Lunenfeld-Tanenbaum Research Institute
Contact Info:
Michael Parsons
600 University Avenue
Mount Sinai Hospital, Sinai Health
Toronto, ON M5G 1X5
Grants and Identifiers:
RRID: RRID:SCR_025384
Instrumentation:
Attune Nxt Nxt
2020 Attune NxT Flow Cytometer makes multiparametric flow cytometry available to both new and experienced researchers. [Product Link]
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BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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BD LSRFortessa X-20 Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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Beckman Coulter Astrios Cell Sorter
MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. The ergonomic design of Astrios makes it faster and easier to operate. All components requiring operator control are within easy reach. Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel facilitates... Read more [Product Link]
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Beckman Coulter Gallios Flow Cytometer
Flow Cytometer provides efficient acquisition of up to 12 parameters to achieve accurate size measurement and high resolution and processing. [Product Link]
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Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
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Fiber Optic Button Response System
Instrument that accurately gathers participant responses and verifies signals. The Celeritas Series response units are assembled using high-impact, chemical resistant, medical grade plastic. The response units include a tactile indicator to ensure correct finger placement during experiments and comfortably attach to the participant?s wrists. The units communicate button presses through fiber optic cabling which connects to a Fiber Optic Interface Console located in the control room through an available wave guide. The interface console provides real-time feedback of participant responses via LED indicators and includes a set of switches which can be used to make responses for the participant as needed. [Product Link]
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Leica TCS SPE
High resolution, compact and robust confocal that enables immunohistochemical colocalization analysis of florescent markers. Leica TCS SPE confocal point-scanning, spectral system for fluorescence imaging of live or fixed cells. [Product Link]
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Services Provided:
Relevant Publications:
Description:
The flow cytometry facility of the Network Biology Collaborative Centre (NBCC) provides an extensive variety of state-of-the-art flow cytometry analyzers and cell sorters, with a specialty in image-based flow cytometry and cell sorting. Our full-service offerings include access to a full suite of analyzers and cell sorters, unassisted and full-service cell sorting. comprehensive training on theory and on instruments, consultation on experiment design, protocol development, data analysis, manuscript preparation and grant submission. Applications include: - image-based analysis powered by machine learning on the Amnis ImageStream - image-based cell sorting using the BD BACS Discover S8 - cell sorting for a variety of applications including multi-color sort for bulk culture, indexed cell sort into 96-well plates, cell cycle phase sort and primary tissue sorting for RNA or DNA analysis
