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Viničná Microscopy Core Facility, Faculty of Science

Charles University

Contact Info:

Martin Schätz

Viničná 7

Prague 128 00

https://web.natur.cuni.cz/sekce-bi/VMCF/

Grants and Identifiers:

RRID: RRID:SCR_026602

Other Citation Identifiers

Czech-BioImaging large RI project LM2023050

e-INFRA CZ project (ID:90254)

Instrumentation:

Flow Cytometer BC Cytoflex

Flow Cytometer BD LSR II

Flow Sorter Sony MA900

JEOL High Resolution SEM JSM-IT800

JEOL Scanning Electron Microscope JSM-6380LV

JEOL Transmission Electron Microscope JEM-1011

(Equipment No Longer Active At this Facility)

Leica Confocal Microscope Leica SP8

Leica Confocal Microscope SP8

Nikon Wide-field microscope TiE2

Olympus Wide-field Microscope Cell^R

Zeiss Confocal Microscope LSM880 NLO

Zeiss Elyra SP1

Zeiss Lightsheet Z1

Zeiss Slide Scanner Axioscan Z1

Zeiss Stereomicroscope Axiozoom

Services Provided:

3D Printing

Bioimage Informatics

Brightfield Microscopy

Confocal Microscope With FCS/FLIM Capabilities

Confocal Microscopy

Consultation For Pre-experiment Methods, Post-processing Orientation, And/or Special Staining

Electron Microscopy

Experimental Design

Flow Cytometry

Image Processing And Analysis

Lightsheet

Super-resolution Microscopy

Relevant Publications:

1.) Bogusch P, Petřík O, Hlaváček A, Šebesta O, Šípek P (2025 Jan 10). Thermal Melanism in Pachnoda iskuulka (Coleoptera: Scarabaeidae: Cetoniinae). Insects, 16(1), . . ID: 39859642.


2.) Hovorková M, Kaščáková B, Petrásková L, Havlíčková P, Nováček J, Pinkas D, Gardian Z, Křen V, Bojarová P, Smatanová IK (2024 Nov 7). The variable structural flexibility of the Bacillus circulans β-galactosidase isoforms determines their unique functionalities. Structure (London, England : 1993), 32(11), 2023-2037.e5. doi: 10.1016/j.str.2024.09.005. ID: 39353423.


3.) Hoboth P, Sztacho M, Hozák P (2024 Oct). Nuclear patterns of phosphatidylinositol 4,5- and 3,4-bisphosphate revealed by super-resolution microscopy differ between the consecutive stages of RNA polymerase II transcription. The FEBS journal, 291(19), 4240-4264. doi: 10.1111/febs.17136. ID: 38734927.


4.) Vašek D, Fikarová N, Marková VN, Honc O, Pacáková L, Porubská B, Somova V, Novotný J, Melkes B, Krulová M (2024 May 24). Lipopolysaccharide pretreatment increases the sensitivity of the TRPV1 channel and promotes an anti-inflammatory phenotype of capsaicin-activated macrophages. Journal of inflammation (London, England), 21(1), 17. . ID: 38790047.


5.) Huebbers JW, Caldarescu GA, Kubátová Z, Sabol P, Levecque SCJ, Kuhn H, Kulich I, Reinstädler A, Büttgen K, Manga-Robles A, Mélida H, Pauly M, Panstruga R, Žárský V (2024 Mar 29). Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. The Plant cell, 36(4), 1007-1035. . ID: 38124479.


6.) Majer J, Kindermann M, Pinkas D, Chvatil D, Cigler P, Libusova L (2024 Feb 1). Cellular uptake and fate of cationic polymer-coated nanodiamonds delivering siRNA: a mechanistic study. Nanoscale, 16(5), 2490-2503. doi: 10.1039/d3nr05738k. ID: 38197438.


7.) Schrecengost A, Rotterová J, Poláková K, Čepička I, Beinart RA (2024 Jan 8). Divergent marine anaerobic ciliates harbor closely related Methanocorpusculum endosymbionts. The ISME journal, 18(1), . . ID: 38982749.


8.) Shewale MK, Nebesářová J, Grosse-Wilde E, Kalinová B (2023 Dec). Microscopic morphology and distribution of the antennal sensilla in the double-spined bark beetle, Ips duplicatus (Coleoptera: Curculionidae). Microscopy research and technique, 86(12), 1610-1625. doi: 10.1002/jemt.24397. ID: 37572001.


9.) Filova E, Supova M, Eckhardt A, Vrbacky M, Blanquer A, Travnickova M, Knitlova J, Suchy T, Ryglova S, Braun M, Burdikova Z, Schätz M, Jencova V, Lisnenko M, Behalek L, Prochazkova R, Sedlacek R, Kubasova K, Bacakova L (2023 Mar 16). Adipose-Derived Stem Cells in Reinforced Collagen Gel: A Comparison between Two Approaches to Differentiation towards Smooth Muscle Cells. International journal of molecular sciences, 24(6), . . ID: 36982766.


10.) Rattay S, Hufbauer M, Hoboth P, Sztacho M, Akgül B (2023 Mar). Viruses and phospholipids: Friends and foes during infection. Journal of medical virology, 95(3), e28658. doi: 10.1002/jmv.28658. ID: 36905213.


11.) Měřička P, Janoušek L, Benda A, Lainková R, Sabó J, Dalecká M, Prokšová P, Salmay M, Špunda R, Pecha O, Jandová M, Gregor J, Štěrba L, Špaček M, Lindner J (2021 Sep 30). Cell Viability Assessment Using Fluorescence Vital Dyes and Confocal Microscopy in Evaluating Freezing and Thawing Protocols Used in Cryopreservation of Allogeneic Venous Grafts. International journal of molecular sciences, 22(19), . . ID: 34638994.


12.) Dalecká M, Sabó J, Backová L, Rösel D, Brábek J, Benda A, Tolde O (2021 Jul 21). Invadopodia Structure in 3D Environment Resolved by Near-Infrared Branding Protocol Combining Correlative Confocal and FIB-SEM Microscopy. International journal of molecular sciences, 22(15), . . ID: 34360570.


Description:

Welcome to the Open Access Microscopy Core Facility, where cutting-edge imaging technology is made available to researchers in various disciplines. Our state-of-the-art facility is equipped with a range of advanced microscopes, from traditional light microscopes to cutting-edge super-resolution and live cell imaging systems. Our mission is to provide researchers with the tools and expertise they need to make breakthrough discoveries without the limitations imposed by traditional, expensive core facilities. With open access to the latest equipment and a team of highly trained imaging specialists, we are dedicated to advancing the frontiers of scientific discovery. Whether you are a seasoned researcher or just starting, we support you and your research needs.