Lunenfeld-Tanenbaum Research Institute

Contact Info:

Louise Brown

600 University Avenue

Mount Sinai Hospital, Sinai Health

Toronto, ON M5G 1X5

https://nbcc.lunenfeld.ca/facilities/high-res-imaging

Grants and Identifiers:

RRID: RRID:SCR_025389

Instrumentation:

Services Provided:

Bioluminsecence Imaging

Brightfield Microscopy

Cell Imaging

Confocal Microscopy

Confocal/ Super Resolution Microscope

Differential Interference Contrast Microscopy

Fluorescence Lifetime Imaging

Live Cell Imaging

Microscopy

Optical Imaging

Phase Contrast Microscopy

Spinning Disk Confocal Imaging

Super-resolution Microscopy

TIRF Microscopy

Widefield Microscopy

Relevant Publications:

1.) Bourmoum M, Radulovich N, Sharma A, Tkach JM, Tsao MS, Pelletier L (2024). β-catenin mediates growth defects induced by centrosome loss in a subset of APC mutant colorectal cancer independently of p53. PloS one, 19(2), e0295030. . ID: 38324534.

2.) Aubry A, Pearson JD, Charish J, Yu T, Sivak JM, Xirodimas DP, Avet-Loiseau H, Corre J, Monnier PP, Bremner R (2023 Aug 29). Deneddylation of ribosomal proteins promotes synergy between MLN4924 and chemotherapy to elicit complete therapeutic responses. Cell reports, 42(8), 112925. doi: 10.1016/j.celrep.2023.112925. ID: 37552601.

3.) Hall EA, Kumar D, Prosser SL, Yeyati PL, Herranz-Pérez V, García-Verdugo JM, Rose L, McKie L, Dodd DO, Tennant PA, Megaw R, Murphy LC, Ferreira MF, Grimes G, Williams L, Quidwai T, Pelletier L, Reiter JF, Mill P (2023 Feb 15). Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis. eLife, 12(), . . ID: 36790165.

4.) Tkach JM, Philip R, Sharma A, Strecker J, Durocher D, Pelletier L (2022 Jun 27). Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number. eLife, 11(), . . ID: 35758262.

5.) Prosser SL, Tkach J, Gheiratmand L, Kim J, Raught B, Morrison CG, Pelletier L (2022 Apr). Aggresome assembly at the centrosome is driven by CP110-CEP97-CEP290 and centriolar satellites. Nature cell biology, 24(4), 483-496. . ID: 35411088.

6.) Lequyer J, Philip R, Sharma A, Hsu WH, Pelletier L (2022). A fast blind zero-shot denoiser. Nature machine intelligence, 4(11), 953-963. . ID: 36415333.

7.) Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, Goodrich DW, Bremner R (2021 Aug 9). Binary pan-cancer classes with distinct vulnerabilities defined by pro- or anti-cancer YAP/TEAD activity. Cancer cell, 39(8), 1115-1134.e12. . ID: 34270926.

8.) Park K, Li C, Tsiropoulou S, Gonçalves J, Kondratev C, Pelletier L, Blacque OE, Leroux MR (2021 Jun 7). CDKL kinase regulates the length of the ciliary proximal segment. Current biology : CB, 31(11), 2359-2373.e7. doi: 10.1016/j.cub.2021.03.068. ID: 33857430.

9.) Tsatskis Y, Rosenfeld R, Pearson JD, Boswell C, Qu Y, Kim K, Fabian L, Mohammad A, Wang X, Robson MI, Krchma K, Wu J, Gonçalves J, Hodzic D, Wu S, Potter D, Pelletier L, Dunham WH, Gingras AC, Sun Y, Meng J, Godt D, Schedl T, Ciruna B, Choi K, Perry JRB, Bremner R, Schirmer EC, Brill JA, Jurisicova A, McNeill H (2020 Aug). The NEMP family supports metazoan fertility and nuclear envelope stiffness. Science advances, 6(35), eabb4591. . ID: 32923640.

10.) Modzelewska K, Brown L, Culotti J, Moghal N (2020 Jul 23). Sensory regulated Wnt production from neurons helps make organ development robust to environmental changes in C. elegans. Development (Cambridge, England), 147(14), . pii: dev186080. doi: 10.1242/dev.186080. ID: 32586974.

11.) Gonçalves J, Sharma A, Coyaud É, Laurent EMN, Raught B, Pelletier L (2020 Jul 6). LUZP1 and the tumor suppressor EPLIN modulate actin stability to restrict primary cilia formation. The Journal of cell biology, 219(7), . . ID: 32496561.

12.) Liu Y, Kim J, Philip R, Sridhar V, Chandrashekhar M, Moffat J, van Breugel M, Pelletier L (2020 Apr 23). Direct interaction between CEP85 and STIL mediates PLK4-driven directed cell migration. Journal of cell science, 133(8), . . ID: 32107292.

Description:

The advanced imaging facility of the Network Biology Collaborative Centre (NBCC) covers all microscopy needs from your everyday workhorse to super-resolution. Directed by Laurence Pelletier, the facility is affiliated with the Nikon Centre of Excellence at the Lunenfeld-Tanenbaum Research Institute where users have access to the newest technologies in advanced microscopy. Our full-service offerings include consultation on experimental design, advice on appropriate systems, training and support for assisted and unassisted use, as well as help with data analysis and image generation for publications. Applications: Fluorescence and brightfield image acquisition Whole-slide scanning Dynamic live-cell imaging Super-resolution imaging