University of Calgary
Contact Info:
Pina Colarusso
3330 Hospital Dr NW
HSC 2822
Calgary, AB T2N 4N1
https://snyder.ucalgary.ca/labs/live-cell-imaging/LCIoverview
Grants and Identifiers:
RRID: RRID:SCR_024748
Instrumentation:
Essen Incucyte Incucyte
IncuCyte ZOOM system is a live-cell imaging and analysis platform that enables automated quantification of cell behavior over time (from hours to weeks) by automatically gathering and analyzing images around the clock. The system provides insight into active biological processes in real-time which is not possible using single-point and end-point measurements. The system resides within the controlled environment of a standard cell incubator. All imaging is completely non-invasive and non-perturbing to cell health. The system can process multiple plates, flasks and dishes in parallel and does not depend on shuttling plates into and out of the incubator. [Product Link]
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Nikon A1R Confocal Laser Scanning Microscope
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
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Services Provided:
Confocal/ Super Resolution Microscope
Fluorescence Microscope With Optical Sectioning Capability
Multiphoton/ Harmonic Generation Microscope
Spinning Disk Confocal Imaging
Relevant Publications:
Description:
The LCI is community-driven shared technology platform. We leverage imaging and rapid prototyping to drive research and to translate new knowledge for societal benefit. We provide researchers to advanced imaging technology, as well as the training and support personalized to their imaging goals and experience. The LCI\'s interdisciplinary science and engineering team can help you achieve your specific imaging, design and prototyping needs. Flexible options include: -Technology access and training informed by best practices in education research -Assisted imaging and coaching at every step of the imaging workflow, from sample preparation to data analysis -Pilot experiments and feasibility testing -Developing custom image analysis pipelines Image analysis consultation and training -Developing and testing new instruments and devices -Full scientific and technological collaborations and partnerships -Offsite coaching, assistance and consulting available (local, national, international) -Collaborative projects in the academic, health and allied industrial sectors -External partnerships and contracts welcome The LCI\'s unique capabilities. expertise and innovative technology training have sparked partnerships and collaborations with global reach in the academic, health, and industrial sectors.
