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Montana State University - Cellular Analysis Core

Montana State University

Contact Info:

Andy Sebrell

Health Sciences Building 247 and 241

Bozeman, MT 59717

https://www.montana.edu/mbi/facilities/cellular-analysis-core/index.html

Grants and Identifiers:

RRID: RRID:SCR_026328

Instrumentation:

BD LSRFortessa Fortessa Flow Cytometer

BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]

RRID:SCR_019601

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Bio-Rad ZE5 Cell Analyzer

Cytometer with configurations to meet broad range of experimental complexities and throughput needs. Accessible for novice flow cytometry users yet flexible enough for most experienced flow cytometry professionals. [Product Link]

RRID:SCR_019712

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10X Genomics Chromium Controller

BD FACS Aria II Cell Sorter

ImageStream Imaging Cytometer

Stratedigm SE520EON

Services Provided:

Flow Cytometry

Histology

Single Cell RNA Sequencing

Relevant Publications:

1.) Luu AM, Hatton AA, Gattiker J, Shepardson KM, Hall MN, Benson E, Bimczok D, Rynda-Apple A (2025 May 28). TLR2 or TLR4 stimulation induces transmembrane (tm)TNF-driven priming in macrophages which results in improved clearance of a subsequent Staphylococcus aureus infection. Journal of innate immunity, (), 1-28. doi: 10.1159/000546011. ID: 40435995.


2.) Predtechenskaya M, Arbizzani CJ, Shomento SR, Borgogna TR, Voyich JM (2025 Apr 16). Lung surfactant reduces Staphylococcus aureus cytotoxicity and protects host immune cells from membrane damage. Microbiology spectrum, (), e0138624. doi: 10.1128/spectrum.01386-24. ID: 40237467.


3.) Cherne MD, Snyder D, Sidar B, Blackwell K, Jenkins B, Huang S, Sebrell TA, Hedges JF, Spence JR, Chang CB, Wilking JN, Walk ST, Jutila MA, Loveday EK, Bimczok D (2025 Mar 28). Strain- and vaccine-specific effects of serum antibodies in the protection of intestinal SARS-CoV-2 infection. medRxiv : the preprint server for health sciences, (), . . ID: 40196264.


4.) Jacobson BT, DeWit-Dibbert J, Zanca L, Sonar S, Hardy C, Throolin M, Brewster PC, Andujo K, Jones K, Sago J, Smith S, Bowen L, Bimczok D (2025 Jan 13). Pathogen delivery route impacts disease severity in experimental Mycoplasma ovipneumoniae infection of domestic lambs. Veterinary research, 56(1), 10. . ID: 39806398.


5.) Trauner AM, McCoski SR, Satterfield MC, Bradbery AN (2025 Jan 4). Late gestation maternal overnutrition alters gene expression and histomorphology in neonatal foal testes. Journal of animal science, 103(), . . ID: 40036360.


6.) Jacobson BT, DeWit-Dibbert J, Selong ET, Quirk M, Throolin M, Corona C, Sonar S, Zanca L, Schwarz ER, Bimczok D (2024 Dec 20). Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms. Microorganisms, 12(12), . . ID: 39770853.


7.) Gaur G, Predtechenskaya M, Voyich JM, James G, Stewart PS, Borgogna TR (2024 Sep 27). Assessing the Effects of Surgical Irrigation Solutions on Human Neutrophil Interactions with Nascent Staphylococcus aureus Biofilms. Microorganisms, 12(10), . . ID: 39458262.


8.) Nygaard TK, Borgogna TR, Pallister KB, Predtechenskaya M, Burroughs OS, Gao A, Lubick EG, Voyich JM (2024 Aug 28). The Relative Importance of Cytotoxins Produced by Methicillin-Resistant Staphylococcus aureus Strain USA300 for Causing Human PMN Destruction. Microorganisms, 12(9), . . ID: 39338457.


Description:

The Cellular Analysis Core (CAC) provides access to functional, up-to-date equipment for flow cytometric analysis and cell sorting, histological samples preparation, and single cell RNA sequencing which is essential for all researchers in immunology, infectious disease, and cell biology and is highly useful for scientists in other related fields.