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Laboratorio Nacional de Microscopia Avanzada-IMSS (LNMA-IMSS)

Contact Info:

Vadim Perez Koldenkova

Centro Médico Nacional Siglo XXI, IMSS. Av. Cuauhtémoc, 330.

Col. Doctores, Alc. Cuauhtémoc, CP06720

Mexico City

https://imss.lnma.mx/inicio.php

Grants and Identifiers:

RRID: RRID:SCR_027351

Instrumentation:

Nikon A1 Confocal Laser Microscope

Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]

RRID:SCR_020318

cite this instrument

(Equipment No Longer Active At this Facility)

KFBIO KF-FL-400

Fluorescence and brightfield slide scanner

KFBIO KF-FL-400

Fluorescence and brightfield slide scanner

Nikon AZ100 Macroscope

Macroscope

Services Provided:

Fluorescence Microscope With Optical Sectioning Capability

Optical Imaging

Relevant Publications:

1.) De Leon-Rodríguez SG, Aguilar-Flores C, Gajón JA, Mantilla A, Gerson-Cwilich R, Martínez-Herrera JF, Rodríguez-Soto BE, Gutiérrez-Quiroz CT, Pérez-Koldenkova V, Muñoz-Cruz S, Bonifaz LC, Fuentes-Pananá EM (2023 Mar 1). Acral Melanoma Is Infiltrated with cDC1s and Functional Exhausted CD8 T Cells Similar to the Cutaneous Melanoma of Sun-Exposed Skin. International journal of molecular sciences, 24(5), . . ID: 36902214.


2.) De León Rodríguez SG, Hernández Herrera P, Aguilar Flores C, Pérez Koldenkova V, Guerrero A, Mantilla A, Fuentes-Pananá EM, Wood C, Bonifaz LC (2022). A Machine Learning Workflow of Multiplexed Immunofluorescence Images to Interrogate Activator and Tolerogenic Profiles of Conventional Type 1 Dendritic Cells Infiltrating Melanomas of Disease-Free and Metastatic Patients. Journal of oncology, 2022(), 9775736. . ID: 36276271.


Description:

Optical microscopy and histology services core facility.