Tulane University
Nadia Golden
TNPRC High Containment Research Performance Core
18703 Three Rivers Road
Covington, LA 70433
https://tnprc.tulane.edu/hcrpc
RRID: RRID:SCR_024612
Life Technologies QuantStudio 6 Real Time PCR System
QuantStudio 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well block in this case); other block types may be purchased separately. For a system that also supports TaqMan array cards, as well as automation, please see our QuantStudio 7 Flex system. The QuantStudio 6 Flex system can also be upgraded to a QuantStudio 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio 12K Flex system, which also accommodates TaqMan OpenArray plates. [Product Link]
RRID:SCR_020239
Miltenyi gentleMACS Octo Dissociator Tissue Dissociator
Benchtop instrument for semi automated and standardized tissue dissociation or homogenization of up to eight samples. Single cell suspensions or thorough homogenates from virtually any tissues are reproducibly obtained using uniquecanTubes or M Tubes. [Product Link]
RRID:SCR_020272
Biorad Bio-plex 200 system
Suspension array system which offers protein and nucleic acid multiplex assays
MSD Mesoscale QuickPlex SQ120 Imager
Multiplexing plate reading. Electrochemiluminescence Immunoassay for detecting biomarkers and proteins in biological samples.
O'Link O'Link Signature Q100 System
Developed specifically for O'link target and focus protein biomarker panels.
Zeiss Axio Oberver.7 Microscope
open and flexible inverted microscope platform for multimodal imaging of living and fixed specimens.
Assay Development
Assays and Measurements
Blood Cell Count
Blood Processing
CDNA Synthesis
Cytokine Assays
Data Analysis
ELISA
Flow Cytometry
Immunization
Immunoassay
Live Cell Imaging
Luminex Bead Based Cytokine Assays
Necropsy
Nucleic Acid Extraction
Protocol Development
Real-time qPCR
RNA Isolation
Sample Preparation
Tissue Embed
Tissue Extraction
1.) Fredericks MN, Kolodner Z, Waalkes A, Sawatzki K, Hao L, Long DR, Penewit K, Midkiff CC, McCormick CJ, Beraki S, Edlefsen PT, Barrow J, Greninger AL, Gale M, Blair RV, Salipante SJ, Fuller DH, O’Connor MA (2025 Mar 26). SIV/SARS-CoV-2 co-infection in rhesus macaques impacts viral shedding, host immunity, the microbiome, and viral evolution Research Square, (), rs.3.rs-6033850. . PMCID: 11975012.
2.) Wang C, Khatun MS, Ellsworth CR, Chen Z, Islamuddin M, Nisperuza Vidal AK, Afaque Alam M, Liu S, Mccombs JE, Maness NJ, Blair RV, Kolls JK, Qin X (2024 Sep 17). Deficiency of Tlr7 and Irf7 in mice increases the severity of COVID-19 through the reduced interferon production. Communications biology, 7(1), 1162. . PMID: 39289468.
3.) Ellsworth CR, Chen Z, Xiao MT, Qian C, Wang C, Khatun MS, Liu S, Islamuddin M, Maness NJ, Halperin JA, Blair RV, Kolls JK, Tomlinson S, Qin X (2024 Sep 16). Enhanced complement activation and MAC formation accelerates severe COVID-19 Cellular and Molecular Life Sciences: CMLS, 81(1), 405. . PMCID: 11405604.
4.) Edwards CT, Karunakaran KA, Garcia E, Beutler N, Gagne M, Golden N, Aoued H, Pellegrini KL, Burnett MR, Honeycutt CC, Lapp SA, Ton T, Lin MC, Metz A, Bombin A, Goff K, Scheuermann SE, Wilkes A, Wood JS, Ehnert S, Weissman S, Curran EH, Roy M, Dessasau E, Paiardini M, Upadhyay AA, Moore I, Maness NJ, Douek DC, Piantadosi A, Andrabi R, Rogers TR, Burton DR, Bosinger SE (2024 Jul 30). Passive infusion of an S2-Stem broadly neutralizing antibody protects against SARS-CoV-2 infection and lower airway inflammation in rhesus macaques. bioRxiv : the preprint server for biology, (), . . PMID: 39109178.
