Tulane University

Contact Info:

Nicholas Maness, PhD

TNPRC Virus Characterization, Isolation, Production and Sequencing Core

18703 Three Rivers Road

Covington, LA 70433

https://tnprc.tulane.edu/virus-characterization-isolation-and-production-core

Grants and Identifiers:

RRID: RRID:SCR_024679

Instrumentation:

Services Provided:

Relevant Publications:

1.) Currey J, Ellsworth C, Khatun MS, Wang C, Chen Z, Liu S, Midkiff C, Xiao M, Ren M, Liu F, Elgazzaz M, Fox S, Maness NJ, Rappaport J, Lazartigues E, Blair R, Kolls JK, Mauvais-Jarvis F, Qin X (2024 Oct). Upregulation of inflammatory genes and pathways links obesity to severe COVID-19. Biochimica et biophysica acta. Molecular basis of disease, 1870(7), 167322. . PMID: 38942338.

2.) Wang C, Khatun MS, Ellsworth CR, Chen Z, Islamuddin M, Nisperuza Vidal AK, Afaque Alam M, Liu S, Mccombs JE, Maness NJ, Blair RV, Kolls JK, Qin X (2024 Sep 17). Deficiency of Tlr7 and Irf7 in mice increases the severity of COVID-19 through the reduced interferon production. Communications biology, 7(1), 1162. . PMID: 39289468.

3.) Ellsworth CR, Chen Z, Xiao MT, Qian C, Wang C, Khatun MS, Liu S, Islamuddin M, Maness NJ, Halperin JA, Blair RV, Kolls JK, Tomlinson S, Qin X (2024 Sep 16). Enhanced complement activation and MAC formation accelerates severe COVID-19 Cellular and Molecular Life Sciences: CMLS, 81(1), 405. . PMCID: 11405604.

4.) MacLean A, Horn M, Midkiff C, Van Zandt A, Saied A (2024 Aug 5). Combination antiretroviral therapy prevents SIV- induced aging in the hippocampus and neurodegeneration throughout the brain Research Square, (), rs.3.rs-4681317. . PMCID: 11326353.

5.) Ellsworth CR, Wang C, Katz AR, Chen Z, Islamuddin M, Yang H, Scheuermann SE, Goff KA, Maness NJ, Blair RV, Kolls JK, Qin X (2024 Apr 15). Natural Killer Cells Do Not Attenuate a Mouse-Adapted SARS-CoV-2-Induced Disease in Rag2−/− Mice Viruses, 16(4), 611. . PMCID: 11054502.

6.) Beddingfield BJ, Plante KS, Plante JA, Weaver SC, Bose S, Krzykwa C, Chirichella N, Redmann RK, Seiler SZ, Dufour J, Blair RV, Endt K, Volkmann A, Maness NJ, Roy CJ (2024 Feb 27). MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques NPJ Vaccines, 9(), 47. . PMCID: 10899228.

7.) Melton A, Rowe LA, Penney T, Krzykwa C, Goff K, Scheuermann S, Melton HJ, Williams K, Golden N, Green KM, Smith B, Russell-Lodrigue K, Dufour JP, Doyle-Meyers LA, Schiro F, Aye PP, Lifson JD, Beddingfield BJ, Blair RV, Bohm RP, Kolls JK, Rappaport J, Hoxie JA, Maness NJ (2023 Nov 16). The Impact of SIV-Induced Immunodeficiency on Clinical Manifestation, Immune Response, and Viral Dynamics in SARS-CoV-2 Coinfection. bioRxiv : the preprint server for biology, (), . . PMID: 38014096.

Description:

The Virus Characterization, Isolation, Production and Sequencing Core (VCIPS) core is divided into two primary components: 1) virus characterization, isolation, and production and 2) next generation sequencing. The Virus Characterization, Isolation and Production component provides virus expansion and characterization, viral titer by plaque and TCID50 assays, live virus neutralization and inhibition assays at both BSL-2 and -3 (including SARS-CoV-2). The Next Generation Sequencing component provides expertise in next generation sequencing and timely genomic services including whole genome, epigenetics, targeted amplicons, and 16S metagenomics.