Lunenfeld-Tanenbaum Research Institute

Contact Info:

Monica Hasegan

600 University Avenue

Mount Sinai Hospital, Sinai Health

Toronto, ON M5G 1X5

https://nbcc.lunenfeld.ca/facilities/high-content-screening

Grants and Identifiers:

RRID: RRID:SCR_025391

Instrumentation:

Services Provided:

Cell Imaging

High-content Screening

Image Aquisition

Image Processing And Analysis

Live Cell Imaging

Relevant Publications:

1.) Sifri C, Hoeg L, Durocher D, Setiaputra D (2023 Aug 3). An AlphaFold2 map of the 53BP1 pathway identifies a direct SHLD3-RIF1 interaction critical for shieldin activity. EMBO reports, 24(8), e56834. . ID: 37306046.

2.) Zhao Y, Tabet D, Rubio Contreras D, Lao L, Kousholt AN, Weile J, Melo H, Hoeg L, Feng S, Coté AG, Lin ZY, Setiaputra D, Jonkers J, Gingras AC, Gómez Herreros F, Roth FP, Durocher D (2023 Aug 3). Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens. Molecular cell, 83(15), 2792-2809.e9. . ID: 37478847.

3.) Kondratyev M, Pesic A, Ketela T, Stickle N, Beswick C, Shalev Z, Marastoni S, Samadian S, Dvorkin-Gheva A, Sayad A, Bashkurov M, Boasquevisque P, Datti A, Pugh TJ, Virtanen C, Moffat J, Grénman RA, Koritzinsky M, Wouters BG (2023 May 18). Identification of acquired Notch3 dependency in metastatic Head and Neck Cancer. Communications biology, 6(1), 538. . ID: 37202533.

4.) Gallo D, Young JTF, Fourtounis J, Martino G, Álvarez-Quilón A, Bernier C, Duffy NM, Papp R, Roulston A, Stocco R, Szychowski J, Veloso A, Alam H, Baruah PS, Fortin AB, Bowlan J, Chaudhary N, Desjardins J, Dietrich E, Fournier S, Fugère-Desjardins C, Goullet de Rugy T, Leclaire ME, Liu B, Bhaskaran V, Mamane Y, Melo H, Nicolas O, Singhania A, Szilard RK, Tkáč J, Yin SY, Morris SJ, Zinda M, Marshall CG, Durocher D (2022 Apr). CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition. Nature, 604(7907), 749-756. . ID: 35444283.

5.) Labibi B, Bashkurov M, Christova T, Attisano L (2021 Sep 17). High-content imaging and analysis to quantify the nuclear to cytoplasmic ratio of TGFβ and hippo effectors in mammalian cells. STAR protocols, 2(3), 100632. . ID: 34258593.

6.) Labibi B, Bashkurov M, Wrana JL, Attisano L (2020 Aug 21). Modeling the Control of TGF-β/Smad Nuclear Accumulation by the Hippo Pathway Effectors, Taz/Yap. iScience, 23(8), 101416. . ID: 32798968.

7.) Gonçalves J, Sharma A, Coyaud É, Laurent EMN, Raught B, Pelletier L (2020 Jul 6). LUZP1 and the tumor suppressor EPLIN modulate actin stability to restrict primary cilia formation. The Journal of cell biology, 219(7), . . ID: 32496561.

8.) Moskal N, Riccio V, Bashkurov M, Taddese R, Datti A, Lewis PN, Angus McQuibban G (2020 Jan 3). ROCK inhibitors upregulate the neuroprotective Parkin-mediated mitophagy pathway. Nature communications, 11(1), 88. . ID: 31900402.

9.) Yeung T, Fung O, Bashkurov M, Khandani A, Subedar O, Wudwud A, Shaw P, Clarke B, Bartlett J, Rottapel R, Kapus A (2019 Nov 19). Avoidance of apoptotic death via a hyperploid salvage survival pathway after platinum treatment in high grade serous carcinoma cell line models. Oncotarget, 10(62), 6691-6712. . ID: 31803363.

10.) Gill MK, Christova T, Zhang YY, Gregorieff A, Zhang L, Narimatsu M, Song S, Xiong S, Couzens AL, Tong J, Krieger JR, Moran MF, Zlotta AR, van der Kwast TH, Gingras AC, Sicheri F, Wrana JL, Attisano L (2018 Aug 29). A feed forward loop enforces YAP/TAZ signaling during tumorigenesis. Nature communications, 9(1), 3510. . ID: 30158528.

Description:

The high-content screening facility of the Network Biology Collaborative Centre provides fast, reliable and high-content optical imaging and analysis of fixed and live cells: more data, more measurements for quicker discoveries. Our full-service offerings include assistance with selecting the appropriate imaging platform, developing customized imaging assays, training in imaging and data analysis and assistance with image/data preparation for presentations and publications. Applications: Multi-plate based imaging applications including: High-resolution cell characterization and sub-cellular localization using IN cell Analyzers 3D Spheroid and Organoid imaging using a Nikon A1R BioPipeline Live-cell analysis (confluency, growth tracking) using an Incucyte - imaging within an incubator