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Advanced Microscopy Applications Unit (ADMiRA), RAI, UNAM

Instituto Nacional de Cancerología

Contact Info:

Alejandro Lopez-Saavedra

Av. San Fernando 22. Col, Belisario Domínguez Sección XVI

Tlalpan, Mexico City

https://admiramicro.com.mx

Grants and Identifiers:

RRID: RRID:SCR_026170

Instrumentation:

ZEISS Axio Scan.Z1 Slide Scanner

Digitize your specimens with the Axio Scan.Z1 slide scanner and create high-quality virtual slides. Axio Scan.Z1 tackles the most demanding virtual microscopy research tasks as easily as it handles your routine work. The software module ZEN slidescan is designed specifically for the workflow of capturing virtual slides, while ZEN image analysis tools prepare your data accurately. Organize your virtual slides with ZEN browser, the web-based database, then view your data from any location using any operating system , or share virtual microscopy images online with colleagues and organize your projects, even when you are on the go. [Product Link]

RRID:SCR_020927

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Zeiss Axio Vert series Axiovert 200 inverted microscope

Inverted microscope that is used for the examination of cell and tissue culture of sediments in culture flasks, Petri dishes, microtiter plates, etc. in transmitted and reflected light. It hass attachment possibilities for various tools (micromanipulation), different light sources, temperature control devices. [Product Link]

RRID:SCR_020915

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Zeiss Lightsheet Z.1 Lightsheet Fluorescence Microscope

Light sheet fluorescence microscope for the imaging of large cleared specimens. Lightsheet Z.1 with Clr Plan-Apochromat 20x/1.0 Corr nd=1.38 is used to perform experiments with tissue cleared by Scale medium (Hama et al, Nat Neurosci. 2011), which has a refractive index of n=1.38. [Product Link]

RRID:SCR_020919

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Zeiss LSM 710 Confocal Inverted Microscope

LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]

RRID:SCR_018063

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Zeiss PALM MicroBeam PALM

PALM MicroBeam scanning electron microscope for isolating uncontaminated source material simple. [Product Link]

RRID:SCR_020929

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Zeiss PALM MicroTweezers

Optical tweezers system for contact free cell manipulation as well as to trap, move, and sort microscopic particles, sort live cells, organelles, and other large biomolecules. [Product Link]

RRID:SCR_018647

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ZEISS AURIGA

STEM, FIB

ZEISS Discovery V.12

Stereomicroscope

ZEISS Elyra PS.1

Super resolution:SIM, PALM, TIRF

ZEISS METAFER

Slide scanner for cytogenetic analysis

Services Provided:

Advanced Microscopy Applications: Confocal, Super Resolution, Slide Scanning, Microdisection, Lightsheet, STEM And FIB Microscopy

Correlative Light Electron Microscopy

Fluorescence Recovery After Photobleaching (FRAP)

Forster Resonance Energy Transfer (FRET)

Live Cell Imaging

Multiphoton Microscopy

Optical Tweezers

PALM Microscopy

Structured Illumination Microscopy

TIRF Microscopy

Relevant Publications:

1.) Jabloñski M, Luque GM, Gomez Elias M, Sanchez Cardenas C, Xu X, de La Vega Beltran JL, Corkidi G, Linares A, Abonza V, Arenas-Hernandez A, Ramos-Godinez MDP, López-Saavedra A, Krapf D, Krapf D, Darszon A, Guerrero A, Buffone MG (2024 Nov 13). Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization. eLife, 13(), . . ID: 39535529.


2.) Delgado-Waldo I, Dokudovskaya S, Loissell-Baltazar YA, Pérez-Arteaga E, Coronel-Hernández J, Martínez-Vázquez M, Pérez-Yépez EA, Lopez-Saavedra A, Jacobo-Herrera N, Pérez Plasencia C (2024 Oct 3). Laherradurin Inhibits Colorectal Cancer Cell Growth by Induction of Mitochondrial Dysfunction and Autophagy Induction. Cells, 13(19), . . ID: 39404412.


3.) Jabloñski M, Luque GM, Gómez-Elías MD, Sanchez-Cardenas C, Xu X, de la Vega-Beltran JL, Corkidi G, Linares A, Abonza Amaro VX, Arenas-Hernandez A, Del Pilar Ramos-Godinez M, López-Saavedra A, Krapf D, Krapf D, Darszon A, Guerrero A, Buffone MG (2024 Jul 2). Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization. bioRxiv : the preprint server for biology, (), . . ID: 37904966.


4.) Carbajal-Contreras H, Murillo-de-Ozores AR, Magaña-Avila G, Marquez-Salinas A, Bourqui L, Tellez-Sutterlin M, Bahena-Lopez JP, Cortes-Arroyo E, Behn-Eschenburg SG, Lopez-Saavedra A, Vazquez N, Ellison DH, Loffing J, Gamba G, Castañeda-Bueno M (2024 Feb 1). Arginine vasopressin regulates the renal Na(+)-Cl(-) and Na(+)-K(+)-Cl(-) cotransporters through with-no-lysine kinase 4 and inhibitor 1 phosphorylation. American journal of physiology. Renal physiology, 326(2), F285-F299. . ID: 38096266.


