Beth Israel Deaconess Medical Center
Chi Ngai Chan
3 Blackfan Circle E/CLS-1032
BIDMC
Boston, MA 02115
https://cvvr.hms.harvard.edu/tissue-technologies-unit/#overview
RRID: RRID:SCR_027102
10x Genomics Visium
Akoya PhenoCycler Fusion
CODEX
Cryostat Sectioning
Histology Stains
Immunohistochemistry
Multiplex Immunofluorescence
Nanostring CosMX
Processing & Embedding- Histology
RNAscope
Spatial Transcriptomics
Tissue Clearing
Tissue MicroArray
Tissue Processing
Tissue Sectioning
1.) Gort-Freitas NA, Moscvin M, Da Viá MC, Lazzaroni F, Nevone A, Sadigh S, Boullt S, Evans B, Chen T, Karagiannis TT, Tai A, Rowell S, Raghav S, Chen AF, Laubach JP, Edwards C, Aster JC, Sheng Z, Paulo JA, Chan CN, Nuvolone M, Bolli N, Comenzo RL, Klein A, Bianchi G (2025 Jun 10). Single-cell and clonal analysis of AL amyloidosis plasma cells and their bone marrow microenvironment. Blood, (), . pii: blood.2024024719. doi: 10.1182/blood.2024024719. ID: 40493887.
2.) Pereira Ribeiro S, Strongin Z, Soudeyns H, Ten-Caten F, Ghneim K, Pacheco Sanchez G, Xavier de Medeiros G, Del Rio Estrada PM, Pelletier AN, Hoang T, Nguyen K, Harper J, Jean S, Wallace C, Balderas R, Lifson JD, Raghunathan G, Rimmer E, Pastuskovas CV, Wu G, Micci L, Ribeiro RM, Chan CN, Estes JD, Silvestri G, Gorman DM, Howell BJ, Hazuda DJ, Paiardini M, Sekaly RP (2025 Mar). Author Correction: Dual blockade of IL-10 and PD-1 leads to control of SIV viral rebound following analytical treatment interruption. Nature immunology, 26(3), 524. . ID: 39821151.
3.) Yeo YY, Chang Y, Qiu H, Yiu SPT, Michel HA, Wu W, Jin X, Kure S, Parmelee L, Luo S, Cramer P, Lee JL, Wang Y, Yeung J, Ahmar NE, Simsek B, Mohanna R, Van Orden M, Lu W, Livak KJ, Li S, Shahryari J, Kingsley L, Al-Humadi RN, Nasr S, Nkosi D, Sadigh S, Rock P, Frauenfeld L, Kaufmann L, Zhu B, Basak A, Dhanikonda N, Chan CN, Krull J, Cho YW, Chen CY, Lee JYJ, Wang H, Zhao B, Loo LH, Kim DM, Boussiotis V, Zhang B, Shalek AK, Howitt B, Signoretti S, Schürch CM, Hodi FS, Burack WR, Rodig SJ, Ma Q, Jia (2024 Dec 22). Same-Slide Spatial Multi-Omics Integration Reveals Tumor Virus-Linked Spatial Reorganization of the Tumor Microenvironment. bioRxiv : the preprint server for biology, (), . . ID: 39764057.
4.) Pereira Ribeiro S, Strongin Z, Soudeyns H, Ten-Caten F, Ghneim K, Pacheco Sanchez G, Xavier de Medeiros G, Del Rio Estrada PM, Pelletier AN, Hoang T, Nguyen K, Harper J, Jean S, Wallace C, Balderas R, Lifson JD, Raghunathan G, Rimmer E, Pastuskovas CV, Wu G, Micci L, Ribeiro RM, Chan CN, Estes JD, Silvestri G, Gorman DM, Howell BJ, Hazuda DJ, Paiardini M, Sekaly RP (2024 Oct). Dual blockade of IL-10 and PD-1 leads to control of SIV viral rebound following analytical treatment interruption. Nature immunology, 25(10), 1900-1912. . ID: 39266691.
5.) Bhattacharya A, Wang K, Penailillo J, Chan CN, Fushimi A, Yamashita N, Daimon T, Haratake N, Ozawa H, Nakashoji A, Shigeta K, Morimoto Y, Miyo M, Kufe DW (2024 Jul). MUC1-C regulates NEAT1 lncRNA expression and paraspeckle formation in cancer progression. Oncogene, 43(28), 2199-2214. . ID: 38802648.
6.) Ryan KS, Karpf JA, Chan CN, Hagen OL, McFarland TJ, Urian JW, Wang X, Boniface ER, Hakar MH, Terrobias JJD, Graham JA, Passmore S, Grant KA, Sullivan EL, Grafe MR, Saugstad JA, Kroenke CD, Lo JO (2024 Mar 9). Prenatal delta-9-tetrahydrocannabinol exposure alters fetal neurodevelopment in rhesus macaques. Scientific reports, 14(1), 5808. . ID: 38461359.
7.) Sureshchandra S, Chan CN, Robino JJ, Parmelee LK, Nash MJ, Wesolowski SR, Pietras EM, Friedman JE, Takahashi D, Shen W, Jiang X, Hennebold JD, Goldman D, Packwood W, Lindner JR, Roberts CT Jr, Burwitz BJ, Messaoudi I, Varlamov O (2022 Dec 13). Maternal Western-style diet remodels the transcriptional landscape of fetal hematopoietic stem and progenitor cells in rhesus macaques. Stem cell reports, 17(12), 2595-2609. . ID: 36332628.
8.) Sinnamon JR, Jacobson ME, Yung JF, Fisk JR, Jeng S, McWeeney SK, Parmelee LK, Chan CN, Yee SP, Mandel G (2022 Aug 16). Targeted RNA editing in brainstem alleviates respiratory dysfunction in a mouse model of Rett syndrome. Proceedings of the National Academy of Sciences of the United States of America, 119(33), e2206053119. . ID: 35939700.
9.) Jiang S, Chan CN, Rovira-Clavé X, Chen H, Bai Y, Zhu B, McCaffrey E, Greenwald NF, Liu C, Barlow GL, Weirather JL, Oliveria JP, Nakayama T, Lee IT, Matter MS, Carlisle AE, Philips D, Vazquez G, Mukherjee N, Busman-Sahay K, Nekorchuk M, Terry M, Younger S, Bosse M, Demeter J, Rodig SJ, Tzankov A, Goltsev Y, McIlwain DR, Angelo M, Estes JD, Nolan GP (2022 Jun 14). Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironments. Immunity, 55(6), 1118-1134.e8. . ID: 35447093.
10.) Aid M, Vidal SJ, Piedra-Mora C, Ducat S, Chan CN, Bondoc S, Colarusso A, Starke CE, Nekorchuk M, Busman-Sahay K, Estes JD, Martinot AJ, Barouch DH (2022 Apr). Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques. PLoS pathogens, 18(4), e1009990. . ID: 35395058.
The Tissue Technologies Unit provides research-focus histology, Immunohistochemistry, In-situ Hybridization (ACDBio RNAscope and Basescope) and Highplex Spatial-omics data generation (Akoya CODEX Fusion, 10X Genomics Visium HD, NanoString GeoMX and CosMX). Our other unique capabilities include BSL-2 NHP tissue processing and cryosectioning, tissue microarray (TMA) construction and multiplexing RNAScope and Protein marker staining (PANINI).
