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CRISPR and Genome Editing Resource Center

Rockefeller University

Contact Info:

Chingwen Yang

1230 York Avenue

Box 2

New York, NY 10065

https://www.rockefeller.edu/crispr/

Grants and Identifiers:

RRID: RRID:SCR_023097

Instrumentation:

Services Provided:

CRISPR/Cas Project Design

Genotyping

Relevant Publications:

Description:

The CRISPR and Genome Editing Resource Center provides gene targeting and mouse embryonic stem cell technology for members of the Rockefeller University, Memorial Sloan-Kettering Cancer Center and their collaborators. Since its inception in 1997 our center has generated numerous genetically engineered mice and embryonic stem cell lines that allow the investigation of gene functions in mouse models or ES cell culture systems. We continue developing and adopting innovative approaches to better assist biomedical research in our community. CRISPR and Genome Editing services include mouse genomic knock-out and knock-in, mouse conditional knock-out, establishing new ES lines for gene targeting services, establishing ES lines from mutant mouse embryos, preparing ES cells introduced from off campus resources for injection or other application, assisting in embryoid body culture and in vitro differentiation of ES cells, and purification and evaluation of DNA for pronuclear injection.