxioCam HRm monochrome CCD), or histological stains with AxioCam MRc color CCD camera. Two high-powered image analysis workstations: 24 GB RAM, dual quad core processors, high-end video card running Windows 7 64-bit OS; one with Imaris 3D image processing, MatLab, and Fiji; one with NeuroLucida tracing software, MatLab, and Fiji.
Services Provided:
Relevant Publications:
Description:
The Neuroscience Microscopy Service (NMS) provides access to state-of-the-art light microscopy equipment (confocal, super-resolution, two-photon, and widefield microscopes). Image analysis workstations with Imaris, NeuroLucida, MatLab and Fiji software are also available. NMS provides one-on-one training on all equipment.
