Mayo Clinic in Arizona
Contact Info:
Ildefonso Silva Junior
5951 E Mayo Blvd
Phoenix, AZ 85054
https://www.mayo.edu/research/core-resources/flow-cytometry-confocal-microscopy-core/overview
Grants and Identifiers:
RRID: RRID:SCR_025364
Instrumentation:
BD Biosciences FACSAria III Cell Sorter
Instrument for cell sorting by BD Biosciences where positive air pressure forces sample cell through an optically gel-coupled cuvette . Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point.Fluorescence activated cell sorting instrument. Flow cytometer as high speed fixed alignment benchtop cell sorter. Enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at time. [Product Link]
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(Equipment No Longer Active At this Facility)
BD FACSDiscover S8
Cell Sorter, equipped with 5 lasers (355, 405, 488, 561 and 640 nm excitation wavelengths) and 78 channels which can measure the entire emission spectra of the fluorescent dyes, is the first spectral flow cytometer sorter with sort-capable image analysis. [Product Link]
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BD FACSymphony A3 Cell Analyzer
Fluorescence activated cell analyzing instrument by BD Biosciences. Allows for the simultaneous detection of up to 30 parameters and 28 colors. [Product Link]
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BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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(Equipment No Longer Active At this Facility)
BD LSRFortessa X-20 Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
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Sony MA900 Multi-Application Cell Sorter
MA900 system include patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that simplifies operation to make sorting less subjective and improve reliability. Provides up to 14 parameters from 4 lasers with possibility to choose from 4 excitation lasers—488 nm, 405 nm, 561 nm, and 638 nm—on two beam spots. Free-form PMTs enable detection of fluorescence signals from each beam spot, allowing detection of up to 12 fluorescence parameters and two scatter parameters. [Product Link]
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Services Provided:
Cell Sorting (Assisted & Unassisted)
Relevant Publications:
Description:
The Flow Cytometry Core provides comprehensive cell analysis and sorting services using state-of-the-art instrumentation. Our analysis platforms include the BD LSRFortessa, BD FACSymphony A3, and Cytek Aurora, supporting high-parameter immunophenotyping and advanced single-cell analysis. For cell sorting, we offer a 14-parameter, 4-laser Sony MA900 for high-speed, high-purity sorts, as well as a 16-parameter, 5-laser BD FACSAria dedicated to BSL-3 cell sorting. These platforms support a wide range of experimental needs, from basic workflows to complex, high-containment applications.
