University of South Carolina
Austin Worden
6311 Garners Ferry Rd
Columbia, SC 29209
https://sc.edu/study/colleges_schools/medicine/research/research_facilities/instrumentation_resource_facility/index.php
RRID: RRID:SCR_024955
(Equipment No Longer Active At this Facility)
Leica Autostainer XL Automated Slide Stainer
Autostainer XL continues to provide reproducible, consistent, high-quality staining and increased workload throughput. Plus, combine it with the CV5030 Glass Coverslipper to create a workstation that eliminates the manual handling of slide racks between staining and coverslipping. Can be used stand-alone or integrated with the Leica CV5030 Glass Coverslipper to create a ‘walk-away, staining/coverslipping workstation. Upgrade at anytime to match your laboratory's specific needs. [Product Link]
RRID:SCR_020212
(Equipment No Longer Active At this Facility)
Microm HM505E Cryostat
Microtome cryostat with open top working area that can cut temperature to negative 35 degrees Celsius. It is equipped with automatic 24 hour defroster with interrupt key and handwheel brake. [Product Link]
RRID:SCR_018595
(Equipment No Longer Active At this Facility)
NanoPhotometer Pearl
(Equipment No Longer Active At this Facility)
QIAgility
(Equipment No Longer Active At this Facility)
QIAsymphony System
Agilent BioTek 50TS Microplate Washer
(Equipment No Longer Active At this Facility)
BD FACS Discover S8
BD FACS Discover S8 Cell Sorter
BD FACS Symphony A5 Cell Analyzer
(Equipment No Longer Active At this Facility)
BD FACS Symphony A5 Cell Analyzer
(Equipment No Longer Active At this Facility)
BioRad BioPlex 100 and 200 Systems
(Equipment No Longer Active At this Facility)
BioRad CFX Connect rtPCR
BioRad CFX96 Touch Real Time PRC
BioRad GelDoc XR+ System & iMark Microplate Absorbance Reader
(Equipment No Longer Active At this Facility)
BioRad GelDoc XR+ System and iMark Microplate Absorbance Reader
(Equipment No Longer Active At this Facility)
BioRad iCycler iQ rtPCR
Cressington 108 Sputter Coater
(Equipment No Longer Active At this Facility)
Dako Autostainer
Discover Echo Revolution Microscope
(Equipment No Longer Active At this Facility)
Discover Echo Revolve Microscope
(Equipment No Longer Active At this Facility)
Invitrogen EVOS FL Auto Cell Imaging System
Invitrogen EVOS FL Auto Cell Imaging System
JEOL 1400 Plus Transmission Electron Microscope
(Equipment No Longer Active At this Facility)
JEOL 1400 Plus Transmission Electron Microscope
(Equipment No Longer Active At this Facility)
JEOL JSM IT100
JEOL JSM IT100 Scanning Electron Microscope
(Equipment No Longer Active At this Facility)
JL Shepherd and Associates Model 143-68 Blood Product Irradiator
Lab Vision PT Module for Antigen Retrieval System
Leica ASP300 Tissue Processor
Leica CV 5000 Automatic Coverslipper
Leica RM2255 Automatic Microtome
(Equipment No Longer Active At this Facility)
Leica SP8 Multiphoton Confocal
Leica Stellaris 5 Confocal Microscope
(Equipment No Longer Active At this Facility)
Leica Stellaris 5 DIchroic
Leica Stellaris 8 FALCON with TauSTED System
Leica UC Enuity Ultramicrotome
(Equipment No Longer Active At this Facility)
Leica Ultracut R Ultramicrotome
Leica Ultracut R Ultramicrotome
(Equipment No Longer Active At this Facility)
Leica VT1200 S
Vibratome
(Equipment No Longer Active At this Facility)
