University of Nebraska–Lincoln
Javier Seravalli
E157 Beadle Center
1901 Vine Street
Lincoln, NE 68588
https://redoxbiologycenter.unl.edu/spectroscopy
RRID: RRID:SCR_011011
Agilent 7700x ICP-MS
Discontinued. ICP-MS Instruments offer matrix tolerance and unmatched interference removal and deliver fast, accurate, and consistently reproducible results. [Product Link]
RRID:SCR_019448
Jasco J-815 Spectropolarimeter
Circular dichroism (CD) spectroscopy measures differences in the absorption of left-handed polarized light versus right-handed polarized light that arise due to structural asymmetry. The absence of regular structure results in zero CD intensity, while an ordered structure results in a spectrum which can contain both positive and negative signals. [Product Link]
RRID:SCR_020145
Microcal VPViewerTM Differential Scanning Calorimeter VP--DSC
Differential scanning calorimeter for study of samples in solution. It measures intramolecular stability of proteins, nucleic acids, oligomeric proteins, nucleic acid duplexes, lipid and detergent micellar system. [Product Link]
RRID:SCR_018594
SCIEX 4000 QTRAP Mass Spectrometer
Hybrid triple quadrupole linear ion trap mass spectrometer for drug discovery and development, metabolite identification, contaminant testing, proteomic applications, post translationally modified proteins discovery, protein identification, and biomarker validation. [Product Link]
RRID:SCR_018630
Cary-Agilent Spectrofluorimeter
Spectrofluorimeter with multiple use modalities.
HiTek-TgK Scientific Stopped Flow
rapid mixing UV/visible and fluorescence spectrophotometer
Microcal VP -ITC
Isothermal Calorimeter VP-ITC (1.43 mL cell)
Nanotemper Microscale Thermophoresis
Fluorescence MST
Sartorius Octet N1
Single Channel BLI
Biolayer Interferometry (BLI)
Circular Dichroism (CD)
Differential Scanning Calorimetry
Fluorescence Spectroscopy
Inductively Coupled Plasma Mass-Spectrometry
Intact Protein Mass Spectrometry
Isothermal Titration Calorimetry (ITC)
Mass Spectrometry - Small Molecules
MicroScale Thermophoresis (MST)
Targeted Metabolomics
1.) Munan S, Yoval-Sánchez B, Yao C, Seravalli J, Abdulaziz EN, Heacock ML, Ramesh A, Galkin A, Cracan V (2025 Sep 4). Biochemical characterization of a flavodiiron protein from bird parasite Histomonas meleagridis: superoxide as a reaction intermediate. The Journal of biological chemistry, (), 110685. doi: 10.1016/j.jbc.2025.110685. ID: 40914251.
2.) Mone K, Singh S, Abdullatif F, Sur M, Rasquinha MT, Seravalli J, Zinniel DK, Mukhopadhyay I, Barletta RG, Gebregiworgis T, Reddy J (2025 Jul 21). Immunization with Complete Freund Vaccines, 13(7), . . ID: 40733745.
3.) Beltran DG, Wan T, Seravalli J, Horová M, Jones C, Li S, Ma P, Becker DF, Mower JP, Yao Q, Pan Y, Yu H, Steyn AJC, Zhang L (2025 Jun 5). Aromatic Patch in WhiB-Like Transcription Factors Facilitates Primary Sigma Factor Interaction in Mycobacterium tuberculosis. bioRxiv : the preprint server for biology, (), . . ID: 40502120.
4.) Seravalli J (2024). ICP-MS Analysis of Iron from Biological Samples. Methods in molecular biology (Clifton, N.J.), 2839(), 31-41. doi: 10.1007/978-1-0716-4043-2_2. ID: 39008246.
5.) Mascarenhas R, Guha A, Li Z, Ruetz M, An S, Seravalli J, Banerjee R (2023 Nov 2). Cobalt-Sulfur Coordination Chemistry Drives B(12) Loading onto Methionine Synthase. Journal of the American Chemical Society, (), . . ID: 37916782.
6.) Nývltová E, Dietz JV, Seravalli J, Khalimonchuk O, Barrientos A (2022 Jun 24). Coordination of metal center biogenesis in human cytochrome c oxidase. Nature communications, 13(1), 3615. . ID: 35750769.
7.) Mao Y, Seravalli J, Smith TG, Morton M, Tanner JJ, Becker DF (2021 Nov 30). Evidence for Proline Catabolic Enzymes in the Metabolism of Thiazolidine Carboxylates. Biochemistry, 60(47), 3610-3620. . ID: 34752700.
8.) Pingault L, Palmer NA, Koch KG, Heng-Moss T, Bradshaw JD, Seravalli J, Twigg P, Louis J, Sarath G (2020 Oct 27). Differential Defense Responses of Upland and Lowland Switchgrass Cultivars to a Cereal Aphid Pest. International journal of molecular sciences, 21(21), . . ID: 33120946.
9.) Zhang B, Podolskiy DI, Mariotti M, Seravalli J, Gladyshev VN (2020 May). Systematic age-, organ-, and diet-associated ionome remodeling and the development of ionomic aging clocks. Aging cell, 19(5), e13119. . ID: 32323920.
Core provides instrumentation, training and support for any type of experimental work involving biophysical and spectroscopic measurements. The services that our core provides can be extended to partially purified proteins, whole cell assays and analysis of tissues.
