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Electron Microscopy Core Facility (EMCF)

The University of Texas Southwestern Medical Center

Contact Info:

Natalia V Gounko

5323 Harry Hines Blvd.

Dallas, TX 75390-9039

https://www.utsouthwestern.edu/research/core-facilities/emcf/

Grants and Identifiers:

RRID: RRID:SCR_012408

Other Citation Identifiers

1S10OD021685-01A1

1S10OD020103-01

Instrumentation:

Jeol JEOL JEM-1400Flash Transmission Electron Microscope

Transmission electron microscope that can be used to first view samples at low magnification before studying fine structures of interest at high magnification. [Product Link]

RRID:SCR_020179

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Leica EM ACE600 High Vacuum Sputter Coater

EM ACE600 sputter coater is a versatile high vacuum film deposition instrument for your FE-SEM and TEM applications. [Product Link]

RRID:SCR_020237

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Leica EM AFS2 Freeze Substitution Device

Leica EM AFS2 is a freeze substitution platform that performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use. [Product Link]

RRID:SCR_020230

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Leica EM FC6 UC6b Ultramicrotome

Leica Ultracut EM UC6 is an advanced ultramicrotome that features a Touch Screen Control Panel or a Keypad Control Panel. Both options enable fast and safer alignment of knife and specimen, and the programmable knife and cutting movements make trimming easy. With Leica's AutoTrim, you can automatically trim a specimen to a predetermined level in the block face, best for morphometric studies. Three dependent, built-in, brightness- controlled LED light sources provide outstanding illumination for top light, back light, and specimen transillumination. [Product Link]

RRID:SCR_020226

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Leica VT1200S vibrating microtome

Fully automated Leica VT1200 S vibrating blade microtome is designed to meet the highest sectioning demands for cutting fresh and fixed tissue in Neuroscience. To achieve sections of the highest quality that retain viable cells on the section surface, the vertical deflection of the blade can be measured by Leica's Vibrocheck measurement device and minimised with the blade holder. The instrument was designed in collaboration with Prof. Dr. Peter Jonas and his group at the Physiology Department, Freiburg Germany. [Product Link]

RRID:SCR_020243

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Tousimis Autosamdri 815B Critical Point Dryer

Critical point dryer with wafer holders that allow automatic anti stiction processing up to 5 pieces of either 100mm, 75mm, 50mm wafers or 10mm square die per process run. All internal surfaces are inert to CO2 and ultra pure alcohols. [Product Link]

RRID:SCR_018642

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Zeiss Sigma VP Scanning Electron Microscope

ZEISS SIGMA VP field emission scanning electron microscope (FE-SEM) for imaging of non-conducting samples. It images of bacteria, cells, plants and organisms. Uses ATLAS software and can be combined with 3View technology from Gatan Inc. [Product Link]

RRID:SCR_020928

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Services Provided:

Cell Imaging

Correlative Light Electron Microscopy

Electron Microscopy

Freeze Substitution

High-Pressure Freezing

Image Aquisition

Image Processing And Analysis

Immuno Electron Microscopy

Sample Preparation

Scanning Electron Microscopy

Sputter Coating

Staining

Tissue Processing

Training

Transmission Electron Microscopy

Relevant Publications:

1.) Vandael D, Vints K, Baatsen P, Śliwińska MA, Gabarre S, De Groef L, Moons L, Rybakin V, Gounko NV (2024 Jan 17). Cdk5-dependent rapid formation and stabilization of dendritic spines by corticotropin-releasing factor. Translational psychiatry, 14(1), 29. . ID: 38233378.


2.) Bademosi AT, Decet M, Kuenen S, Calatayud C, Swerts J, Gallego SF, Schoovaerts N, Karamanou S, Louros N, Martin E, Sibarita JB, Vints K, Gounko NV, Meunier FA, Economou A, Versées W, Rousseau F, Schymkowitz J, Soukup SF, Verstreken P (2023 May 3). EndophilinA-dependent coupling between activity-induced calcium influx and synaptic autophagy is disrupted by a Parkinson-risk mutation. Neuron, 111(9), 1402-1422.e13. . ID: 36827984.


3.) Iwata R, Casimir P, Erkol E, Boubakar L, Planque M, Gallego López IM, Ditkowska M, Gaspariunaite V, Beckers S, Remans D, Vints K, Vandekeere A, Poovathingal S, Bird M, Vlaeminck I, Creemers E, Wierda K, Corthout N, Vermeersch P, Carpentier S, Davie K, Mazzone M, Gounko NV, Aerts S, Ghesquière B, Fendt SM, Vanderhaeghen P (2023 Feb 10). Mitochondria metabolism sets the species-specific tempo of neuronal development. Science (New York, N.Y.), 379(6632), eabn4705. doi: 10.1126/science.abn4705. ID: 36705539.


4.) Rosier K, McDevitt MT, Smet J, Floyd BJ, Verschoore M, Marcaida MJ, Bingman CA, Lemmens I, Dal Peraro M, Tavernier J, Cravatt BF, Gounko NV, Vints K, Monnens Y, Bhalla K, Aerts L, Rashan EH, Vanlander AV, Van Coster R, Régal L, Pagliarini DJ, Creemers JWM (2021 Dec 17). Prolyl endopeptidase-like is a (thio)esterase involved in mitochondrial respiratory chain function. iScience, 24(12), 103460. . ID: 34888501.


5.) Jacquemyn J, Foroozandeh J, Vints K, Swerts J, Verstreken P, Gounko NV, Gallego SF, Goodchild R (2021 Sep 1). Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. The EMBO journal, 40(17), e106914. . ID: 34313336.


