Northwestern University
Contact Info:
Clark Rosensweig
2205 Tech Drive
Evanston, IL 60208
https://sites.northwestern.edu/singlecellgenomics/
Grants and Identifiers:
RRID: RRID:SCR_026652
Instrumentation:
BD FACSDiscover S8
Cell Sorter, equipped with 5 lasers (355, 405, 488, 561 and 640 nm excitation wavelengths) and 78 channels which can measure the entire emission spectra of the fluorescent dyes, is the first spectral flow cytometer sorter with sort-capable image analysis. [Product Link]
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BD LSRFortessa Cell Analyzer
BD LSRFortessa system can be configured with up to 7 lasers, blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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Life Technologies QuantStudio 3 Real Time PCR System
Applied Biosystems QuantStudio 3 Real-Time PCR System is designed for users who need an , easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is best for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 3 system provides access to your data wherever and whenever you want. Using OptiFlex technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex temperature zones, the QuantStudio 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications. [Product Link]
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Zeiss Axio Vert series Axiovert 200 inverted microscope
Inverted microscope that is used for the examination of cell and tissue culture of sediments in culture flasks, Petri dishes, microtiter plates, etc. in transmitted and reflected light. It hass attachment possibilities for various tools (micromanipulation), different light sources, temperature control devices. [Product Link]
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Services Provided:
Bulk RNA Library Prep Services
Low Input/Single Cell Library Prep Services
Nucleic Acid Quantitation (TapeStation, Qubit)
Single Nucleus Dissociation (Frozen)
Relevant Publications:
Description:
A Northwestern University core facility dedicated to supporting single cell and spatial biology experiments on the Evanston campus. Also the home of flow cytometry operations for the Evanston campus.
