University of California, San Francisco
Eric Tse
https://emcore.ucsf.edu/
RRID: RRID:SCR_025781
1.) Pandey A, Rohweder PJ, Chan LM, Ongpipattanakul C, Chung DH, Paolella B, Quimby FM, Nguyen N, Verba KA, Evans MJ, Craik CS (2025 Jan 15). Therapeutic Targeting and Structural Characterization of a Sotorasib-Modified KRAS G12C-MHC I Complex Demonstrate the Antitumor Efficacy of Hapten-Based Strategies. Cancer research, 85(2), 329-341. . PMID: 39656104.
2.) Louder RK, Park G, Ye Z, Cha JS, Gardner AM, Lei Q, Ranjan A, Höllmüller E, Stengel F, Pugh BF, Wu C (2024 Nov 27). Molecular basis of global promoter sensing and nucleosome capture by the SWR1 chromatin remodeler. Cell, 187(24), 6849-6864.e18. . PMID: 39357520.
3.) Chen X, Wang L, Xie J, Nowak JS, Luo B, Zhang C, Jia G, Zou J, Huang D, Glatt S, Yang Y, Su Z (2024 Nov 15). RNA sample optimization for cryo-EM analysis. Nature protocols, (), . doi: 10.1038/s41596-024-01072-1. PMID: 39548288.
4.) Lu Y, Liang K, Zhan X (2024 Oct 16). Structure of a step II catalytically activated spliceosome from Chlamydomonas reinhardtii. The EMBO journal, (), . doi: 10.1038/s44318-024-00274-3. PMID: 39415054.
5.) Sanchez JC, Pierson J, Borcik CG, Rienstra CM, Wright ER (2024 Sep 19). High-Resolution Cryo-EM Structure Determination of α-synuclein - A Prototypical Amyloid Fibril. bioRxiv : the preprint server for biology, (), . . PMID: 39345396.
6.) Wagner J, Carvajal AI, Bracher A, Beck F, Wan W, Bohn S, Körner R, Baumeister W, Fernandez-Busnadiego R, Hartl FU (2024 Sep). Visualizing chaperonin function in situ by cryo-electron tomography. Nature, 633(8029), 459-464. . PMID: 39169181.
7.) Dederer V, Sanz Murillo M, Karasmanis EP, Hatch KS, Chatterjee D, Preuss F, Abdul Azeez KR, Nguyen LV, Galicia C, Dreier B, Plückthun A, Versees W, Mathea S, Leschziner AE, Reck-Peterson SL, Knapp S (2024 Jul). A designed ankyrin-repeat protein that targets Parkinson's disease-associated LRRK2. The Journal of biological chemistry, 300(7), 107469. . PMID: 38876305.
8.) Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, Harper JW (2024 Mar). Combinatorial selective ER-phagy remodels the ER during neurogenesis. Nature cell biology, 26(3), 378-392. . PMID: 38429475.
9.) Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR (2024 Feb). B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage. The EMBO journal, 43(4), 484-506. . PMID: 38177497.
10.) Law MCY, Zhang K, Tan YB, Nguyen TM, Luo D (2023 Dec). Chikungunya virus nonstructural protein 1 is a versatile RNA capping and decapping enzyme. The Journal of biological chemistry, 299(12), 105415. . PMID: 37918803.
11.) Valik JK, Ward L, Tanushi H, Johansson AF, Färnert A, Mogensen ML, Pickering BW, Herasevich V, Dalianis H, Henriksson A, Nauclér P (2023 Jul 20). Predicting sepsis onset using a machine learned causal probabilistic network algorithm based on electronic health records data. Scientific reports, 13(1), 11760. . PMID: 37474597.
12.) Das R, Haque MA, Sobi RA, Sultana AA, Khan MA, Gazi A, Mahfuz M, Nahar B, Bardhan PK, Ahmed T (2023 Jan 25). Citrulline and kynurenine to tryptophan ratio: potential EED (environmental enteric dysfunction) biomarkers in acute watery diarrhea among children in Bangladesh. Scientific reports, 13(1), 1416. . PMID: 36697429.
13.) Wang H, Hayer-Hartl M (2023). Phase Separation of Rubisco by the Folded SSUL Domains of CcmM in Beta-Carboxysome Biogenesis. Methods in molecular biology (Clifton, N.J.), 2563(), 269-296. doi: 10.1007/978-1-0716-2663-4_14. PMID: 36227479.
14.) Li S, Zhang K, Chiu W (2023). Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA. Methods in molecular biology (Clifton, N.J.), 2568(), 179-192. doi: 10.1007/978-1-0716-2687-0_12. PMID: 36227569.
15.) Zhang K, Julius D, Cheng Y (2022 Dec 16). A step-by-step protocol for capturing conformational snapshots of ligand gated ion channels by single-particle cryo-EM. STAR protocols, 3(4), 101732. . PMID: 36181684.
16.) Belinite M, Khusainov I, Marzi S (2022 Oct 20). Staphylococcus aureus 30S Ribosomal Subunit Purification and Its Biochemical and Cryo-EM Analysis. Bio-protocol, 12(20), . . PMID: 36353712.
17.) Tian B, Zhou M, Feng F, Xu X, Wang P, Luan H, Ji W, Xue Y, Xu T (2022 Aug 31). Cryogenic superresolution correlative light and electron microscopy of vitreous sections. Biophysics reports, 8(4), 193-204. . PMID: 37288007.
