Tulane University
Amitinder Kaur, MD
TNPRC Flow Cytometry
18703 Three Rivers Road
Covington, LA 70433
https://tnprc.tulane.edu/cores-and-services/flow-cytometry
RRID: RRID:SCR_024611
Other Citation Identifiers 5P51OD011104-59
1S10OD026800-01
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
RRID:SCR_019601
Miltenyi Bioscience MACSQuant Analyzer 16
Automated flow cytometer with expanded fluorescence capabilities, enabling measurement of up to 16 parameters. [Product Link]
RRID:SCR_020263
BD Biosciences BD FACSAria Fusion SORP
Flow Cytometry Sorter capable of 18-color detection and high-speed four-way sorting
BD Biosciences BD FACSMelody
Cell Sorter
BD Biosciences BD FACSymphony A5
30-parameter cell analyzer
BD Biosciences BD FACSymphony A5 SE
Spectral Symphony A5 with 5-lasers, 50 detectors, FFFS, and HTS
BD Biosciences BD LSR II
Flow Cytometer Analyzer
Miltenyi Biotec MACSQuant Tyto
Cartridge cell sorter
Assay Development
Blood Cell Count
Cell Sorting
Consultation
Consultations
Cytokine Assays
Data Analysis
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry
Flow Cytometry Data Analysis
High Dimensional Flow Cytometry Analysis
Immune Monitoring
Immunofluorescence
Multi-color Flow Cytometry
Single-Cell Analysis
Spectral Flow Cytometry
Staining
Training
1.) Nemphos SM, Green HC, Prusak JE, Fell SL, Midkiff C, Rodgers A, Perret J, Goff K, Miller J, Varnado M, Didier K, Valencia N, Moström MJ, Tatum C, Barnes MB, Krzykwa CE, Rowe LA, Allers C, Grasperge B, De Paris K, Maness NJ, Kaur A, Londono-Renteria B, Blair RV, Manuzak JA (2026). Gastrointestinal Mucosal Disruptions During ART-Treated SIV/Plasmodium fragile Co-Infection. Pathogens and Immunity, 11(1), 39-65. . PMCID: 12880234.
2.) Rhodehouse K, Zitzmann C, Ravi M, Armstrong C, Moskovljevic M, Moore HP, Schill C, Fray EJ, Kumar MR, Penney T, Krzykwa C, Stauss MR, Wiseman RW, O’Connor DH, Fennessey CM, Keele BF, Lifson JD, Ribeiro RM, Perelson AS, Hoxie JA, Maness NJ, Siliciano JD, Siliciano RF (2025 Dec 5). Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect The Journal of Experimental Medicine, 223(2), e20251172. . PMCID: 12679998.
3.) Van Zandt AR, Horn MD, McNamara RP, Peterson TA, Maness NJ, Schoest B, Frost EM, Zhou Y, Moström MJ, Dittmer DP, MacLean AG (2025 Oct 24). Simian Immunodeficiency Virus-Derived Extracellular Vesicles Induce a Chronic Inflammatory Phenotype in Healthy Astrocytes Unresolved by Anti-Retroviral Therapy. Pharmaceutics, 17(11), . . PMCID: 12655024.
4.) Van Zandt AR, Horn MD, Peterson TA, Dickinson SY, Frost EM, MacLean AG (2025 Sep 30). THC reverses SIV-induced senescence in astrocytes: possible compensatory mechanism against HIV associated brain injury? Frontiers in Cellular Neuroscience, 19(), 1642917. . PMCID: 12521829.
5.) Fell SL, Nemphos SM, Prusak JE, Green HC, Miller J, Rowan SQ, Valencia N, Tatum C, Barnes MB, Allers C, Scheuermann S, Goff K, Krzykwa C, Rowe LA, Maness NJ, Moström MJ, Hensley-McBain T, Doyle-Meyers L, Kaur A, Manuzak JA (2025 Sep 1). Inflammatory neutrophil responses and T cell activation in ART-treated SIVmac239-infected rhesus macaques. Journal of immunology (Baltimore, Md. : 1950), 214(9), 2307-2324. . PMID: 40504034.
6.) Melton A, Potter EL, Penney T, Krzykwa C, Rowe LA, Goff K, Scheuermann SE, Russell-Lodrigue K, Melton HJ, Magnani DM, Kolls JK, Blair RV, Maness NJ (2025 Jun 20). Distinct transcriptomic signatures in pulmonary tissue resident and vascular resident-like T cells in nonhuman primates. iScience, 28(6), 112745. . PMID: 40546965.
7.) Ma L, Nishimura Y, Wu X, Donau O, Vincent E, Lu H, Blair RV, Doyle-Meyers LA, Martin M, Veazey RS, Xu H, Wang X (2025 Jun 14). Impact of Delayed Early Antiretroviral Therapy Initiation on Treatment Outcomes in Infant Macaques Exposed to SHIVAD8. Viruses, 17(6), . . PMID: 40573440.
