University of Pittsburgh
James Conway
3501 Fifth Avenue
Pittsburgh, PA 15213
https://cryoem.structbio.pitt.edu
RRID: RRID:SCR_025216
FEI Aquilos 2 Cryo-FIB for Life Sciences
Sample preparation tool used for the preparation of thin, electron transparent lamellas for high resolution cryo electron tomography or MicroED of micro crystals. [Product Link]
RRID:SCR_019880
FEI Tecnai T12 electron microscope
Tecnai T12 microscope combines all imaging, diffraction, and analytical techniques at good spatial resolution and detection efficiency. Transmission electron microscope with operating voltage range of 20 to 120 kV. FEI Tecnai T12 120kV TEM and 2k TVIPS camera. [Product Link]
RRID:SCR_022981
Thermo Fisher Scientific Krios 3Gi
Cryo-electron microscope
Thermo Fisher Scientific TF20
Cryo-electron microscope
Cryo-Electron Microscopy Of Particulate Samples
Data Collection
Grid Preparation
1.) Zhang X, Guseinov AA, Jenkins L, Zhou J, Gossen F, Wang P, Al Mahmud Z, Li Y, Mahardhika AB, Muller CE, Feng M, Russell AJ, Tikhonova IG, Milligan G, Zhang C (2026 Apr 12). Mechanism of GPR84 allosteric modulation at a helix 8-proximate site. bioRxiv : the preprint server for biology, (), . . PMCID: 13082019.
2.) Johnson ME, Ozturk BE, Tugwell TH, Lambros M, Janowitz HN, Flohr K, Sedorovitz M, Campello L, Hartung JE, Hogle B, Gillespie M, Schriever H, Aweidah H, Koester J, Clausen I, Seeber S, Revelant F, Schreurs R, Koechl F, Sieving PA, Sahel JA, Stauffer WR, Peixoto RT, Fauser S, Lin R, Conway JF, da Silva S, Krol J, Betegon M, Byrne LC (2026 Mar 25). Structural dynamics insights into principles underlying the fitness of new broadly potent AAVs. bioRxiv : the preprint server for biology, (), . . PMCID: 13042077.
3.) Gluszko A, Kania D, Hong CS, Pietrowska M, Conway JF, Whiteside TL (2026 Mar). Nucleic Acids on the Surface and Lumen of Tumor-Derived Small Extracellular Vesicles as Potential Cancer Biomarkers. Cells, 15(6), . . PMCID: 13025544.
4.) Blauwkamp JA, Rajaram K, Staggers SR, Harrigan O, Doud EH, Xu W, Ke H, Prigge ST, Sun SY, Absalon S (2025 Nov 27). An essential adaptor for apicoplast fission and inheritance in malaria parasites Nature Communications, 16(), 11325. . PMCID: 12722261.
5.) Belford AK, Maurer JB, Duda RL, Huet A, Conway JF (2025 Nov 23). Structural insights into scaffold-guided assembly of the Pseudomonas phage D3 capsid Nature Communications, 16(), 11586. . PMCID: 12749128.
6.) Hansen KH, Golcuk M, Byeon CH, Tunc A, Plechinger EB, Dueholm MKD, Conway JF, Andreasen M, Gur M, Akbey Ü (2025 Sep 26). Structural basis of Pseudomonas biofilm-forming functional amyloid FapC formation. Science advances, 11(39), eadx7829. . PMID: 40991694.
7.) Wang P, Zhang X, Guseinov AA, Jenkins L, von Hallerstein C, Colburn JD, Ives R, Luscombe VB, Marsango S, Oktavia L, Raja A, Greaves DR, Biggin PC, Milligan G, Zhang C, Tikhonova IG, Russell AJ (2025 Aug 2). Steric control of signaling bias in the immunometabolic receptor GPR84 bioRxiv, (), 2025.07.30.667614. . PMCID: 12324463.
8.) Fischer HL, Kline C, Duprex WP, McCarthy KR, Watkins SC, Conway JF, Ambrose Z (2025 May 23). Deletion of the Envelope gene attenuates SARS-CoV-2 infection by altered Spike localization and increased cell-to-cell transmission bioRxiv, (), 2025.05.20.655126. . PMCID: 12140015.
9.) Huang H, Mu Y, Huang Y, Ji B, Wang Y, Chen CY, Chen Y, Luo Z, Li S, Zhang Z, Wang L, Conway JF, Yang D, Wang J, Sun J, Li S (2025 May 5). Rational development of gemcitabine-based nanoplatform for targeting SERPINB9/Granzyme B axis to overcome chemo-immune-resistance Nature Communications, 16(), 4176. . PMCID: 12053578.
10.) Blauwkamp J, Rajaram K, Staggers SR, Harrigan O, Doud EH, Prigge ST, Sun SY, Absalon S (2025 May 5). An Essential Adaptor for Apicoplast Fission and Inheritance in Malaria Parasites Research Square, (), rs.3.rs-6457426. . PMCID: 12083691.
11.) Wieteska Ł, Taylor AB, Punch E, Coleman JA, Conway IO, Lin YF, Byeon CH, Hinck CS, Krzysiak T, Ishima R, López-Casillas F, Cherepanov P, Bernard DJ, Hill CS, Hinck AP (2025 Feb 26). Structures of TGF-β with betaglycan and signaling receptors reveal mechanisms of complex assembly and signaling Nature Communications, 16(), 1778. . PMCID: 11865472.
12.) Vetturini AJ, Cagan J, Taylor RE (2024 Dec 31). Generative design-enabled exploration of wireframe DNA origami nanostructures Nucleic Acids Research, 53(2), gkae1268. . PMCID: 11754647.
The Cryo-electron Microscopy Facility provides access to cutting edge instrumentation for high resolution structural analysis of proteins, protein complexes, viruses, macromolecular assemblies, cellular organelles, and bacterial cells.
