UConn Health
Contact Info:
Yi Wu
263 Farmington Avenue
Farmington, CT 06030-6406
https://health.uconn.edu/cell-analysis-modeling/microscopy-facility/
Grants and Identifiers:
RRID: RRID:SCR_024655
Instrumentation:
Zeiss Axio Vert series Axiovert 200 inverted microscope
Inverted microscope that is used for the examination of cell and tissue culture of sediments in culture flasks, Petri dishes, microtiter plates, etc. in transmitted and reflected light. It hass attachment possibilities for various tools (micromanipulation), different light sources, temperature control devices. [Product Link]
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Zeiss Lightsheet Z.1 Lightsheet Fluorescence Microscope
Light sheet fluorescence microscope for the imaging of large cleared specimens. Lightsheet Z.1 with Clr Plan-Apochromat 20x/1.0 Corr nd=1.38 is used to perform experiments with tissue cleared by Scale medium (Hama et al, Nat Neurosci. 2011), which has a refractive index of n=1.38. [Product Link]
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Zeiss LSM 780 Confocal Laser Scanning Microscope
Laser Scanning Microscopes (LSM) for high resolution imaging of biological or material samples. The compact scanning module can be fitted to an inverted (Axio Observer.Z1 RP or SP) or upright (Axio Imager.Z2, Axio Imager.M2 or Axio Examiner) microscope. [Product Link]
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Nikon CREST V3 Spinning Disk Confocal
Nikon Eclipse Ti2 with Teledyne Photometrics Kinetix 10 MP sCMOS camera.
Services Provided:
Relevant Publications:
Description:
The CCAM Microscopy Facility provides the UConn Health research community as well as other academic and industrial institutes, access to its state-of-the-art equipment for quantitative fluorescence imaging applications. Within the facility, there are two laser scanning confocal microscopes equipped with 34-channel high-efficiency QUASAR detectors. One of these microscopes supports nonlinear optical (also known as 2-photon) excitation, fluorescence correlation spectroscopy (FCS), and fluorescence lifetime imaging microscopy (FLIM). Additionally, there is a light sheet microscope available for micro/mesoscale 3D imaging. The Zeiss Elyra 7 microscope is capable of total internal reflection fluorescence microscopy (TIRFM) and supports several super-resolution techniques such as lattice structured illumination microscopy (SIM), stochastic optical reconstruction microscopy (STORM), 3D photoactivated localization microscopy (PALM), and DNA points accumulation for imaging in nanoscale topography (DNA-PAINT). For live cell imaging we offer the Nikon Crest V3 Spinning Disk Confocal microscope with excitation wavelengths ranging from 405 nm - 754 nm. This system is also equipped with a stage top incubation system with CO2 and O2. CCAM also has two widefield systems fully equipped to handle live, long term cell imaging.
