Beth Israel Deaconess Medical Center

Contact Info:

Hagen, Susan J

330 Brookline Ave # GZB05

Boston, MA 02215-5400

https://research.bidmc.org/em-core

Grants and Identifiers:

RRID: RRID:SCR_009667

Instrumentation:

Services Provided:

Contrast Staining

Discussion Of Results (consultation) And Transfer Of Image Data Files (no Storage)

Evaluation Of The Sample Using The JEOL 1400 Electron Microscope

High Pressure Freezing

Image Analysis

Negative Staining For TEM

Pre-experiment Consultation For Methods And For Proper Fixation Techniques

Receipt Of Samples (sometimes Assisting With Sample Collection) Followed By Grossing And Processing Of Tissues, Suspended Cells, Bacteria, Cultured Cells, Organoids, Exosomes

Review Of Sections For Tissue Orientation And Histopathology

Thin Sectioning, Production And Use Of Formvar- And Carbon-coated Grids

Relevant Publications:

Description:

The BIDMC Electron Microscopy Core provides service for grossing, tissue processing, tissue embedding, thick and thin sectioning, staining grids, and taking images of samples in the electron microscope. Training is available to use the electron microscope independently. For tissue processing, we also have training or service for high pressure freezing, freeze substitution, and tissue embedding. The core also has the expertise to do Advanced 3-D Focused Ion Beam/Scanning Electron Microscopy and 3-D segmentation and analysis on cells or tissues.