University of British Columbia
Contact Info:
Lisa Xu
938 W 28th Avenue
A4-143 TRB BCCHR
Vancouver, BC V5Z 4H4
Grants and Identifiers:
RRID: RRID:SCR_026601
Instrumentation:
BD LSRFortessa X-20 Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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Beckman Coulter CytoFLEX Flow Cytometer
Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]
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Services Provided:
Relevant Publications:
Description:
Flow cytometry is a powerful technology used for characterizing and analyzing cells. The fluidics, optics, and electronics systems in flow cytometry work together to simultaneously measure and analyze multiple physical characteristics of particles, usually, cells, as they move in a fluid stream through a beam of light. A flow cytometer can tell us about a particle’s relative size, fluorescence intensity, and granularity or internal complexity. The BCCHR Flow Core Facility provides equipment for flow cytometric analysis and full-service cell sorting, as well as flow cytometry training and education for new users. This facility operates on a fee-for-service basis, which allows for partial cost recovery for operating expenses and service contracts for the instruments. A portion of the facility operating and instrumentation costs are provided through financial support from the BC Children's Hospital Research Institute and Foundation, University of British Columbia and Canada Foundation for Innovation (CFI) awards to contributing investigators.
