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Biological Mass Spectrometry and Proteomics

Dartmouth Cancer Center

Contact Info:

Anna Schmoker

1 Medical Center Drive

Rubin 7

Lebanon, NH 02769

https://geiselmed.dartmouth.edu/msp/

Grants and Identifiers:

RRID: RRID:SCR_026076

Instrumentation:

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer

Spectrometer performs analysis including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, and MSn analysis of small molecules. System incorporates the brightest ion source, segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology, and ETD HD. [Product Link]

RRID:SCR_020562

cite this instrument

Thermo Scientific Orbitrap Fusion Tribrid Mass Spectrometer

Combines quadrupole, ion trap, and Orbitrap mass analysis into Tribrid architecture to provide depth of analysis. Enables analyzing of low-abundance, high-complexity samples to identify compounds and to quantify accurately and elucidate structures thoroughly. [Product Link]

RRID:SCR_020573

cite this instrument

Thermo Scientific Q Exactive Plus Mass Spectrometer

(Equipment No Longer Active At this Facility)

Thermo Scientific Thermo Scientific Q Exactive Plus Mass Spectrometer

Services Provided:

Affinity Purification Mass Spectrometry (APMS)

Crosslinking Mass Spectrometry

Gel Band Identification

Intact Protein Mass Spectrometry

Interactomics

Label Free Quantitative Proteomics

Mass Spectrometry - Proteins And Peptides

Post Translational Modification Analysis

Proteomics

Quantitative Phosphoproteomics

Quantitative Proteomics - TMT

Targeted Peptide Quantification

Relevant Publications:

1.) Muranaka F, Kise E, Tokumaru S, Kitazawa M, Miyagawa Y, Suga T, Uehara T, Iwaya M, Kobayashi S, Sato M, Gomi D, Yamada H, Sugimura H, Kosho T, Soejima Y, Koizumi T (2023 Jan 31). Hereditary diffuse gastricĀ cancerĀ in a Japanese family with CDH1 mutation three case reports. Discover oncology, 14(1), 14. . PMID: 36719602.


2.) Sun H, Du Y, Kumar R, Buchkovich N, He P (2022 Dec 1). Increased circulating microparticles contribute to severe infection and adverse outcomes of COVID-19 in patients with diabetes. American journal of physiology. Heart and circulatory physiology, 323(6), H1176-H1193. . PMID: 36269646.


3.) Bao L, Festa F, Freet CS, Lee JP, Hirschler-Laszkiewicz IM, Chen SJ, Keefer KA, Wang HG, Patterson AD, Cheung JY, Miller BA (2019 Oct 1). The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2. Scientific reports, 9(1), 14132. . PMID: 31575956.


4.) Bowser BS, Chen HS, Conway MJ, Christensen ND, Meyers C (2011 Sep). Human papillomavirus type 18 chimeras containing the L2/L1 capsid genes from evolutionarily diverse papillomavirus types generate infectious virus. Virus research, 160(1), 246-55. . PMID: 21762735.


5.) Jones NC, Martin S, Megatia I, Hakami T, Salzberg MR, Pinault D, Morris MJ, O'Brien TJ, van den Buuse M (2010 Jul). A genetic epilepsy rat model displays endophenotypes of psychosis. Neurobiology of disease, 39(1), 116-25. doi: 10.1016/j.nbd.2010.02.001. PMID: 20153428.


6.) Jones NC, Kumar G, O'Brien TJ, Morris MJ, Rees SM, Salzberg MR (2009 Oct 12). Anxiolytic effects of rapid amygdala kindling, and the influence of early life experience in rats. Behavioural brain research, 203(1), 81-7. doi: 10.1016/j.bbr.2009.04.023. PMID: 19397932.


Description:

We offer quantitative and qualitative proteomics services using LC-MS/MS technologies. Our rates include sample preparation from harvested cell pellets, elutions post-enrichment or purification, or gel bands through data acquisition, quantification, and statistical analysis.