University of Pittsburgh
Denise Prosser
5117 Centre Avenue
Research Pavilion, 1.18
Pittsburgh, PA 15213
https://hillmanresearch.upmc.edu/research/facilities/cancer-proteomics/luminex-core-laboratory/
RRID: RRID:SCR_025134
Assays and Measurements
Cytokine Assays
Data Analysis
Immunoassay
Luminex Bead Based Cytokine Assays
Luminex Services
Proteomics
Proteomics Analysis
Sample Preparation
1.) Hamsanathan S, Anthonymuthu T, Prosser D, Lokshin A, Greenspan SL, Resnick NM, Perera S, Okawa S, Narasimhan G, Gurkar AU (2024 Mar 7). A molecular index for biological age identified from the metabolome and senescence-associated secretome in humans. Aging cell, (), e14104. doi: 10.1111/acel.14104. ID: 38454639.
2.) Young Han C, Bedia JS, Yang WL, Hawley SJ, Bergan L, Hopper M, Celestino J, Guo J, Gornet TG, Soosaipillai A, Yang H, Doskocil SD, Lokshin AE, Handy BC, Diamandis EP, Moore RG, Lu KH, Lu Z, Anderson KS, Drescher CW, Skates SJ, Bast RC Jr (2024 Mar). Autoantibodies, antigen-autoantibody complexes and antigens complement CA125 for early detection of ovarian cancer. British journal of cancer, 130(5), 861-868. . ID: 38195887.
3.) Thomas CE, Adibi JJ, Kuipers AL, Diergaarde B, Luu HN, Jin A, Koh WP, Gao YT, Adams-Haduch J, Wang R, Lokshin A, Behari J, Yuan JM (2023 Jun). Soluble CD137 and risk of hepatocellular carcinoma: nested case-control studies in cohorts in Shanghai and Singapore. British journal of cancer, 128(11), 2081-2088. . ID: 36977826.
4.) Shaw R, Lokshin AE, Miller MC, Messerlian-Lambert G, Moore RG (2022 Mar 2). Stacking Machine Learning Algorithms for Biomarker-Based Preoperative Diagnosis of a Pelvic Mass. Cancers, 14(5), . . ID: 35267599.
5.) Berardi G, Frey-Law L, Sluka KA, Bayman EO, Coffey CS, Ecklund D, Vance CGT, Dailey DL, Burns J, Buvanendran A, McCarthy RJ, Jacobs J, Zhou XJ, Wixson R, Balach T, Brummett CM, Clauw D, Colquhoun D, Harte SE, Harris RE, Williams DA, Chang AC, Waljee J, Fisch KM, Jepsen K, Laurent LC, Olivier M, Langefeld CD, Howard TD, Fiehn O, Jacobs JM, Dakup P, Qian WJ, Swensen AC, Lokshin A, Lindquist M, Caffo BS, Crainiceanu C, Zeger S, Kahn A, Wager T, Taub M, Ford J, Sutherland SP, Wandner LD (2022). Multi-Site Observational Study to Assess Biomarkers for Susceptibility or Resilience to Chronic Pain: The Acute to Chronic Pain Signatures (A2CPS) Study Protocol. Frontiers in medicine, 9(), 849214. . ID: 35547202.
6.) Lokshin A, Bast RC, Rodland K (2021 Feb 15). Circulating Cancer Biomarkers. Cancers, 13(4), . . ID: 33671847.
7.) Tarney CM, Wang G, Bateman NW, Conrads KA, Zhou M, Hood BL, Loffredo J, Tian C, Darcy KM, Hamilton CA, Casablanca Y, Lokshin A, Conrads TP, Maxwell GL (2019 Nov). Biomarker panel for early detection of endometrial cancer in the Prostate, Lung, Colorectal, and Ovarian cancer screening trial. American journal of obstetrics and gynecology, 221(5), 472.e1-472.e10. doi: 10.1016/j.ajog.2019.06.005. ID: 31279844.
8.) Shurin GV, Kruglov O, Ding F, Lin Y, Hao X, Keskinov AA, You Z, Lokshin AE, LaFramboise WA, Falo LD Jr, Shurin MR, Bunimovich YL (2019 May 15). Melanoma-Induced Reprogramming of Schwann Cell Signaling Aids Tumor Growth. Cancer research, 79(10), 2736-2747. . ID: 30914431.
9.) Guo J, Yang WL, Pak D, Celestino J, Lu KH, Ning J, Lokshin AE, Cheng Z, Lu Z, Bast RC Jr (2019 Apr 28). Osteopontin, Macrophage Migration Inhibitory Factor and Anti-Interleukin-8 Autoantibodies Complement CA125 for Detection of Early Stage Ovarian Cancer. Cancers, 11(5), . . ID: 31035430.
10.) Gutkin DW, Shurin MR, El Azher MA, Shurin GV, Velikokhatnaya L, Prosser D, Shin N, Modugno F, Stemmer P, Elishaev E, Lokshin A (2019). Novel protein and immune response markers of human serous tubal intraepithelial carcinoma of the ovary. Cancer biomarkers : section A of Disease markers, 26(4), 471-479. . ID: 31658047.
11.) Linkov F, Goughnour SL, Adambekov S, Lokshin A, Kelley JL, Sukumvanich P, Comerci JT, Marra KG, Kokai LE, Rubin JP, Vlad AM, Philips BJ, Edwards RP (2018 Sep). Inflammatory biomarker in adipose stem cells of women with endometrial cancer. Biomarkers in medicine, 12(9), 945-952. . ID: 30043637.
12.) Al Efishat MA, Attiyeh MA, Eaton AA, Gönen M, Prosser D, Lokshin AE, Castillo CF, Lillemoe KD, Ferrone CR, Pergolini I, Mino-Kenudson M, Rezaee N, Dal Molin M, Weiss MJ, Cameron JL, Hruban RH, D (2018 Aug). Multi-institutional Validation Study of Pancreatic Cyst Fluid Protein Analysis for Prediction of High-risk Intraductal Papillary Mucinous Neoplasms of the Pancreas. Annals of surgery, 268(2), 340-347. . ID: 28700444.
13.) Linkov F, Goughnour SL, Ma T, Xu Z, Edwards RP, Lokshin AE, Ramanathan RC, Hamad GG, McCloskey C, Bovbjerg DH (2017 Oct). Changes in inflammatory endometrial cancer risk biomarkers in individuals undergoing surgical weight loss. Gynecologic oncology, 147(1), 133-138. . ID: 28797697.
14.) Linkov F, Goughnour SL, Edwards RP, Lokshin A, Ramanathan RC, Hamad GG, McCloskey C, Bovbjerg DH (2017 May). Endometrial cancer associated biomarkers in bariatric surgery candidates: exploration of racial differences. Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery, 13(5), 862-868. . ID: 28256392.
15.) Shurin MR, Ma Y, Keskinov AA, Zhao R, Lokshin A, Agassandian M, Shurin GV (2016 Sep 1). BAFF and APRIL from Activin A-Treated Dendritic Cells Upregulate the Antitumor Efficacy of Dendritic Cells In Vivo. Cancer research, 76(17), 4959-69. . ID: 27364554.
Our facility utilizes the LabMAP™ system developed by Luminex Corporation. Luminex technology is a flexible, easy-to-use protein analysis system that permits the simultaneous quantitative analysis of up to 100 different proteins, peptides, or RNA molecules in a single microtiter well. We use all commercially available assays. Millipore, R&D Systems, Bio-Rad, and Thermofisher offer a robust network of assays for many different species and sample types. The useful nature of being able to multiplex and customize markers enables advanced detection of useful biomarkers.