8.) Wu C, Johnson NM, Yu S, Lo AS, Sahu GK, Marx PA, von Laer D, Skowron G, Geleziunas R, Shaw GM, Kaur A, Junghans RP, Braun SE (2025 May 20). Persistence of CMV-specific anti-HIV CAR T cells after adoptive immunotherapy. Journal of virology, 99(5), e0193324. . PMID: 40207929.
9.) Reifel KM, Aspland A, Dervish S, Douagi I, Fears AC, Jellison ER, Midkiff CC, Mockus-Daehn T, Moström MJ, Solga M, Swan BK, Thomas J, Perfetto S (2025 Mar). Verifying Proper Function of the Aerosol Evacuation System Prior to Sorting Potentially Infectious Samples. Current protocols, 5(3), e70123. . PMID: 40116472.
10.) Thirugnanam S, Wang C, Zheng C, Grasperge BF, Datta PK, Rappaport J, Qin X, Rout N (2024 Aug 9). The Association between IL-1β and IL-18 Levels, Gut Barrier Disruption, and Monocyte Activation during Chronic Simian Immunodeficiency Virus Infection and Long-Term Suppressive Antiretroviral Therapy. International journal of molecular sciences, 25(16), . . PMCID: 11354606.
11.) Melton A, Rowe LA, Penney T, Krzykwa C, Goff K, Scheuermann SE, Melton HJ, Williams K, Golden N, Green KM, Smith B, Russell-Lodrigue K, Dufour JP, Doyle-Meyers LA, Schiro F, Aye PP, Lifson JD, Beddingfield BJ, Blair RV, Bohm RP, Kolls JK, Rappaport J, Hoxie JA, Maness NJ (2024 Jul 21). The Impact of SIV-Induced Immunodeficiency on SARS-CoV-2 Disease, Viral Dynamics, and Antiviral Immune Response in a Nonhuman Primate Model of Coinfection. Viruses, 16(7), . . PMID: 39066335.
12.) Nemphos SM, Green HC, Prusak JE, Fell SL, Goff K, Varnado M, Didier K, Guy N, Moström MJ, Tatum C, Massey C, Barnes MB, Rowe LA, Allers C, Blair RV, Embers ME, Maness NJ, Marx PA, Grasperge B, Kaur A, De Paris K, Shaffer JG, Hensley-McBain T, Londono-Renteria B, Manuzak JA (2024 Jun 27). Elevated Inflammation Associated with Markers of Neutrophil Function and Gastrointestinal Disruption in Pilot Study of Plasmodium fragile Co-Infection of ART-Treated SIVmac239+ Rhesus Macaques. Viruses, 16(7), . . PMID: 39066199.
13.) Thirugnanam S, Wang C, Zheng C, Grasperge BF, Datta PK, Rappaport J, Qin X, Rout N (2024 Jun 17). High IL-1β and IL-18 Levels Associate with Gut Barrier Disruption and Monocyte Activation During Chronic SIV Infection with Long-Term Suppressive Antiretroviral Therapy. bioRxiv : the preprint server for biology, (), . . PMID: 38948748.
14.) Zenere G, Wu C, Midkiff CC, Johnson NM, Grice CP, Wimley WC, Kaur A, Braun SE (2024). Extracellular domain, hinge, and transmembrane determinants affecting surface CD4 expression of a novel anti-HIV chimeric antigen receptor (CAR) construct. PloS one, 19(8), e0293990. . PMID: 39133676.
15.) Melton A, Rowe LA, Penney T, Krzykwa C, Goff K, Scheuermann S, Melton HJ, Williams K, Golden N, Green KM, Smith B, Russell-Lodrigue K, Dufour JP, Doyle-Meyers LA, Schiro F, Aye PP, Lifson JD, Beddingfield BJ, Blair RV, Bohm RP, Kolls JK, Rappaport J, Hoxie JA, Maness NJ (2023 Nov 16). The Impact of SIV-Induced Immunodeficiency on Clinical Manifestation, Immune Response, and Viral Dynamics in SARS-CoV-2 Coinfection. bioRxiv : the preprint server for biology, (), . . PMID: 38014096.
The Flow Cytometry Core provides analytical flow cytometry services and live cell sorting under enhanced Biosafety Level 2 (BSL2+) conditions.
Instruments available include six analyzers ranging from 4-30 color acquisition and three sorters using 1-,2-,4-way, and plate sorting between 6-18 colors. We provide experimental and panel design consultation as a service.
The core also provides continued education in seminar format and one-on-one sessions with user. The core is heavily utilized and supports research projects for a significant number of core and affiliate scientists.
The Flow Cytometry Core at the Tulane National Primate Research Center was awarded the ISAC Shared Resource Laboratory Recognition in April of 2025. This designation is given to flow cytometry facilities that adhere to internationally recognized best practices. This recognition is awarded between 2025-2028 with possibility of applying for continued recognition.