5.) Currey J, Ellsworth C, Khatun MS, Wang C, Chen Z, Liu S, Midkiff C, Xiao M, Ren M, Liu F, Elgazzaz M, Fox S, Maness NJ, Rappaport J, Lazartigues E, Blair R, Kolls JK, Mauvais-Jarvis F, Qin X (2024 Jun 26). Upregulation of inflammatory genes and pathways links obesity to severe COVID-19 Biochimica et biophysica acta. Molecular basis of disease, 1870(7), 167322. . PMCID: 11330358.
6.) Ellsworth CR, Wang C, Katz AR, Chen Z, Islamuddin M, Yang H, Scheuermann SE, Goff KA, Maness NJ, Blair RV, Kolls JK, Qin X (2024 Apr 15). Natural Killer Cells Do Not Attenuate a Mouse-Adapted SARS-CoV-2-Induced Disease in Rag2(-/-) Mice. Viruses, 16(4), . . PMID: 38675952.
7.) Melton A, Rowe LA, Penney T, Krzykwa C, Goff K, Scheuermann S, Melton HJ, Williams K, Golden N, Green KM, Smith B, Russell-Lodrigue K, Dufour JP, Doyle-Meyers LA, Schiro F, Aye PP, Lifson JD, Beddingfield BJ, Blair RV, Bohm RP, Kolls JK, Rappaport J, Hoxie JA, Maness NJ (2023 Nov 16). The Impact of SIV-Induced Immunodeficiency on Clinical Manifestation, Immune Response, and Viral Dynamics in SARS-CoV-2 Coinfection. bioRxiv : the preprint server for biology, (), . . PMID: 38014096.
8.) Routhu NK, Stampfer SD, Lai L, Akhtar A, Tong X, Yuan D, Chicz TM, McNamara RP, Jakkala K, Davis-Gardner ME, St Pierre EL, Smith B, Green KM, Golden N, Picou B, Jean SM, Wood J, Cohen J, Moore IN, Patel N, Guebre-Xabier M, Smith G, Glenn G, Kozlowski PA, Alter G, Ahmed R, Suthar MS, Amara RR (2023 Oct 27). Efficacy of mRNA-1273 and Novavax ancestral or BA.1 spike booster vaccines against SARS-CoV-2 BA.5 infection in nonhuman primates. Science immunology, 8(88), eadg7015. . ID: 37191508.
9.) Fears AC, Walker EM, Chirichella N, Slisarenko N, Merino KM, Golden N, Picou B, Spencer S, Russell-Lodrigue KE, Doyle-Meyers LA, Blair RV, Beddingfield BJ, Maness NJ, Roy CJ, Rout N (2022 Dec 16). The dynamics of γδ T cell responses in nonhuman primates during SARS-CoV-2 infection. Communications biology, 5(1), 1380. . ID: 36526890.
10.) Beddingfield BJ, Rowe LA, Russell-Lodrigue KE, Doyle-Meyers LA, Golden N, Spencer S, Chirichella N, Blair RV, Maness NJ, Roy CJ (2022 Nov 1). Breakthrough Gastrointestinal COVID-19 and Intrahost Evolution Consequent to Combination Monoclonal Antibody Prophylaxis. The Journal of infectious diseases, 226(9), 1588-1592. . ID: 35429402.
11.) Arunachalam PS, Feng Y, Ashraf U, Hu M, Walls AC, Edara VV, Zarnitsyna VI, Aye PP, Golden N, Miranda MC, Green KWM, Threeton BM, Maness NJ, Beddingfield BJ, Bohm RP, Scheuermann SE, Goff K, Dufour J, Russell-Lodrigue K, Kepl E, Fiala B, Wrenn S, Ravichandran R, Ellis D, Carter L, Rogers K, Shirreff LM, Ferrell DE, Deb Adhikary NR, Fontenot J, Hammond HL, Frieman M, Grifoni A, Sette A, O (2022 Aug 17). Durable protection against the SARS-CoV-2 Omicron variant is induced by an adjuvanted subunit vaccine. Science translational medicine, 14(658), eabq4130. . ID: 35976993.
