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NCSU Genomic Sciences Laboratory

North Carolina State University

Contact Info:

Kelly Sides

112 Derieux Place

Thomas Hall Room 2518

Raleigh, NC 27606

https://research.ncsu.edu/gsl/

Grants and Identifiers:

RRID: RRID:SCR_026944

Instrumentation:

ABI 3700 Genetic Analyzer

Automated capillary gel electrophoresis genetic analyzer that sequences up to 96 DNA templates per run and is used for PCR sequencing and short tandem repeat genotyping. [Product Link]

RRID:SCR_018431

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Eppendorf epMotion 5075t NGS Solution Automated Pipetting

Automated pipetting workstation that combines automated liquid handling and software features with accessories and consumables for Next Generation Sequencing library preparation and NGS kit flexibility. [Product Link]

RRID:SCR_019867

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Illumina MiSeq System

Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]

RRID:SCR_020134

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Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems

Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]

RRID:SCR_020140

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Illumina NovaSeq 6000 Sequencing System

Unleashes groundbreaking innovations that leverage our technology Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. [Product Link]

RRID:SCR_020150

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PacBio Sequel IIe

Long Read Sequencer

Services Provided:

16S Amplicon Sequencing

ABI3730XL Sanger Sequencing And Fragment Analysis

BluePippin Automated Size Selection

DNA Isolation

Illumina MiSeq Sequencing

Illumina NextSeq Sequencing

Illumina RNA/DNA NGS Library Construction

RNA Isolation

RNA/DNA Quality Control

Single Cell RNAseq

Relevant Publications:

Description:

Established in 2000, the mission of the Genomic Sciences Laboratory (GSL) is to enable discovery in the biological sciences by providing researchers and students with the necessary cutting-edge equipment and infrastructure required to conduct advanced genomics research. The GSL operates as a solution center and provides NC State researchers, collaborators, and the broader scientific community with access to high-throughput DNA sequencing, traditional Sanger sequencing and genotyping, and additional equipment services for functional genomic assays and mapping. The GSL also provides high-throughput robotics and instrumentation to facilitate the handling of large numbers of samples, as well as many advanced sequencing and sequencing support applications developed independently by NC State faculty. The GSL is administered by the Office of Research and Innovation at NC State and operates on a fee-recovery basis.