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Research Core Facility

Louisiana State University Health Sciences Center Shreveport

Contact Info:

Paula Polk

1501 Kings Hwy

Shreveport, LA 71103

https://research.lsuhs.edu/cores/research-core-facilities

Grants and Identifiers:

RRID: RRID:SCR_024775

Instrumentation:

(Equipment No Longer Active At this Facility)

Leica TCS SP5 Broadband Confocal Laser Scanning Microscope

Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]

RRID:SCR_020233

cite this instrument

Services Provided:

10x Genomics

Cell Sorting

Confocal/ Super Resolution Microscope

Flow Cytometry

High Performance Computing

Mass Spectrometry

Metabolic Cages

Real-time qPCR

RNA Integrity

Sequencing - Next Generation Sequencing (NGS)

Transmission Electron Microscopy

Relevant Publications:

1.) Kaur H, Pandey N, Chandaluri L, Shaaban N, Kumar D, Pandit R, Martinez A, Anand SK, Das S, Rohilla S, Arias F, Reece E, Gaddam RR, Murnane KS, Vikram A, Mohandas R, Zhang X, Bhuiyan MA, Lu XH, Orr AW, Rom O, Yurdagul A Jr, Dhanesha N (2025 Jul 31). Neutrophil Integrin α9 Impairs Efferocytosis and Worsens Long-term Recovery after Subarachnoid Hemorrhage Arteriosclerosis, thrombosis, and vascular biology, (), 10.1161/ATVBAHA.125.323072. . PMCID: 12333561.


2.) Lee M, Wood BJ, Jeong HH, Nam HW, Keller CM, Lee B, Kim JI, Murnane KS, Goeders NE, Harris NR (2025 Jul 2). Retinal Angiogenesis in Methamphetamine Self-Administration Rats Investigative Ophthalmology & Visual Science, 66(9), 8. . PMCID: 12227027.


3.) Norris MJ, Henderson LA, Siddiquey MN, Yin J, Yoo K, Brunel S, Mor M, Saphire EO, Benedict CA, Kamil JP (2025 Jun 30). The GATE glycoprotein complex enhances human cytomegalovirus entry in endothelial cells Nature microbiology, 10(7), 1605-1616. . PMCID: 12312674.


4.) Keiffer TR, DiGiuseppe S, Guion L, Bienkowska-Haba M, Zwolinska K, Siddiqa A, Kushwaha A, Sapp MJ (2024 Dec 4). HPV16 entry requires dynein for minus-end transport and utilizes kinesin Kif11 for plus-end transport along microtubules during mitosis Journal of Virology, 99(1), e00937-24. . PMCID: 11784366.


5.) Norris MJ, Henderson LA, Siddiquey MN, Yin J, Yoo K, Brunel S, Saphire EO, Benedict CA, Kamil JP (2024 Oct 13). A noncanonical glycoprotein H complex enhances cytomegalovirus entry bioRxiv, (), 2024.10.13.617647. . PMCID: 11482907.


6.) Lee M, Leskova W, Eshaq RS, Amezquita Z, Harris NR (2024 Oct). Mechanisms of retinal photoreceptor loss in spontaneously hypertensive rats. Experimental eye research, 247(), 110065. . PMID: 39222765.


7.) Aishwarya R, Abdullah CS, Remex NS, Bhuiyan MA, Lu XH, Dhanesha N, Stokes KY, Orr AW, Kevil CG, Bhuiyan MS (2024 Jul 19). Diastolic dysfunction in Alzheimer’s disease model mice is associated with Aβ-amyloid aggregate formation and mitochondrial dysfunction Scientific Reports, 14(), 16715. . PMCID: 11271646.


8.) Pandey N, Kaur H, Chorawala MR, Anand SK, Chandaluri L, Butler ME, Aishwarya R, Gaddam SJ, Shen X, Alfaidi M, Wang J, Zhang X, Beedupalli K, Bhuiyan MS, Bhuiyan MA, Buchhanolla P, Rai P, Shah R, Chokhawala H, Jordan JD, Magdy T, Orr AW, Stokes KY, Rom O, Dhanesha N (2024 Mar 27). Interactions between integrin α9β1 and VCAM-1 promote neutrophil hyperactivation and mediate poststroke DVT Blood Advances, 8(9), 2104-2117. . PMCID: 11063402.


9.) Alvarez IA, Lee M, Eshaq RS, Leskova W, Harris NR (2024 Feb 6). High Glucose Induces Oxidative Stress That Alters Glycocalyx Proteoglycan Levels in Primary Rat Retinal Microvascular Endothelial Cells and in Isolated Ophthalmic Arteries. Pathophysiology : the official journal of the International Society for Pathophysiology, 31(1), 89-99. . PMID: 38390944.


Description:

The Research Core Facility (RCF) at Louisiana State University Health Sciences Center at Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational and training opportunities, and state-of-the-art technologies. Each technology is staffed by a trained research specialist.  These technologies include cellular metabolism, flow cytometry, high-performance computing, mass spectrometry, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, real-time PCR, and transmission electromagnetic microscopy. The Research Core Facility provides assistance with all aspects of these technologies, including experimental design, protocol optimization, instrument operation, and data analysis. All services are available on a fee-for-service basis to internal and external researchers.