12.) Fears AC, Beddingfield BJ, Chirichella NR, Slisarenko N, Killeen SZ, Redmann RK, Goff K, Spencer S, Picou B, Golden N, Midkiff CC, Bush DJ, Branco LM, Boisen ML, Gao H, Montefiori DC, Blair RV, Doyle-Meyers LA, Russell-Lodrigue K, Maness NJ, Roy CJ (2022 Jul). Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate species. PLoS pathogens, 18(7), e1010618. . ID: 35789343.
13.) Rutkai I, Mayer MG, Hellmers LM, Ning B, Huang Z, Monjure CJ, Coyne C, Silvestri R, Golden N, Hensley K, Chandler K, Lehmicke G, Bix GJ, Maness NJ, Russell-Lodrigue K, Hu TY, Roy CJ, Blair RV, Bohm R, Doyle-Meyers LA, Rappaport J, Fischer T (2022 Apr 1). Neuropathology and virus in brain of SARS-CoV-2 infected non-human primates. Nature communications, 13(1), 1745. . ID: 35365631.
14.) Pillet S, Arunachalam PS, Andreani G, Golden N, Fontenot J, Aye PP, Röltgen K, Lehmicke G, Gobeil P, Dubé C, Trépanier S, Charland N, D (2022 Feb). Safety, immunogenicity, and protection provided by unadjuvanted and adjuvanted formulations of a recombinant plant-derived virus-like particle vaccine candidate for COVID-19 in nonhuman primates. Cellular & molecular immunology, 19(2), 222-233. . ID: 34983950.
15.) Redmann RK, Kaushal D, Golden N, Threeton B, Killeen SZ, Kuehl PJ, Roy CJ (2022 Jan). Particle Dynamics and Bioaerosol Viability of Aerosolized Bacillus Calmette-Guérin Vaccine Using Jet and Vibrating Mesh Clinical Nebulizers. Journal of aerosol medicine and pulmonary drug delivery, 35(1), 50-56. . ID: 34619040.
16.) Melton A, Doyle-Meyers LA, Blair RV, Midkiff C, Melton HJ, Russell-Lodrigue K, Aye PP, Schiro F, Fahlberg M, Szeltner D, Spencer S, Beddingfield BJ, Goff K, Golden N, Penney T, Picou B, Hensley K, Chandler KE, Plante JA, Plante KS, Weaver SC, Roy CJ, Hoxie JA, Gao H, Montefiori DC, Mankowski JL, Bohm RP, Rappaport J, Maness NJ (2021 Dec). The pigtail macaque (Macaca nemestrina) model of COVID-19 reproduces diverse clinical outcomes and reveals new and complex signatures of disease. PLoS pathogens, 17(12), e1010162. . ID: 34929014.
17.) Arunachalam PS, Walls AC, Golden N, Atyeo C, Fischinger S, Li C, Aye P, Navarro MJ, Lai L, Edara VV, Röltgen K, Rogers K, Shirreff L, Ferrell DE, Wrenn S, Pettie D, Kraft JC, Miranda MC, Kepl E, Sydeman C, Brunette N, Murphy M, Fiala B, Carter L, White AG, Trisal M, Hsieh CL, Russell-Lodrigue K, Monjure C, Dufour J, Spencer S, Doyle-Meyers L, Bohm RP, Maness NJ, Roy C, Plante JA, Plante KS, Zhu A, Gorman MJ, Shin S, Shen X, Fontenot J, Gupta S, O (2021 Jun). Adjuvanting a subunit COVID-19 vaccine to induce protective immunity. Nature, 594(7862), 253-258. doi: 10.1038/s41586-021-03530-2. ID: 33873199.