5.) Justo-Garrido M, López-Saavedra A, Alcaraz N, Cortés-González CC, Oñate-Ocaña LF, Caro-Sánchez CHS, Castro-Hernández C, Arriaga-Canon C, Díaz-Chávez J, Herrera LA (2023 Nov 9). Association of SLC12A1 and GLUR4 Ion Transporters with Neoadjuvant Chemoresistance in Luminal Locally Advanced Breast Cancer. International journal of molecular sciences, 24(22), . . ID: 38003293.


6.) Ortiz-Pedraza Y, Muñoz-Bello JO, Ramos-Chávez LA, Martínez-Ramírez I, Olmedo-Nieva L, Manzo-Merino J, López-Saavedra A, Pérez-de la Cruz V, Lizano M (2023 Jan 24). HPV16 E6 and E7 Oncoproteins Stimulate the Glutamine Pathway Maintaining Cell Proliferation in a SNAT1-Dependent Fashion. Viruses, 15(2), . . ID: 36851539.


7.) Murillo-de-Ozores AR, Carbajal-Contreras H, Magaña-Ávila GR, Valdés R, Grajeda-Medina LI, Vázquez N, Zariñán T, López-Saavedra A, Sharma A, Lin DH, Wang WH, Delpire E, Ellison DH, Gamba G, Castañeda-Bueno M (2022 Nov). Multiple molecular mechanisms are involved in the activation of the kidney sodium-chloride cotransporter by hypokalemia. Kidney international, 102(5), 1030-1041. . ID: 35870644.


8.) Andonegui-Elguera MA, Cáceres-Gutiérrez RE, López-Saavedra A, Cisneros-Soberanis F, Justo-Garrido M, Díaz-Chávez J, Herrera LA (2022 Aug 5). The Roles of Histone Post-Translational Modifications in the Formation and Function of a Mitotic Chromosome. International journal of molecular sciences, 23(15), . . ID: 35955838.


9.) Colin-Val Z, Vera-Márquez CD, Herrera-Rodríguez MA, Del Pilar Ramos-Godinez M, López-Saavedra A, Cano-Martínez A, Robledo-Cadena DX, Rodríguez-Enríquez S, Correa F, Delgado-Buenrostro NL, Chirino YI, López-Marure R (2022 Aug). Titanium Dioxide (E171) Induces Toxicity in H9c2 Rat Cardiomyoblasts and Ex Vivo Rat Hearts. Cardiovascular toxicology, 22(8), 713-726. doi: 10.1007/s12012-022-09747-5. ID: 35633469.


10.) Martínez-González JJ, Ríos-Morales SL, Guevara-Flores A, Ramos-Godinez MDP, López-Saavedra A, Rendón JL, Del Arenal Mena IP (2022 Aug). Evaluating the effect of curcumin on the metacestode of Taenia crassiceps. Experimental parasitology, 239(), 108319. doi: 10.1016/j.exppara.2022.108319. ID: 35777452.


11.) Fabian-Morales E, Vallejo-Escamilla D, Gudiño A, Rodríguez A, González-Barrios R, Rodríguez Torres YL, Castro Hernández C, de la Torre-Luján AH, Oliva-Rico DA, Ornelas Guzmán EC, López Saavedra A, Frias S, Herrera LA (2022 May 1). Large-scale topological disruption of chromosome territories 9 and 22 is associated with nonresponse to treatment in CML. International journal of cancer, 150(9), 1455-1470. . ID: 34913480.


12.) Cáceres-Gutiérrez RE, Andonegui MA, Oliva-Rico DA, González-Barrios R, Luna F, Arriaga-Canon C, López-Saavedra A, Prada D, Castro C, Parmentier L, Díaz-Chávez J, Alfaro-Mora Y, Navarro-Delgado EI, Fabian-Morales E, Tran B, Shetty J, Zhao Y, Alcaraz N, De la Rosa C, Reyes JL, Hédouin S, Hubé F, Francastel C, Herrera LA (2022 Apr). Proteasome inhibition alters mitotic progression through the upregulation of centromeric α-Satellite RNAs. The FEBS journal, 289(7), 1858-1875. . ID: 34739170.


13.) Saldivar-Cerón HI, Villamar-Cruz O, Wells CM, Oguz I, Spaggiari F, Chernoff J, Patiño-López G, Huerta-Yepez S, Montecillo-Aguado M, Rivera-Pazos CM, Loza-Mejía MA, Vivar-Sierra A, Briseño-Díaz P, Zentella-Dehesa A, Leon-Del-Rio A, López-Saavedra A, Padierna-Mota L, Ibarra-Sánchez MJ, Esparza-López J, Hernández-Rivas R, Arias-Romero LE (2021). p21-Activated Kinase 1 Promotes Breast Tumorigenesis via Phosphorylation and Activation of the Calcium/Calmodulin-Dependent Protein Kinase II. Frontiers in cell and developmental biology, 9(), 759259. . ID: 35111748.


Description:

Facility devoted to answer scientific, industrial and medical questions by providing technical and scientific support to all kind of users in advanced microscopy and image processing and analysis