Leica VT1200S Vibratome
(Equipment No Longer Active At this Facility)
LiCor Odyssey CLX System
Logos Biosystems X-Clarity Tissue Clearing System
(Equipment No Longer Active At this Facility)
Logos Biosystems X-Clarity Tissue Clearing System
(Equipment No Longer Active At this Facility)
Mauna Kea Cellvizio In Vivo Confocal
Nikon E600 Widefield Epifluorescence & Darkfield Microscopy System
(Equipment No Longer Active At this Facility)
Nikon E600 Widefield Epifluorescence and Darkfield Microscopy System
Nikon SMZ1500 Stereomicroscope
(Equipment No Longer Active At this Facility)
PerkinElmer IVIS Spectrum Fluorescence and Bioluminescence Imaging System
(Equipment No Longer Active At this Facility)
PerkinElmer Quantum GX microCT Imaging System
(Equipment No Longer Active At this Facility)
Polaron Sputter Coater
(Equipment No Longer Active At this Facility)
Qiagen Rotor-Gene Q rtPCR
(Equipment No Longer Active At this Facility)
Reichert U3 Ultramicrotome
Reichert-Jung 2030 Paraffin Rotary Microtome
(Equipment No Longer Active At this Facility)
Reichert-Jung 2030 Paraffin Rotary Microtomes
Revvity IVIS Spectrum Fluorescence and Bioluminescence Imaging System
Revvity Quantum GX microCT Imaging System
Tanner Scientific TN1500 Embedding Center
(Equipment No Longer Active At this Facility)
TECAN Freedom EVO System
ThermoFisher Scientific HM525
Cryostat
ThermoFisher Scientific iBright CL1500
ThermoFisher Scientific Nanodrop One
ThermoFisher Scientific QuantStudio 3 Real Time PCR
ThermoFisher Scientific QuantStudio Absolute Q Digital PCR System
ThermoFisher Scientific SimpliAmp Thermal Cycler
(Equipment No Longer Active At this Facility)
Tousimis Samdri PVT-3B Critical Point Dryer
Tousimis Samdri-PVT-3B Critical Point Dryer
Triangle Biomedical Sciences Embedding Center
(Equipment No Longer Active At this Facility)
Visual Sonics VEVO 3100 High Resolution In Vivo Imaging System
VisualSonics VEVO 3100 High Resolution In Vivo Imaging System
(Equipment No Longer Active At this Facility)
Zeiss Microm 505HN Cryostat
(Equipment No Longer Active At this Facility)
Zeiss SteREO Lumar V.12 Stereomicroscope
3D Bioprinting
Advanced Microscopy Applications: Confocal, Super Resolution, Slide Scanning, Microdisection, Lightsheet, STEM And FIB Microscopy
Biofabrication
Brightfield Microscopy
Clinical Imaging - Small Animal (luminescence/fluorescence)
Clinical Imaging - Small Animal (X-ray, Ultrasound, MicroCAT, MRI)
FACS Cell Sorting
Flow Cytometry
Histology
Histology Stains
Molecular Dynamics
Processing & Embedding- Histology
Scanning Electron Microscopy
Transmission Electron Microscopy
Widefield Microscopy
1.) Deloach S, Kumar A, Ruggiero E, Ball R, Gleason K, Kubinak J, DiPette DJ, Potts JD (2025 Oct 11). A Novel Peptoid Hybrid of Alpha-Calcitonin Gene-Related Peptide (α-CGRP) Ameliorates Cardiac Remodeling in Pressure Overload-Induced Heart Failure Cells, 14(20), 1580. . PMCID: 12564457.
2.) Cardaci TD, Bastian AV, Roark K, Bullard BM, NeSmith MM, Unger CA, Price RL, Kubinak JL, Murphy EA, VanderVeen BN (2025 Sep 10). Pro-Fibrotic Immune Cells and Fibro-Adipogenic Progenitors Contribute to Skeletal Muscle Extracellular Matrix Remodeling and Fibrosis in Cancer Cachexia American journal of physiology. Cell physiology, 329(4), C1283-C1299. . PMCID: 12516515.
