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Molecular Genomics Core Facility

Moffitt Cancer Center

Contact Info:

Sean Yoder

13131 Magnolia Dr

Tampa, FL 33612

https://moffitt.org/research-science/shared-resources/molecular-genomics/

Grants and Identifiers:

RRID: RRID:SCR_012333

Instrumentation:

Affymetrix GeneChip 3000 7G Microarray Scanner

Microarray scanner that is an extension of the GeneChip Scanner 3000 (GCS 3000) series that allows you to scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis. [Product Link]

RRID:SCR_019341

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Affymetrix GeneChip 450 Fluidics Station Microarray Processer

The GeneChip Fluidics Station 450 is a microarray processer that washes and stains GeneChip arrays. The GeneChip Fluidics Station 450 is 110V and 220V compatible. [Product Link]

RRID:SCR_018034

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Affymetrix GeneChip Hybridization Oven 645 RNA Analyzer

Hybridization Oven with temperature and rotation control for hybridization of as many as 64 GeneChip cartridges at one time. The oven can be configured for 110 or 220V operation. [Product Link]

RRID:SCR_019345

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Illumina MiSeq System

Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]

RRID:SCR_020134

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Illumina NextSeq 550 System

With tunable output and high data quality, it provides flexible power you need for whole genome, transcriptome, and targeted resequencing plus ability to scan microarrays including Infinium MethylationEPIC BeadChip and other select BeadChips. [Product Link]

RRID:SCR_020141

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Illumina NovaSeq 6000 Sequencing System

Unleashes groundbreaking innovations that leverage our technology Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. [Product Link]

RRID:SCR_020150

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10X Genomics Chromium X Controller

Single-cell RNA and ATAC-sequncing

MissionBio Tapestri v2

Single-cell DNA Sequencing Platform

NanoString nCounter Digital Adnalzyer

Cartridge imager

NanoString Technologies nCounter Prep Station

Automation Instrumentation

Other Equipment

Equipment in the Core Illumina HiScan SQ - Illumina Sequencing and BeadArrays NanoString nCounter Analysis System Affymetrix Fludics Stations and Scanners x 4 Illumina BeadXpress Agilent Microarray Scanner Tecan Slide Hybridization and Wash System ABI 7900HT Real-Time PCR System x 2 ABI 3130xl Genetic Analyzer Capillary Sequencer

Services Provided:

10x Genomics

ATAC

ATAC-Seq

ChIP-Seq

CITE-seq

Copy Number Variation (CNV)

Custom Library Prep Service

DNA Analysis

FFPE Restore

Gene Expression Arrays

Genomics

Genotyping

Genotyping-by-sequencing

Metagenomics Analysis

Microarray

Microbiome

Nanostring

Nanostring NCounter

RNA analysis

RNA Integrity

RNAseq

Sequencing - DNA Sequencing

Sequencing - Next Generation Sequencing (NGS)

Single-Cell Analysis

Single-cell Sequencing

Whole Exome Sequencing

Whole Genome Sequencing

Relevant Publications:

Description:

The Molecular Genomics Core Facility at Moffitt Cancer Center in Tampa, FL offers services to assess gene expression, epigenetic modification and sequence variation. We perform bulk and single-cell RNA and DNA sequencing on the Illumina platform, NanoString nCounter, GeneChip and Illumina array services. Please contact us to discuss your project. If it is something we can help you with, we can quickly provide a quote and a timeline for your project.