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Advanced Genome Technologies Core Massively Parallel Sequencing Facility

University of Vermont

Contact Info:

Jessica Hoffman

149 Beaumont Avenue

305 HSRF

Burlington, VT 05405

https://www.med.uvm.edu/vigr/mpsf

Grants and Identifiers:

RRID: RRID:SCR_022696

Instrumentation:

Other Equipment

Illumina HiSeq 1500 Covaris S220 AFA Nanopore Sequencer GridION X5 Minion MK1C Minion MK1B MiniSeq Miseq ddPCR-BioRad QX200 Quantstudio 6F Bioanlayzers

Services Provided:

10x Genomics

ATAC

ATAC-Seq

Capillary Electrophoresis

CDNA Synthesis

Cell Line Authentication

Cell Lines For SARS-CoV-2 Research

ChIP-Seq

CITE-seq

Consultation

Copy Number Variation (CNV)

Data Analysis

Digital PCR

Droplet Digital PCR

Genomics

Genotyping

Long Read Sequencing

Metabtranscriptomic

Metagenomics Analysis

Metatranscriptomics

Microbiological Culturing

Microbiome

Nanopore Sequencing

Nucleic Acid Extraction

Protocol Development

Proximity-Ligation

Real-time qPCR

Ribosomal Reduction

RNA analysis

RNA Integrity

RNA Isolation

RNA Modification Analysis

RNAseq

SARS-CoV-2 Strain Sequencing

SARS-CoV-2 Wastewater Testing

Sequencing - Next Generation Sequencing (NGS)

Single-Cell Analysis

Single-cell Sequencing

Spatial Transcriptomics

TaqMan Arrays

Tissue Extraction

Whole Genome Amplification (WGA)

Whole Genome Sequencing

Relevant Publications:

Description:

This facility is centrally located on the third floor of the Health Science Research Facility and includes infrastructure that provides comprehensive support for the design, execution, and analysis of experiments involving Next Generation Sequencing through access and use of the Illumina HiSeq 1500 sequencer. All next generation sequencing projects are reviewed using an integrated approach to experimental design, workflow, and sample collection in collaboration with the UVM bioinformatics core facilities to ensure high quality results. The facility is directed by Julie Dragon and Scott Tighe, and performs a wide range of analysis including target preparation for RNAseq, Exome Seq, ChIP seq, Methyl Seq, whole genome sequencing, and small RNA sequencing.The core is heavily involved in all aspects of Nanopore sequencing