Rush University Medical Center
Contact Info:
Kevin Kunstman
1750 West Harrison
Jelke 444
Chicago, IL 60612
Grants and Identifiers:
RRID: RRID:SCR_026856
Instrumentation:
Agilent 4150 Tapestation Genetic Analyzer
Agilent 4150 TapeStation system analyzes nucleic acids for up to 16 samples per run. [Product Link]
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Illumina MiniSeq Next Generation Sequencer
Illumina NGS technology performs broad range of targeted DNA and RNA applications. [Product Link]
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(Equipment No Longer Active At this Facility)
Illumina MiSeq System
Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]
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Maxwell 16 DNA purification system
Nucleic Acid and Protein Purification instrument that provides consistent, automated purification of high-quality DNA, RNA, viral total nucleic acid, or recombinant proteins using paramagnetic particles. There are no Clogs or Drips, Optimal Capture, Washing, and Elution of Target Material, Purifier Can Configure as an SEV or LEV Instrument, Preprogrammed Purification Protocols, For Purification of High-Quality DNA, RNA, and More. It works as a particle processor, not a liquid handler, so there are no clogs or drips, allowing optimal capture, washing, and elution of target material. Purification protocols are preprogrammed into the instrument and can be combined with purification kits containing prefilled reagent cartridges to maximize simplicity and convenience. The purifier can be configured as an SEV Instrument (Standard Elution Volume, 200 400 ?L) for maximum yield or an LEV Instrument (Low Elution Volume, 30 100 ?L) for maximum concentration. Accessories information: Kits are sold separately and contain enough materials to run 48 preps each. [Product Link]
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Promega GloMax 96 Microplate Luminometer
Microplate luminometer that has injectors and reads both glow and flash luminescent reactions in 96 well plates. It can be used for luminescent assays, Promega bioluminescent reporter, cell based and biochemical assays. [Product Link]
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Qiagen QIAcube
DNA/RNA Protein Purification System for automated purification of nucleic acids and proteins in molecular biology applications. [Product Link]
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Services Provided:
Relevant Publications:
Description:
The GMCF is a full-service Genomics and Next Generation Sequencing (NGS) facility catering to internal and external investigators across a wide range of biological systems. The GMCF has unique expertise in microbial/microbiome sequencing, but also has extensive capabilities for nucleic acid extraction, bulk NGS library prep (DNA and RNAseq), single cell sequencing (10X and Fluent), spatial sequencing (10X Visium), methyl-seq, NGS amplicon sequencing (any target), and quantitative analyses (sequencing, real-time PCR, digital PCR).
