Harvard Medical School
250 Longwood Ave, Boston MA 02115
Seeley G. Mudd B04
Boston, MA 02115
https://cryoem.hms.harvard.edu
RRID: RRID:SCR_025841
(Equipment No Longer Active At this Facility)
FEI Talos Arctica Cryo-TEM
Transmission electron microscope that is built for delivering high-resolution 3D characterization of biological samples and biomaterials in cell biology, structural biology, and nanotechnology research. [Product Link]
RRID:SCR_019905
(Equipment No Longer Active At this Facility)
FEI FEI Titan Krios G3i
Third Generation Titan Krios
FEI Talos Arctica
Talos Arctica with K3 Detector
(Equipment No Longer Active At this Facility)
FEI Titan Krios G3i
Titan Krios1- with Falcon4i direct detector and Selectors Energy Filter
FEI Titan Krios G3i
Titan Krios2 with Falcon4i Direct Detector and Selectors Energy Filter
1.) Xiao L, Pi X, Goss AC, El-Baba T, Ehrmann JF, Grinkevich E, Bazua-Valenti S, Padovano V, Alper SL, Carey D, Udeshi ND, Carr SA, Pablo JL, Robinson CV, Greka A, Wu H (2024 Sep 20). Molecular basis of TMED9 oligomerization and entrapment of misfolded protein cargo in the early secretory pathway. Science advances, 10(38), eadp2221. . ID: 39303030.
2.) Shankar S, Pan J, Yang P, Bian Y, Oroszlán G, Yu Z, Mukherjee P, Filman DJ, Hogle JM, Shekhar M, Coen DM, Abraham J (2024 Aug 26). Viral DNA polymerase structures reveal mechanisms of antiviral drug resistance. Cell, (), . pii: S0092-8674(24)00842-0. doi: 10.1016/j.cell.2024.07.048. ID: 39197451.
3.) Yang P, Li W, Fan X, Pan J, Mann CJ, Varnum H, Clark LE, Clark SA, Coscia A, Basu H, Smith KN, Brusic V, Abraham J (2024 Aug 2). Structural basis for VLDLR recognition by eastern equine encephalitis virus. Nature communications, 15(1), 6548. . ID: 39095394.
4.) Velez B, Walsh RM Jr, Rawson S, Razi A, Adams L, Perez EF, Jiao F, Blickling M, Rajakumar T, Fung D, Huang L, Hanna J (2024 Aug). Mechanism of autocatalytic activation during proteasome assembly. Nature structural & molecular biology, 31(8), 1167-1175. doi: 10.1038/s41594-024-01262-1. ID: 38600323.
5.) Frank HM, Walujkar S, Walsh RM Jr, Laursen WJ, Theobald DL, Garrity PA, Gaudet R (2024 Apr 23). Structural basis of ligand specificity and channel activation in an insect gustatory receptor. Cell reports, 43(4), 114035. . ID: 38573859.
6.) Johnson AG, Mayer ML, Schaefer SL, McNamara-Bordewick NK, Hummer G, Kranzusch PJ (2024 Apr). Structure and assembly of a bacterial gasdermin pore. Nature, 628(8008), 657-663. doi: 10.1038/s41586-024-07216-3. ID: 38509367.
7.) Finney J, Moseman AP, Kong S, Watanabe A, Song S, Walsh RM Jr, Kuraoka M, Kotaki R, Moseman EA, McCarthy KR, Liao D, Liang X, Nie X, Lavidor O, Abbott R, Harrison SC, Kelsoe G (2024 Jan 2). Protective human antibodies against a conserved epitope in pre- and postfusion influenza hemagglutinin. Proceedings of the National Academy of Sciences of the United States of America, 121(1), e2316964120. . ID: 38147556.
8.) Walsh RM Jr, Rawson S, Schnell HM, Velez B, Rajakumar T, Hanna J (2023 Oct). Structure of the preholoproteasome reveals late steps in proteasome core particle biogenesis. Nature structural & molecular biology, 30(10), 1516-1524. . ID: 37653242.
9.) Shi W, Cai Y, Zhu H, Peng H, Voyer J, Rits-Volloch S, Cao H, Mayer ML, Song K, Xu C, Lu J, Zhang J, Chen B (2023 Jul). Cryo-EM structure of SARS-CoV-2 postfusion spike in membrane. Nature, 619(7969), 403-409. doi: 10.1038/s41586-023-06273-4. ID: 37285872.
10.) Zhang J, Tang W, Gao H, Lavine CL, Shi W, Peng H, Zhu H, Anand K, Kosikova M, Kwon HJ, Tong P, Gautam A, Rits-Volloch S, Wang S, Mayer ML, Wesemann DR, Seaman MS, Lu J, Xiao T, Xie H, Chen B (2023 Jul). Structural and functional characteristics of the SARS-CoV-2 Omicron subvariant BA.2 spike protein. Nature structural & molecular biology, 30(7), 980-990. doi: 10.1038/s41594-023-01023-6. ID: 37430064.