18.) Chmielewski D, Schmid MF, Simmons G, Jin J, Chiu W (2022 Aug). Chikungunya virus assembly and budding visualized in situ using cryogenic electron tomography. Nature microbiology, 7(8), 1270-1279. . PMID: 35773421.
19.) DiCecco LA, Berry S, Jonaid GM, Solares MJ, Kaylor L, Gray JL, Bator C, Dearnaley WJ, Spilman M, Dressel-Dukes MJ, Grandfield K, McDonald Esstman SM, Kelly DF (2022 Jul 20). Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice. Journal of visualized experiments : JoVE, (185), . doi: 10.3791/63856. PMID: 35938795.
20.) Cai S, Wu Y, Guillén-Samander A, Hancock-Cerutti W, Liu J, De Camilli P (2022 Jul 19). In situ architecture of the lipid transport protein VPS13C at ER-lysosome membrane contacts. Proceedings of the National Academy of Sciences of the United States of America, 119(29), e2203769119. . PMID: 35858323.
21.) Sheng Y, Morris K, Radecke J, Zhang P (2022 Jul 12). Cryo-electron Tomography Remote Data Collection and Subtomogram Averaging. Journal of visualized experiments : JoVE, (185), . . PMID: 35913165.
22.) Yu G, Bai Y, Zhang ZY (2022 Jun 17). Purification and cryo-EM structure determination of VCP/p97 dodecamers from mammalian and bacterial cells. STAR protocols, 3(2), 101339. . PMID: 35496809.
23.) Diwanji D, Trenker R, Jura N, Verba KA (2022). Efficient expression, purification, and visualization by cryo-EM of unliganded near full-length HER3. Methods in enzymology, 667(), 611-632. . PMID: 35525556.
24.) Klumpe S, Fung HK, Goetz SK, Zagoriy I, Hampoelz B, Zhang X, Erdmann PS, Baumbach J, Müller CW, Beck M, Plitzko JM, Mahamid J (2021 Dec 24). A modular platform for automated cryo-FIB workflows. eLife, 10(), . . PMID: 34951584.
25.) Swanson NA, Lokareddy RK, Li F, Hou CF, Pavlenok M, Niederweis M, Cingolani G (2021 Dec 17). Expression and purification of phage T7 ejection proteins for cryo-EM analysis. STAR protocols, 2(4), 100960. . PMID: 34825220.
26.) Martinez SE, Singh A, De Wijngaert B, Sultana S, Dharia C, Vanbuel H, Shen J, Vasilchuk D, Patel SS, Das K (2021 Jun 18). Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates. STAR protocols, 2(2), 100431. . PMID: 33870232.
27.) Ruijgrok PV, Ghosh RP, Zemsky S, Nakamura M, Gong R, Ning L, Chen R, Vachharajani VT, Chu AE, Anand N, Eguchi RR, Huang PS, Lin MZ, Alushin GM, Liphardt JT, Bryant Z (2021 May). Optical control of fast and processive engineered myosins in vitro and in living cells. Nature chemical biology, 17(5), 540-548. . PMID: 33603247.
28.) Chen X, Shi D, Zhang P, Walters KJ (2021 Mar 19). An optimized protocol for acquiring and processing cryo-EM data of human 26S proteasome with M1-Ub(6). STAR protocols, 2(1), 100278. . PMID: 33521680.
29.) Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC (2020 Aug 7). Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling. eLife, 9(), . . PMID: 32762848.
30.) Noreng S, Posert R, Bharadwaj A, Houser A, Baconguis I (2020 Jul 30). Molecular principles of assembly, activation, and inhibition in epithelial sodium channel. eLife, 9(), . . PMID: 32729833.
31.) Xue J, Xie T, Zeng W, Jiang Y, Bai XC (2020 Jul 29). Cryo-EM structures of human ZnT8 in both outward- and inward-facing conformations. eLife, 9(), . . PMID: 32723473.
32.) Echigoya K, Koyama M, Negishi L, Takizawa Y, Mizukami Y, Shimabayashi H, Kuroda A, Kurumizaka H (2020 Jul 16). Nucleosome binding by the pioneer transcription factor OCT4. Scientific reports, 10(1), 11832. . PMID: 32678275.
33.) Ahel J, Lehner A, Vogel A, Schleiffer A, Meinhart A, Haselbach D, Clausen T (2020 Jun 23). Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism. eLife, 9(), . . PMID: 32573437.
34.) Zhang D, Zhang Y, Ma J, Zhu C, Niu T, Chen W, Pang X, Zhai Y, Sun F (2020 Mar 31). Cryo-EM structures of S-OPA1 reveal its interactions with membrane and changes upon nucleotide binding. eLife, 9(), . . PMID: 32228866.
35.) Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J (2020 Jan 20). The kinesin-5 tail domain directly modulates the mechanochemical cycle of the motor domain for anti-parallel microtubule sliding. eLife, 9(), . . PMID: 31958056.
36.) Guerrero-Ferreira R, Taylor NM, Arteni AA, Kumari P, Mona D, Ringler P, Britschgi M, Lauer ME, Makky A, Verasdonck J, Riek R, Melki R, Meier BH, Böckmann A, Bousset L, Stahlberg H (2019 Dec 9). Two new polymorphic structures of human full-length alpha-synuclein fibrils solved by cryo-electron microscopy. eLife, 8(), . . PMID: 31815671.
Core includes instrumentation for structural biology with focus on cryo-electron microscopy. Used for imaging ranging from atomic resolution to whole cells, with emphasis on linking structural and dynamics information with biological functions.
