University of Wisconsin Carbone Cancer Center
Contact Info:
1111 Highland Avenue
7016 WIMR
Madison, WI 53705
https://cancer.wisc.edu/research/resources/flow/
Grants and Identifiers:
RRID: RRID:SCR_025085
Other Citation Identifiers1S10RR025483-01
5P30CA014520-38
Instrumentation:
Attune Nxt Nxt
2020 Attune NxT Flow Cytometer makes multiparametric flow cytometry available to both new and experienced researchers. [Product Link]
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BD Biosciences FACSAria III Cell Sorter
Instrument for cell sorting by BD Biosciences where positive air pressure forces sample cell through an optically gel-coupled cuvette . Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point.Fluorescence activated cell sorting instrument. Flow cytometer as high speed fixed alignment benchtop cell sorter. Enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at time. [Product Link]
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BD FACSDiscover S8
Cell Sorter, equipped with 5 lasers (355, 405, 488, 561 and 640 nm excitation wavelengths) and 78 channels which can measure the entire emission spectra of the fluorescent dyes, is the first spectral flow cytometer sorter with sort-capable image analysis. [Product Link]
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BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
Citation IDs: 1S10OD018202-01
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Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
Citation IDs: 1S10OD025225-011S10OD025225-01
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Invitrogen Attune CytPix Flow Cytometer
Attune CytPix Flow Cytometer combines acoustic focusing flow cytometry with a high-speed camera to simultaneously collect fluorescent data and high-resolution brightfield images for each cell. It is used for automated, AI-driven morphological analysis alongside traditional immunophenotyping, allowing users to validate cell populations, identify cell-to-cell interactions, and verify cell health (e.g., apoptosis, contamination) in real-time. Attune CytPix is a benchtop analysis cytometer with 4 lasers (488, 405, 561, and 633nm) and a brightfield camera. [Product Link]
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Life Technologies Countess Automated Cell Counter
Automated cell counter that can perform cell counts, viability calculations, hemocytometry, fluorescent protein expression, and measure fluorescence capabilities. There are also reusable slides, fluorescence capabilities, and brightfield fluorescence channels. [Product Link]
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(Equipment No Longer Active At this Facility)
Luminex Amnis Image Stream Flow Cytometer
ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. [Product Link]
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Sony MA900 Multi-Application Cell Sorter
MA900 system include patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that simplifies operation to make sorting less subjective and improve reliability. Provides up to 14 parameters from 4 lasers with possibility to choose from 4 excitation lasers—488 nm, 405 nm, 561 nm, and 638 nm—on two beam spots. Free-form PMTs enable detection of fluorescence signals from each beam spot, allowing detection of up to 12 fluorescence parameters and two scatter parameters. [Product Link]
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Services Provided:
High Dimensional Flow Cytometry Analysis
Multiplexed Immunofluorescence
Shared Instrumentation Oversight & Maintenance
Single Particle Extracellular Vesicle Flow Cytometry
Relevant Publications:
Description:
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology, and treatment in basic, translational, and clinical research projects. The Flow Lab was established in 1988 as a resource for the University of Wisconsin–Madison campus. The facility maintains two laboratories: the main facility located in the Wisconsin Institutes for Medical Research (WIMR) a full service satellite facility in the UW Biotechnology Center.
