Montana State University

Contact Info:

Martin Lawrence

Room 24, Chemistry & Biochemistry Building

Bozeman, MT 59717

https://www.montana.edu/cryo-electron-microscopy/

Grants and Identifiers:

RRID: RRID:SCR_026324

Instrumentation:

Services Provided:

Cryo-Electron Microscopy

Cryo-Electron Tomography

Single Particle Analysis

Structural Biology

Relevant Publications:

1.) George JT, Burman N, Wilkinson RA, de Silva S, McKelvey-Pham Q, Buyukyoruk M, Dale A, Landman H, Graham A, DeLuca SZ, Wiedenheft B (2025 Mar 26). Structural basis of antiphage defense by an ATPase-associated reverse transcriptase. bioRxiv : the preprint server for biology, (), . . ID: 40196496.

2.) Tang S, Žedaveinytė R, Burman N, Pandey S, Ramirez JL, Kulber LM, Wiegand T, Wilkinson RA, Ma Y, Zhang DJ, Lampe GD, Berisa M, Jovanovic M, Wiedenheft B, Sternberg SH (2025 Mar 25). Protein-primed DNA homopolymer synthesis by an antiviral reverse transcriptase. bioRxiv : the preprint server for biology, (), . . ID: 40196691.

3.) Sobolewski TN, Trousdale RC, Gauvin CL, Lawrence CM, Walker RA (2025 Jan 14). Nanomolar PFOA Concentrations Affect Lipid Membrane Structure: Consequences for Bioconcentration Mechanisms. Environmental science & technology, 59(1), 709-718. doi: 10.1021/acs.est.4c03652. ID: 39718541.

4.) Burman N, Belukhina S, Depardieu F, Wilkinson RA, Skutel M, Santiago-Frangos A, Graham AB, Livenskyi A, Chechenina A, Morozova N, Zahl T, Henriques WS, Buyukyoruk M, Rouillon C, Saudemont B, Shyrokova L, Kurata T, Hauryliuk V, Severinov K, Groseille J, Thierry A, Koszul R, Tesson F, Bernheim A, Bikard D, Wiedenheft B, Isaev A (2024 Dec). Author Correction: A virally encoded tRNA neutralizes the PARIS antiviral defence system. Nature, 636(8043), E8. . ID: 39633059.

5.) Sobolewski TN, Findlay JL, Hemphill JE, Walker RA (2024 Nov 26). Aggregation, Not Micellization: Perfluorooctanoic Acid, Perfluorobutanesulfonic Acid, and Potassium Perfluorooctanesulfonate Behavior in Aqueous Solution. Langmuir : the ACS journal of surfaces and colloids, 40(47), 24820-24831. doi: 10.1021/acs.langmuir.4c02566. ID: 39539066.

6.) Burman N, Belukhina S, Depardieu F, Wilkinson RA, Skutel M, Santiago-Frangos A, Graham AB, Livenskyi A, Chechenina A, Morozova N, Zahl T, Henriques WS, Buyukyoruk M, Rouillon C, Saudemont B, Shyrokova L, Kurata T, Hauryliuk V, Severinov K, Groseille J, Thierry A, Koszul R, Tesson F, Bernheim A, Bikard D, Wiedenheft B, Isaev A (2024 Oct). A virally encoded tRNA neutralizes the PARIS antiviral defence system. Nature, 634(8033), 424-431. . ID: 39111359.

7.) Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B (2023 Nov). Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays. Nature structural & molecular biology, 30(11), 1675-1685. . ID: 37710013.

Description:

The Cryo-EM core facility at MSU is a regional facility that brings state of the art cryo-EM capabilities, including single particle analysis and cryo-electron tomography, to the Northern Rocky Mountains. Instrumentation includes a 200 kV Talos Arctica with a Gatan K3 camera, 120 kV Tecnai Spirit, and a Vitrobot Mark IV. Collectively, these instruments allow imaging of biological structures at single nanometer to sub-nanometer resolutions, and thus enable 3D modeling of cells, viruses, proteins, nucleic acid, and other soft matter at the atomic level.