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LIMicro-IQ - Microscopy Core Facility

Universidade Estadual de Campinas (UNICAMP)

Contact Info:

Hugo Loureiro/Microsocpy laboratory team

University of Campinas, Institute of Chemistry, Microscopy Laboratory - D106

Campinas, São Paulo

https://www.iqm.unicamp.br/en/research/limicro/

Grants and Identifiers:

RRID: RRID:SCR_024633

Instrumentation:

(Equipment No Longer Active At this Facility)

Leica TCS SP5 Broadband Confocal Laser Scanning Microscope

Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]

RRID:SCR_020233

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(Equipment No Longer Active At this Facility)

Nikon E800 E800

Building upon the success of the popular CFI60 optical system (60-millimeter parfocal distance and 25-millimeter objective thread size) first installed on the Eclipse E600, Nikon has moved a step further by introducing ultra-low magnification objectives and has increased the standard field of view to 25 millimeters. Introduced with the Eclipse E800 was a plan 0.5x magnification objective, which accommodates a 50-millimeter field of view at the unprecedented magnification of 0.5x. When coupled with a 2x projection lens, the 0.5x objective permits macrophotography at 1:1 magnification (in effect, actual size documentation). The new macro objective lens is particularly useful for investigating large specimens such as brain slices, developing embryos, and extensive tissue sections. This objective is easily accommodated into the microscope without major re-configuration, and is interchangeable with other objectives on a standard rotating nosepiece. [Product Link]

RRID:SCR_020326

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(Equipment No Longer Active At this Facility)

Nikon Eclipse 800 Widefield Microscope

Eclipse 800 Microscope is a premier member of Nikon's advanced research microscope line. The E800 series incorporates Nikon's CFI60 optical system that (according to them) alleviates the limitations of conventional optics, allowing for very long working distances (for a tutorial on Nikon's CFI60 Optical System, click here). This system corrects chromatic aberrations over the entire field of view, generating a very sharp, clear image over the broadest magnification range. One can also put in a broad range of accessories without affecting optical quality. The microscope is designed so that the scientist can work with ease, especially in the dark, requiring little movement to operate the control knobs and handles. The CFI60 Optical System offers a 60mm objective parfocal distance, a 25mm objective thread size and a standard 25mm field of view all of which combine to provide a higher optical quality. [Product Link]

RRID:SCR_020323

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FEI Quanta FEG 250

Field Emission Scanning Electron Microscope

Citation IDs: Field Emission Gun Scanning Electron Microscope

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(Equipment No Longer Active At this Facility)

Nanosurf Flex AFM with C3000 controller

Atomic Force Microscope

Citation IDs: FAPESP n. 2017/ 11986-5

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Nanosurf FlexAFM with C3000 controller

Citation IDs: FAPESP (2017/ 11986-5)

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PicoQuant MicroTime 200

Confocal Laser Scanning Microscope with single molecule detection and Time Correlated Single Photon Counting (TCSPC)

Citation IDs: FAPESP (2023/16510-0)

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Services Provided:

Confocal Microscopy

Cryo-Ultra Microtomy

Data Analysis And Interpretation

Electron Microscopy

Fluorescence Microscope With Optical Sectioning Capability

Kelvin Probe Force Microscopy

Microscopy

Scanning Electron Microscopy

Relevant Publications:

1.) Messias I, Winkler MEG, Costa GF, Mariano T, Souza Junior JB, Neckel IT, Figueiredo MC, Singh N, Nagao R (2024 Oct 14). Role of Structural and Compositional Changes of Cu(2)O Nanocubes in Nitrate Electroreduction to Ammonia. ACS applied energy materials, 7(19), 9034-9044. . PMID: 39421273.


2.) Tiroli-Cepeda AO, Linhares LA, Aragão AZB, de Jesus JR, Wasilewska-Sampaio AP, De Felice FG, Ferreira ST, Borges JC, Cyr DM, Ramos CHI (2024 Sep). Type I Hsp40s/DnaJs aggregates exhibit features reminiscent of amyloidogenic structures. The FEBS journal, 291(17), 3904-3923. . PMID: 38975859.


3.) de Lima LF, Barbosa PP, Simeoni CL, de Paula RFO, Proenca-Modena JL, de Araujo WR (2023 Dec 20). Electrochemical Paper-Based Nanobiosensor for Rapid and Sensitive Detection of Monkeypox Virus. ACS applied materials & interfaces, 15(50), 58079-58091. doi: 10.1021/acsami.3c10730. PMID: 38063784.


Description:

The Microscopy Laboratory at the Institute of Chemistry, Unicamp, is committed to advancing scientific research in the field of chemistry. Our mission is to offer comprehensive support to researchers and corporate users. Our well-equipped laboratory includes essential instruments like Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Confocal Laser Scanning Microscopy (CLSM), and Optical Microscopy. Additionally, we provide sample preparation techniques, including ultramicrotome, ultracryomicrotome, and sputter coating. We also offer training and technical support.