6.) Gabarre S, Vernaillen F, Baatsen P, Vints K, Cawthorne C, Boeynaems S, Michiels E, Vandael D, Gounko NV, Munck S (2021 Jul 30). A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography. BMC biology, 19(1), 152. . ID: 34330271.


7.) Vandael D, Wierda K, Vints K, Baatsen P, De Groef L, Moons L, Rybakin V, Gounko NV (2021 Jul 7). Corticotropin-releasing factor induces functional and structural synaptic remodelling in acute stress. Translational psychiatry, 11(1), 378. . ID: 34234103.


8.) Brouwers B, Coppola I, Vints K, Dislich B, Jouvet N, Van Lommel L, Segers C, Gounko NV, Thorrez L, Schuit F, Lichtenthaler SF, Estall JL, Declercq J, Ramos-Molina B, Creemers JWM (2021 Feb). Loss of Furin in β-Cells Induces an mTORC1-ATF4 Anabolic Pathway That Leads to β-Cell Dysfunction. Diabetes, 70(2), 492-503. doi: 10.2337/db20-0474. ID: 33277337.


9.) Vints K, Baatsen P, Gounko NV (2021). An accelerated procedure for approaching and imaging of optically branded region of interest in tissue. Methods in cell biology, 162(), 205-221. doi: 10.1016/bs.mcb.2020.08.002. ID: 33707013.


10.) Baatsen P, Gabarre S, Vints K, Wouters R, Vandael D, Goodchild R, Munck S, Gounko NV (2021). Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy. Frontiers in cell and developmental biology, 9(), 737621. . ID: 34977003.


11.) Apóstolo N, Smukowski SN, Vanderlinden J, Condomitti G, Rybakin V, Ten Bos J, Trobiani L, Portegies S, Vennekens KM, Gounko NV, Comoletti D, Wierda KD, Savas JN, de Wit J (2020 Oct 14). Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer. Nature communications, 11(1), 5171. . ID: 33057002.


12.) Vandael D, Gounko NV (2019 Oct 22). Corticotropin releasing factor-binding protein (CRF-BP) as a potential new therapeutic target in Alzheimer Translational psychiatry, 9(1), 272. . ID: 31641098.


13.) Vandoorne T, Veys K, Guo W, Sicart A, Vints K, Swijsen A, Moisse M, Eelen G, Gounko NV, Fumagalli L, Fazal R, Germeys C, Quaegebeur A, Fendt SM, Carmeliet P, Verfaillie C, Van Damme P, Ghesquière B, De Bock K, Van Den Bosch L (2019 Sep 12). Differentiation but not ALS mutations in FUS rewires motor neuron metabolism. Nature communications, 10(1), 4147. . ID: 31515480.


14.) Boey A, Rybakin V, Kalicharan D, Vints K, Gounko NV (2019 May). Gold-substituted Silver-intensified Peroxidase Immunolabeling for FIB-SEM Imaging. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 67(5), 351-360. . ID: 30624131.


15.) Vints K, Vandael D, Baatsen P, Pavie B, Vernaillen F, Corthout N, Rybakin V, Munck S, Gounko NV (2019 Jan 15). Modernization of Golgi staining techniques for high-resolution, 3-dimensional imaging of individual neurons. Scientific reports, 9(1), 130. . ID: 30644431.


16.) Spinazzi M, Radaelli E, Horré K, Arranz AM, Gounko NV, Agostinis P, Maia TM, Impens F, Morais VA, Lopez-Lluch G, Serneels L, Navas P, De Strooper B (2019 Jan 2). PARL deficiency in mouse causes Complex III defects, coenzyme Q depletion, and Leigh-like syndrome. Proceedings of the National Academy of Sciences of the United States of America, 116(1), 277-286. . ID: 30578322.


17.) Condomitti G, Wierda KD, Schroeder A, Rubio SE, Vennekens KM, Orlandi C, Martemyanov KA, Gounko NV, Savas JN, de Wit J (2018 Oct 10). An Input-Specific Orphan Receptor GPR158-HSPG Interaction Organizes Hippocampal Mossy Fiber-CA3 Synapses. Neuron, 100(1), 201-215.e9. . ID: 30290982.


18.) Lauwers E, Wang YC, Gallardo R, Van der Kant R, Michiels E, Swerts J, Baatsen P, Zaiter SS, McAlpine SR, Gounko NV, Rousseau F, Schymkowitz J, Verstreken P (2018 Sep 6). Hsp90 Mediates Membrane Deformation and Exosome Release. Molecular cell, 71(5), 689-702.e9. doi: 10.1016/j.molcel.2018.07.016. ID: 30193096.


19.) Schroeder A, Vanderlinden J, Vints K, Ribeiro LF, Vennekens KM, Gounko NV, Wierda KD, de Wit J (2018 Jul 25). A Modular Organization of LRR Protein-Mediated Synaptic Adhesion Defines Synapse Identity. Neuron, 99(2), 329-344.e7. doi: 10.1016/j.neuron.2018.06.026. ID: 29983322.


20.) Briz V, Restivo L, Pasciuto E, Juczewski K, Mercaldo V, Lo AC, Baatsen P, Gounko NV, Borreca A, Girardi T, Luca R, Nys J, Poorthuis RB, Mansvelder HD, Fisone G, Ammassari-Teule M, Arckens L, Krieger P, Meredith R, Bagni C (2017 Aug 17). The non-coding RNA BC1 regulates experience-dependent structural plasticity and learning. Nature communications, 8(1), 293. . ID: 28819097.


Description:

The Electron Microscopy Core Facility (EMCF) offers TEM of cells and tissues, negative staining, whole-mount SEM, correlative LM and EM, and immunogold labeling, vEM.