18.) S Arunachalam P, Walls AC, Golden N, Atyeo C, Fischinger S, Li C, Aye P, Navarro MJ, Lai L, Edara VV, Roltgen K, Rogers K, Shirreff L, Ferrell DE, Wrenn S, Pettie D, Kraft JC, Miranda MC, Kepl E, Sydeman C, Brunette N, Murphy M, Fiala B, Carter L, White AG, Trisal M, Hsieh CL, Russell-Lodrigue K, Monjure C, Dufour J, Doyle-Meyer L, Bohm RB, Maness NJ, Roy C, Plante JA, Plante KS, Zhu A, Gorman MJ, Shin S, Shen X, Fontenot J, Gupta S, O Hagan DT, Most RV, Rappuoli R, Coffman RL, Novack D, McLella (2021 Feb 11). Adjuvanting a subunit SARS-CoV-2 nanoparticle vaccine to induce protective immunity in non-human primates. bioRxiv : the preprint server for biology, (), . . ID: 33594366.
19.) Blair RV, Vaccari M, Doyle-Meyers LA, Roy CJ, Russell-Lodrigue K, Fahlberg M, Monjure CJ, Beddingfield B, Plante KS, Plante JA, Weaver SC, Qin X, Midkiff CC, Lehmicke G, Golden N, Threeton B, Penney T, Allers C, Barnes MB, Pattison M, Datta PK, Maness NJ, Birnbaum A, Fischer T, Bohm RP, Rappaport J (2021 Feb). Acute Respiratory Distress in Aged, SARS-CoV-2-Infected African Green Monkeys but Not Rhesus Macaques. The American journal of pathology, 191(2), 274-282. . ID: 33171111.
20.) Han K, Blair RV, Iwanaga N, Liu F, Russell-Lodrigue KE, Qin Z, Midkiff CC, Golden NA, Doyle-Meyers LA, Kabir ME, Chandler KE, Cutrera KL, Ren M, Monjure CJ, Lehmicke G, Fischer T, Beddingfield B, Wanek AG, Birnbaum A, Maness NJ, Roy CJ, Datta PK, Rappaport J, Kolls JK, Qin X (2021 Jan). Lung Expression of Human Angiotensin-Converting Enzyme 2 Sensitizes the Mouse to SARS-CoV-2 Infection. American journal of respiratory cell and molecular biology, 64(1), 79-88. . ID: 32991819.
21.) Ning B, Yu T, Zhang S, Huang Z, Tian D, Lin Z, Niu A, Golden N, Hensley K, Threeton B, Lyon CJ, Yin XM, Roy CJ, Saba NS, Rappaport J, Wei Q, Hu TY (2021 Jan). A smartphone-read ultrasensitive and quantitative saliva test for COVID-19. Science advances, 7(2), . . ID: 33310733.
22.) Coleman C, Doyle-Meyers LA, Russell-Lodrigue KE, Golden N, Threeton B, Song K, Pierre G, Baribault C, Bohm RP, Maness NJ, Kolls JK, Rappaport J, Mudd JC (2021). Similarities and Differences in the Acute-Phase Response to SARS-CoV-2 in Rhesus Macaques and African Green Monkeys. Frontiers in immunology, 12(), 754642. . ID: 34691074.
The High Containment Research Performance Core (HCRPC) provides full or partial support for researchers seeking to perform BSL3 and BSL2/BSL3 Select Agent studies and provides services for bacterial/viral culture and propagation, sample preparation, and processing of blood, BAL, single cell isolation, flow cytometry, and more. This core offers project management assistance in study design, budgeting, and IACUC and IBC preparation. Our standard operating procedures have been tested to ensure consistent practices and have undergone rigorous quality assurance processes to ensure reproducibility. The HCRPC offers assay services for qPCR, O’link Q100, Biorad Bioplex 200 , Meso QuickPlex SQ120 Imager and the Microscope Axio Observer.
