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YU-Keck MS & Proteomics Resource

Yale University

Contact Info:

300 George Street Suite G001

New Haven, CT 06510

https://research.yale.edu/cores/keck-mass-spectrometry-proteomics-resource

Grants and Identifiers:

RRID: RRID:SCR_026192

Other Citation Identifiers

We also thank the Keck MS & Proteomics Resource at the Yale School of Medicine for providing the nec

Instrumentation:

Thermo Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer

Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer has increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies. [Product Link]

RRID:SCR_020556

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Thermo Scientific Orbitrap Fusion Tribrid Mass Spectrometer

Combines quadrupole, ion trap, and Orbitrap mass analysis into Tribrid architecture to provide depth of analysis. Enables analyzing of low-abundance, high-complexity samples to identify compounds and to quantify accurately and elucidate structures thoroughly. [Product Link]

RRID:SCR_020573

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Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer

HFX is our newest addition to the proteomics resource. It offers the fastest scanning rate of our Orbitraps, which improves proteomic depth for discovery applications, and integrates well with targeted applications such as parallel reaction monitoring. [Product Link]

RRID:SCR_020555

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Thermo Scientific Orbitrap Exploris 480

Quadrupole-Orbitrap Mass Spectreometer

Services Provided:

High-resolution (HR) MS For Small Molecule And Structural Elucidation

Protein ID From Complex Solution (e.g. Tissue, Cells, Biological Fluids, Etc.)

Protein ID From Gel Bands Or Immunoprecipitated Workflow

Protein Post-translational Modification (PTM) Identification

Protein Profiling And Quantitation (Label-Free Quantitation, TMT, SILAC)

Targeted Proteomics (PRM)

Targeted Small Molecule Quantitation (MRM)

Relevant Publications:

1.) Johnson B, Iuliano M, Lam TT, Biederer T, De Camilli PV (2024 Nov 4). A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell-cell contacts. The Journal of cell biology, 223(11), . . ID: 39158698.


2.) Singh P, Choi JY, Wang W, T Lam T, Lechner P, Vanderwal CD, Pou S, Nilsen A, Ben Mamoun C (2024 Nov). A fluorescence-based assay for measuring polyamine biosynthesis aminopropyl transferase-mediated catalysis. The Journal of biological chemistry, 300(11), 107832. . ID: 39342998.


3.) Zhu B, Park JM, Coffey SR, Russo A, Hsu IU, Wang J, Su C, Chang R, Lam TT, Gopal PP, Ginsberg SD, Zhao H, Hafler DA, Chandra SS, Zhang L (2024 Oct 30). Single-cell transcriptomic and proteomic analysis of Parkinson Science translational medicine, 16(771), eabo1997. doi: 10.1126/scitranslmed.abo1997. ID: 39475571.


4.) Lallai V, Lam TT, Garcia-Milian R, Chen YC, Fowler JP, Manca L, Piomelli D, Williams K, Nairn AC, Fowler CD (2024 Sep 10). Proteomic Profile of Circulating Extracellular Vesicles in the Brain after Δ9-Tetrahydrocannabinol Inhalation. Biomolecules, 14(9), . . ID: 39334909.


5.) Gordon JM, Phizicky DV, Schärfen L, Brown CL, Arias Escayola D, Kanyo J, Lam TT, Simon MD, Neugebauer KM (2024 Sep 9). Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics. Nucleic acids research, 52(16), 9886-9903. . ID: 38943343.


6.) Chanduri M, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz UD, Bai S, Nottoli T, Goult BT, Humphrey JD, Schwartz MA (2024 Aug 23). Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis. Science advances, 10(34), eadi6286. . ID: 39167642.


7.) Norton ES, Whaley LA, Jones VK, Brooks MM, Russo MN, Morderer D, Jessen E, Schiapparelli P, Ramos-Fresnedo A, Zarco N, Carrano A, Rossoll W, Asmann YW, Lam TT, Chaichana KL, Anastasiadis PZ, Quiñones-Hinojosa A, Guerrero-Cázares H (2024 Aug 9). Cell-specific cross-talk proteomics reveals cathepsin B signaling as a driver of glioblastoma malignancy near the subventricular zone. Science advances, 10(32), eadn1607. . ID: 39110807.


8.) Pelletier SJ, Leclercq M, Roux-Dalvai F, de Geus MB, Leslie S, Wang W, Lam TT, Nairn AC, Arnold SE, Carlyle BC, Precioso F, Droit A (2024 May 6). BERNN: Enhancing classification of Liquid Chromatography Mass Spectrometry data with batch effect removal neural networks. Nature communications, 15(1), 3777. . ID: 38710683.


9.) Mansuri MS, Bathla S, Lam TT, Nairn AC, Williams KR (2024 Apr 15). Optimal conditions for carrying out trypsin digestions on complex proteomes: From bulk samples to single cells. Journal of proteomics, 297(), 105109. . ID: 38325732.


10.) Ryu HY, Jeong DW, Kim SY, Jeoung SW, Zhao D, Knight J, Lam T, Jin JH, Lee HS, Hochstrasser M (2024 Mar 21). Auto-sumoylation of the Ubc9 E2 SUMO-conjugating Enzyme Extends Cellular Lifespan. Research square, (), . . ID: 38562857.


11.) Shi X, DeCiucis M, Grabinska KA, Kanyo J, Liu A, Lam TT, Shen H (2024 Feb 15). Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis. Molecular cell, 84(4), 802-810.e6. . ID: 38157846.


12.) Hofford RS, Meckel KR, Wiser EJ, Wang W, Sens JP, Kim M, Godino A, Lam TT, Kiraly DD (2024 Feb). Microbiome Depletion Increases Fentanyl Self-Administration and Alters the Striatal Proteome Through Short-Chain Fatty Acids. eNeuro, 11(2), . . ID: 38164564.


13.) Yang ML, Lam TT, Kanyo J, Kang I, Zhou ZS, Clarke SG, Mamula MJ (2023 Dec). Natural isoaspartyl protein modification of ZAP70 alters T cell responses in lupus. Autoimmunity, 56(1), 2282945. . ID: 37994408.


14.) Misra S, Khan EI, Lam TT, Mazumder R, Gururangan K, Hickman LB, Goswami V, Funaro MC, Eldem E, Sansing LH, Sico JJ, Quinn TJ, Liebeskind DS, Montaner J, Kwan P, Mishra NK (2023 Nov 27). Common Pathways of Epileptogenesis in Patients With Epilepsy Post-Brain Injury: Findings From a Systematic Review and Meta-analysis. Neurology, 101(22), e2243-e2256. . ID: 37550071.


15.) Wang Y, Popovic Z, Charkoftaki G, Garcia-Milian R, Lam TT, Thompson DC, Chen Y, Vasiliou V (2023 Oct 1). Multi-omics profiling reveals cellular pathways and functions regulated by ALDH1B1 in colon cancer cells. Chemico-biological interactions, 384(), 110714. . ID: 37716420.


Description:

The Keck Mass Spectrometry (MS) & Proteomics Resource at the Yale School of Medicine provides efficient and cost-effective proteomics and small molecule mass spectrometry-based services. Beside from our developed Standard Operating Procedure (SOP) offered for many of our MS services, we also strive to continue to bring state-of-the-art mass spectrometry-based biotechnologies through input and request from our users. These technologies are developed and implemented with a broad spectrum of mass spectrometry-based techniques and protein/peptide/small molecule chemistries to separate, characterize, profile, and quantify analytes from complex biological samples.