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Flow Cytometry Core Facility

University of Nebraska–Lincoln

Contact Info:

Dirk Anderson

4240 Fair St

Morrison Center 163

Lincoln, NE 68583

https://biotech.unl.edu/flow-cytometry-0

Grants and Identifiers:

RRID: RRID:SCR_024711

Instrumentation:

(Equipment No Longer Active At this Facility)

Beckman Coulter CytoFLEX Flow Cytometer

Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]

RRID:SCR_019627

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Beckman Coulter CytoFLEX LX

Analyzer designed to measure FSC and SSC, as well as up to 16 fluorescence parameters.

Citation IDs: RRID:SCR_02067

cite this instrument

Beckman Coulter CytoFLEX SRT

Cell sorter that can sort up to four different populations from a sample.

Citation IDs: RRID SCR_025068

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NanoFCM NanoAnaylzer

Nanoparticle analyzer capable of measuring size and concentration of samples, as well as up to two fluorescent parameters

Citation IDs: RRID SCR_025069

cite this instrument

Services Provided:

Cell Sorting

Consultations

Exosomes Characterisation

Experimental Design

FACS Cell Sorting

Flow Cytometric Analysis

Flow Cytometry

Flow Cytometry Data Analysis

Multi-color Flow Cytometry

Sample Preparation

Single Particle Extracellular Vesicle Flow Cytometry

Staining

Relevant Publications:

Description:

The Flow Cytometry Core Facility provides University of Nebraska faculty, staff, students, and external academic and industry with high quality flow cytometry services. The facility currently has a Beckman Coulter CytoFLEX SRT sorter and CytoFLEX LX, as well as a NanoAnalyzer from NanoFCM. Facility staff are able to assist with everything from experimental design, instrument training, sample collection, and data analysis.