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TNPRC Flow Cytometry

Tulane University

Contact Info:

Amitinder Kaur, MD

TNPRC Flow Cytometry

18703 Three Rivers Road

Covington, LA 70433

https://tnprc.tulane.edu/flow-cytometry

Grants and Identifiers:

RRID: RRID:SCR_024611

Instrumentation:

BD LSRFortessa Fortessa Flow Cytometer

BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]

RRID:SCR_019601

Citation IDs: Center for AIDS Research award number 5P30AI050410

cite this instrument

Miltenyi Bioscience MACSQuant Analyzer 16

Automated flow cytometer with expanded fluorescence capabilities, enabling measurement of up to 16 parameters. [Product Link]

RRID:SCR_020263

cite this instrument

BD Biosciences BD FACSAria Fusion SORP

Flow Cytometry Sorter capable of 18-color detection and high-speed four-way sorting

BD Biosciences BD FACSMelody

Cell Sorter

BD Biosciences BD FACSymphony A5

30-parameter cell analyzer

BD Biosciences BD LSR II

Flow Cytometer Analyzer

Miltenyi Biotec MACSQuant Tyto

Cartridge cell sorter

Services Provided:

Assay Development

Blood Cell Count

Cell Sorting

Consultation

Consultations

Cytokine Assays

Data Analysis

FACS Cell Sorting

Flow Cytometric Analysis

Flow Cytometry

Flow Cytometry Data Analysis

High Dimensional Flow Cytometry Analysis

Immune Monitoring

Immunofluorescence

Multi-color Flow Cytometry

Single-Cell Analysis

Spectral Flow Cytometry

Staining

Training

Relevant Publications:

Description:

The Flow Cytometry Core provides analytical flow cytometry services and live cell sorting under enhanced Biosafety Level 2 (BSL2+) conditions. Instruments available include six analyzers ranging from 4-30 color acquisition and three sorters using 1-,2-,4-way, and plate sorting between 6-18 colors. We provide experimental and panel design consultation as a service. The core also provides continued education in seminar format and one-on-one sessions with user. The core is heavily utilized and supports research projects for a significant number of core and affiliate scientists.