3.) Nguyen V, Mao I, He S, Castellanos I, Azuero M, Hochman ML, Rong Y, Pernomian L, Chen EH, Friedman HI, Chen YH, Lu Q, Fan D, Wenceslau CF, Chen DB, Nelson JS, Jegga AG, Wang Y, Tan W (2025 Sep 7). Single-vessel transcriptome map pathological landscapes and reveal NR2F2-mediated smooth muscle cell phenotype acquisition in capillary malformations bioRxiv, (), 2025.09.02.673874. . PMCID: 12424823.
4.) Pernomian L, Waigi EW, Nguyen V, Mohammed AD, Costa TJ, Fontes MT, Kubinak JL, Aitken AV, Campana Biancardi V, Gleason K, Shazly T, Sinclair DA, McCarthy CG, Wang Y, Tan W, Ferreira Wenceslau C (2025 Sep 3). Single-Short Partial Reprogramming of the Endothelial Cells Decreases Blood Pressure via Attenuation of EndMT in Hypertensive Mice Circulation research, 137(8), 1092-1113. . PMCID: 12530109.
5.) Frick MA, Woodruff JL, Caudillo YM, Pikel KE, Rehm JV, Maciejewska N, Grillo CA, Reagan LP, Fadel JR (2025 Jul 29). Orexin/Hypocretin Modulates Neuroinflammatory Response to LPS in a Sex and Brain‐Region Specific Manner in Young Rats Journal of Neurochemistry, 169(8), e70175. . PMCID: 12305754.
6.) Azeredo PD, Fix C, Pernomian L, Wenceslau CF, Piroli GG, Vicente CP, Carver WE (2025 Jun 21). Tetrahydrocurcumin Outperforms Curcumin in Preventing Oxidative Stress-Induced Dysfunction in Tert-Butyl Hydroperoxide-Stimulated Cardiac Fibroblasts International Journal of Molecular Sciences, 26(13), 5964. . PMCID: 12250096.
7.) Amurrio E, Patel JH, Danaher M, Goodwin M, Kargbo P, Klimentova E, Lin S, Kelly MP (2025 Jun 13). Age-Related Increases in PDE11A4 Protein Expression Trigger Liquid–Liquid Phase Separation (LLPS) of the Enzyme That Can Be Reversed by PDE11A4 Small Molecule Inhibitors Cells, 14(12), 897. . PMCID: 12190369.
8.) Worden AN, Pittard EG, Stern M, Uline MJ, Potts JD (2025 Mar 17). The Role of the CXCL12/CXCR4 Signaling Pathway in Regulating Cellular Migration. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 31(2), . pii: ozaf011. doi: 10.1093/mam/ozaf011. PMID: 40095909.
9.) Waigi EW, Pernomian L, Crockett AM, Costa TJ, Townsend P Jr, Webb RC, McQuail JA, McCarthy CG, Hollis F, Wenceslau CF (2024 Jun 11). Vascular dysfunction occurs prior to the onset of amyloid pathology and Aβ plaque deposits colocalize with endothelial cells in the hippocampus of female APPswe/PSEN1dE9 mice GeroScience, 46(6), 5517-5536. . PMCID: 11493946.
10.) Pernomian L, Waigi EW, Nguyen V, Mohammed AD, da Costa TJ, Fontes MT, Kubinak JL, Aitken A, Biancardi VC, Sinclair DA, McCarthy CG, Wang Y, Tan W, Wenceslau CF (2024 May 21). A Single-Short Partial Reprogramming of the Endothelial Cells decreases Blood Pressure via attenuation of EndMT in Hypertensive Mice. bioRxiv : the preprint server for biology, (), . . PMID: 38826452.
Mission: By providing state-of-the-art biomedical instrumentation, the IRF seeks to facilitate the expansion of scientific knowledge and discovery in the USC School of Medicine Columbia and main campus research environments.
Purpose: As an integral component of the research and teaching mission of the University of South Carolina School of Medicine, the IRF houses several major pieces of state-of-the-art biotechnological equipment that provide investigation ranging from single cell and molecular level analysis to whole animal imaging. Additionally, the IRF maintains a full range of ancillary equipment available for sample preparation, image enhancement, and data analysis.
