Beth Israel Deaconess Medical Center
Hagen, Susan J
330 Brookline Ave # DA-814
Boston, MA 02215-5400
https://research.bidmc.org/confocal-imaging-core
RRID: RRID:SCR_012312
Zeiss LSM 880 Live Cell Confocal System with Fast Airyscan
Zeiss LSM880 Upright Platform Confocal System
Biological Photography/Photomicrography
Confocal Microscopy
Fluorescence TUNEL Staining-Paraffin Sections
Fluorescence-multi-label Using Paraffin Sections
Fluorescence-single Label Using Paraffin Sections
Immunoperoxidase (IPOX) Using Frozen Sections
IPOX-Paraffin Sections
Labeling Of Frozen Sections Using A Single Fluorescence Label
Labeling Of Frozen Sections Using Multiple Fluorescence Labels;
Near Super-resolution Confocal Microscopy
Pre-antibody Testing/optimization With New Antibodies.
Pre-experiment Consultation And Consultation Prior To Antibody Purchase
Widefield Microscopy
1.) Chen AM, Azar SS, Harris A, Brecha NC, Pérez de Sevilla Müller L (2021). PTEN Expression Regulates Gap Junction Connectivity in the Retina. Frontiers in neuroanatomy, 15(), 629244. . PMID: 34093139.
2.) Pérez de Sevilla Müller L, Solomon A, Sheets K, Hapukino H, Rodriguez AR, Brecha NC (2019 Jan 1). Multiple cell types form the VIP amacrine cell population. The Journal of comparative neurology, 527(1), 133-158. doi: 10.1002/cne.24234. PMID: 28472856.
3.) Solomon AM, Westbrook T, Field GD, McGee AW (2018). Nogo receptor 1 is expressed by nearly all retinal ganglion cells. PloS one, 13(5), e0196565. . PMID: 29768445.
4.) Pérez de Sevilla Müller L, Azar SS, de Los Santos J, Brecha NC (2017). Prox1 Is a Marker for AII Amacrine Cells in the Mouse Retina. Frontiers in neuroanatomy, 11(), 39. . PMID: 28529477.
5.) Vujovic N, Gooley JJ, Jhou TC, Saper CB (2015 Dec 15). Projections from the subparaventricular zone define four channels of output from the circadian timing system. The Journal of comparative neurology, 523(18), 2714-37. . PMID: 26010698.
6.) Pérez de Sevilla Müller L, Sargoy A, Fernández-Sánchez L, Rodriguez A, Liu J, Cuenca N, Brecha N (2015 Jul 1). Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina. The Journal of comparative neurology, 523(10), 1443-60. . PMID: 25631988.
7.) Liu X, Hein J, Richardson SC, Basse PH, Toptan T, Moore PS, Gjoerup OV, Chang Y (2011 May 13). Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus. The Journal of biological chemistry, 286(19), 17079-90. . PMID: 21454559.
The BIDMC Immunostaining and Microscopy Core offers consultation and service for tissue immunostaining with paraffin or frozen sections, and for training to use confocal and widefield microscopes. All service work and training is fee-for-service.
