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UIUC, Roy J. Carver Biotechnology Center, Cytometry and Microscopy to Omics Facility

University of Illinois at Urbana-Champaign

Contact Info:

Mayandi Sivaguru

231 Edward R. Madigan Laboratory

1201 West Gregory Dr

Urbana, IL 61801

https://biotech.illinois.edu/cmto

Grants and Identifiers:

RRID: RRID:SCR_025272

Instrumentation:

Services Provided:

10x Genomics

10x Genomics Visium

10x Genomics Xenium

2D & 3D Illustration

3D & 4D Image Processing Stations

3D Visualization

Biological Mechanism

Biological photography/Photomicrography

Cell Sorting

Confocal Microscopy

Confocal/ Super Resolution Microscope

Experimental Design

Flow Cytometric Analysis

Flow Cytometry

Flow Cytometry Data Analysis

Fluorescence Microscope With Optical Sectioning Capability

Imaging Flow Cytometry

Immunofluorescence

Plate Reader

RNA Integrity

RNA Isolation

Sample Preparation

Scanning 20X Brightfield

Scanning 20X Flourescent

Scanning 40X Brightfield

Scanning 40X Fluorescent

Spatial Transcriptomics

Special Stains Histology

Staining

Super-resolution Microscopy

Surface Roughness

Training

Relevant Publications:

1.) Lumibao JC, Haak PL, Kolossov VL, Chen JE, Stutchman J, Ruiz A, Sivaguru M, Sarkaria JN, Harley BAC, Steelman AJ, Gaskins HR (2023 Nov). CHCHD2 mediates glioblastoma cell proliferation, mitochondrial metabolism, hypoxia‑induced invasion and therapeutic resistance. International journal of oncology, 63(5), . . ID: 37654190.


2.) Arcanjo RB, Vieira MC, Sivaguru M, Nowak RA (2023 Jan). Impact of mono(2-ethylhexyl) phthalate (MEHP) on the development of mouse embryo in vitro. Reproductive toxicology (Elmsford, N.Y.), 115(), 111-123. . ID: 36535558.


3.) Todorov LG, Sivaguru M, Krambeck AE, Lee MS, Lieske JC, Fouke BW (2022 Nov 1). GeoBioMed perspectives on kidney stone recurrence from the reactive surface area of SWL-derived particles. Scientific reports, 12(1), 18371. . ID: 36319741.


4.) Sivaguru M, Fouke BW (2022 Sep 29). Renal Macrophages and Multinucleated Giant Cells: Ferrymen of the River Styx? Kidney360, 3(9), 1616-1619. . ID: 36245644.


5.) Keenan-Jones D, Motta D, Garcia MH, Sivaguru M, Perillo M, Shosted RK, Fouke BW (2022 Jan 24). Travertine crystal growth ripples record the hydraulic history of ancient Rome Scientific reports, 12(1), 1239. . ID: 35075188.


6.) Sivaguru M, Saw JJ, Wilson EM, Lieske JC, Krambeck AE, Williams JC, Romero MF, Fouke KW, Curtis MW, Kear-Scott JL, Chia N, Fouke BW (2021 Jul). Human kidney stones: a natural record of universal biomineralization. Nature reviews. Urology, 18(7), 404-432. doi: 10.1038/s41585-021-00469-x. ID: 34031587.


7.) Sivaguru M, Todorov LG, Miller CAH, Fouke CE, Munro CMO, Fouke KW, Fouke KE, Baughman ME, Fouke BW (2021 Jan 26). Corals regulate the distribution and abundance of Symbiodiniaceae and biomolecules in response to changing water depth and sea surface temperature. Scientific reports, 11(1), 2230. . ID: 33500473.


8.) Sivaguru M, Lieske JC, Krambeck AE, Fouke BW (2020 Jan). GeoBioMed sheds new light on human kidney stone crystallization and dissolution. Nature reviews. Urology, 17(1), 1-2. doi: 10.1038/s41585-019-0256-5. ID: 31676884.


9.) Sivaguru M, Khaw YM, Inoue M (2019 Aug). A Confocal Reflection Super-Resolution Technique to Image Golgi-Cox Stained Neurons. Journal of microscopy, 275(2), 115-130. . ID: 31237354.


10.) Sivaguru M, Saw JJ, Williams JC Jr, Lieske JC, Krambeck AE, Romero MF, Chia N, Schwaderer AL, Alcalde RE, Bruce WJ, Wildman DE, Fried GA, Werth CJ, Reeder RJ, Yau PM, Sanford RA, Fouke BW (2018 Sep 13). Geobiology reveals how human kidney stones dissolve in vivo. Scientific reports, 8(1), 13731. . ID: 30213974.


Description:

The Cytometry and Microscopy to Omics (CMtO) Facility, couples cytometry with microscopy, genomics and proteomics using state-of-the-art instrumentation, end-to-end expertise and services. Maintaining our long-term commitment to core services in cytometry, we utilize advanced super resolution microscopy techniques to gain a significantly more in-depth understanding of cell sorting, analysis and enable cutting-edge spatial and single cell and multi-omics services for the UIUC campus and beyond.