11.) Duncan-Lowey B, Tal N, Johnson AG, Rawson S, Mayer ML, Doron S, Millman A, Melamed S, Fedorenko T, Kacen A, Brandis A, Mehlman T, Amitai G, Sorek R, Kranzusch PJ (2023 Mar 2). Cryo-EM structure of the RADAR supramolecular anti-phage defense complex. Cell, 186(5), 987-998.e15. . ID: 36764290.
12.) Velilla JA, Volpe MR, Kenney GE, Walsh RM Jr, Balskus EP, Gaudet R (2023 Feb). Structural basis of colibactin activation by the ClbP peptidase. Nature chemical biology, 19(2), 151-158. . ID: 36253550.
13.) Rawson S, Walsh RM Jr, Velez B, Schnell HM, Jiao F, Blickling M, Ang J, Bhanu MK, Huang L, Hanna J (2022 Aug). Yeast PI31 inhibits the proteasome by a direct multisite mechanism. Nature structural & molecular biology, 29(8), 791-800. . ID: 35927584.
14.) Xiong X, Tian S, Yang P, Lebreton F, Bao H, Sheng K, Yin L, Chen P, Zhang J, Qi W, Ruan J, Wu H, Chen H, Breault DT, Wu H, Earl AM, Gilmore MS, Abraham J, Dong M (2022 Mar 31). Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors. Cell, 185(7), 1157-1171.e22. . ID: 35259335.
15.) Walsh RM Jr, Mayer ML, Sun CH, Rawson S, Nair R, Sterling SM, Li Z (2022). Practices for running a research-oriented shared cryo-EM facility. Frontiers in molecular biosciences, 9(), 960940. . ID: 36188224.
16.) Zhang J, Xiao T, Cai Y, Lavine CL, Peng H, Zhu H, Anand K, Tong P, Gautam A, Mayer ML, Walsh RM Jr, Rits-Volloch S, Wesemann DR, Yang W, Seaman MS, Lu J, Chen B (2021 Dec 10). Membrane fusion and immune evasion by the spike protein of SARS-CoV-2 Delta variant. Science (New York, N.Y.), 374(6573), 1353-1360. . ID: 34698504.
17.) Cai Y, Zhang J, Xiao T, Lavine CL, Rawson S, Peng H, Zhu H, Anand K, Tong P, Gautam A, Lu S, Sterling SM, Walsh RM Jr, Rits-Volloch S, Lu J, Wesemann DR, Yang W, Seaman MS, Chen B (2021 Aug 6). Structural basis for enhanced infectivity and immune evasion of SARS-CoV-2 variants. Science (New York, N.Y.), 373(6555), 642-648. . ID: 34168070.
18.) Schnell HM, Walsh RM Jr, Rawson S, Kaur M, Bhanu MK, Tian G, Prado MA, Guerra-Moreno A, Paulo JA, Gygi SP, Roelofs J, Finley D, Hanna J (2021 May). Structures of chaperone-associated assembly intermediates reveal coordinated mechanisms of proteasome biogenesis. Nature structural & molecular biology, 28(5), 418-425. . ID: 33846632.
19.) Zhang J, Cai Y, Xiao T, Lu J, Peng H, Sterling SM, Walsh RM Jr, Rits-Volloch S, Zhu H, Woosley AN, Yang W, Sliz P, Chen B (2021 Apr 30). Structural impact on SARS-CoV-2 spike protein by D614G substitution. Science (New York, N.Y.), 372(6541), 525-530. . ID: 33727252.
20.) Cai Y, Zhang J, Xiao T, Peng H, Sterling SM, Walsh RM Jr, Rawson S, Rits-Volloch S, Chen B (2020 Sep 25). Distinct conformational states of SARS-CoV-2 spike protein. Science (New York, N.Y.), 369(6511), 1586-1592. . ID: 32694201.
21.) Kim JJ, Gharpure A, Teng J, Zhuang Y, Howard RJ, Zhu S, Noviello CM, Walsh RM Jr, Lindahl E, Hibbs RE (2020 Sep). Shared structural mechanisms of general anaesthetics and benzodiazepines. Nature, 585(7824), 303-308. . ID: 32879488.
The Harvard Cryo-Electron Microscopy Center for Structural Biology is a joint effort by Harvard Medical School, Dana-Farber Cancer Institute, Boston Children’s Hospital, and Massachusetts General Hospital to provide state-of-the-art cryo-EM instrumentation and expertise for the Harvard structural biology community. This user facility offers consultation and training by staff in specimen preparation, microscope operation, image acquisition, and data analysis.
