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Network Biology Collaborative Centre Flow Cytometry

Lunenfeld-Tanenbaum Research Institute

Contact Info:

Michael Parsons

600 University Avenue

Mount Sinai Hospital, Sinai Health

Toronto, ON M5G 1X5

https://nbcc.lunenfeld.ca

Grants and Identifiers:

RRID: RRID:SCR_025384

Instrumentation:

BD LSRFortessa Fortessa Flow Cytometer

BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]

RRID:SCR_019601

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BD LSRFortessa X-20 Fortessa Flow Cytometer

BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]

RRID:SCR_019600

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Beckman Coulter Astrios Cell Sorter

MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. The ergonomic design of Astrios makes it faster and easier to operate. All components requiring operator control are within easy reach. Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel facilitates... Read more [Product Link]

RRID:SCR_019615

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Beckman Coulter Gallios Flow Cytometer

Flow Cytometer provides efficient acquisition of up to 12 parameters to achieve accurate size measurement and high resolution and processing. [Product Link]

RRID:SCR_019639

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Thermo Fisher Attune Nxt Nxt

2020 Attune NxT Flow Cytometer makes multiparametric flow cytometry available to both new and experienced researchers. [Product Link]

RRID:SCR_019590

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Services Provided:

Flow Cytometric Analysis

Flow Cytometry

Flow Cytometry Data Analysis

Imaging Flow Cytometry

Multi-color Flow Cytometry

Relevant Publications:

1.) Cho T, Hoeg L, Setiaputra D, Durocher D (2024 Apr 17). NFATC2IP is a mediator of SUMO-dependent genome integrity. Genes & development, 38(5), 233-252. . ID: 38503515.


2.) Lü Y, Cho T, Mukherjee S, Suarez CF, Gonzalez-Foutel NS, Malik A, Martinez S, Dervovic D, Oh RH, Langille E, Al-Zahrani KN, Hoeg L, Lin ZY, Tsai R, Mbamalu G, Rotter V, Ashton-Prolla P, Moffat J, Chemes LB, Gingras AC, Oren M, Durocher D, Schramek D (2024 Apr 5). Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability. Molecular systems biology, (), . doi: 10.1038/s44320-024-00032-x. ID: 38580884.


3.) Harding J, Vintersten-Nagy K, Yang H, Tang JK, Shutova M, Jong ED, Lee JH, Massumi M, Oussenko T, Izadifar Z, Zhang P, Rogers IM, Wheeler MB, Lye SJ, Sung HK, Li C, Izadifar M, Nagy A (2024 Apr). Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts. Nature biomedical engineering, 8(4), 427-442. . ID: 37996616.


4.) Sussman RG, Mburu J, Steele M, Bang A, Friedman J, Goldman R, Kirby M, Rand ML, Blanchette VS, Pluthero FG, Williams S, Kahr WHA (2024 Mar). Constitutive hypercoagulability in pediatric sickle cell disease patients with hemoglobin SS genotype. Research and practice in thrombosis and haemostasis, 8(3), 102374. . ID: 38605827.


5.) Collignon E, Cho B, Furlan G, Fothergill-Robinson J, Martin SB, McClymont SA, Ross RL, Limbach PA, Ramalho-Santos M (2023 Sep). m(6)A RNA methylation orchestrates transcriptional dormancy during paused pluripotency. Nature cell biology, 25(9), 1279-1289. doi: 10.1038/s41556-023-01212-x. ID: 37696947.


6.) Aubry A, Pearson JD, Charish J, Yu T, Sivak JM, Xirodimas DP, Avet-Loiseau H, Corre J, Monnier PP, Bremner R (2023 Aug 29). Deneddylation of ribosomal proteins promotes synergy between MLN4924 and chemotherapy to elicit complete therapeutic responses. Cell reports, 42(8), 112925. doi: 10.1016/j.celrep.2023.112925. ID: 37552601.


7.) Gallo D, Young JTF, Fourtounis J, Martino G, Álvarez-Quilón A, Bernier C, Duffy NM, Papp R, Roulston A, Stocco R, Szychowski J, Veloso A, Alam H, Baruah PS, Fortin AB, Bowlan J, Chaudhary N, Desjardins J, Dietrich E, Fournier S, Fugère-Desjardins C, Goullet de Rugy T, Leclaire ME, Liu B, Bhaskaran V, Mamane Y, Melo H, Nicolas O, Singhania A, Szilard RK, Tkáč J, Yin SY, Morris SJ, Zinda M, Marshall CG, Durocher D (2022 Apr). CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition. Nature, 604(7907), 749-756. . ID: 35444283.


8.) Pearson JD, Bremner R (2021 Dec 17). Lentiviral-mediated ectopic expression of YAP and TAZ in YAP(off) cancer cell lines. STAR protocols, 2(4), 100870. . ID: 34632420.


9.) Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, Goodrich DW, Bremner R (2021 Aug 9). Binary pan-cancer classes with distinct vulnerabilities defined by pro- or anti-cancer YAP/TEAD activity. Cancer cell, 39(8), 1115-1134.e12. . ID: 34270926.


Description:

The flow cytometry facility of the Network Biology Collaborative Centre (NBCC) provides an extensive variety of state-of-the-art flow cytometry analyzers and cell sorters, with a specialty in image-based flow cytometry and cell sorting. Our full-service offerings include access to a full suite of analyzers and cell sorters, unassisted and full-service cell sorting. comprehensive training on theory and on instruments, consultation on experiment design, protocol development, data analysis, manuscript preparation and grant submission. Applications include: - image-based analysis powered by machine learning on the Amnis ImageStream - image-based cell sorting using the BD BACS Discover S8 - cell sorting for a variety of applications including multi-color sort for bulk culture, indexed cell sort into 96-well plates, cell cycle phase sort and primary tissue sorting for RNA or DNA analysis