Tulane University
Robert Blair, DVM, PhD
TNPRC Confocal Microscopy and Molecular Pathology Core
18703 Three Rivers Road
Covington, LA 70433
https://tnprc.tulane.edu/confocal-microscopy-and-molecular-pathology-core
RRID: RRID:SCR_024613
(Equipment No Longer Active At this Facility)
Leica TCS SP5 Broadband Confocal Laser Scanning Microscope
Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]
RRID:SCR_020233
ZEISS Axio Scan.Z1 Slide Scanner
Digitize your specimens with the Axio Scan.Z1 slide scanner and create high-quality virtual slides. Axio Scan.Z1 tackles the most demanding virtual microscopy research tasks as easily as it handles your routine work. The software module ZEN slidescan is designed specifically for the workflow of capturing virtual slides, while ZEN image analysis tools prepare your data accurately. Organize your virtual slides with ZEN browser, the web-based database, then view your data from any location using any operating system , or share virtual microscopy images online with colleagues and organize your projects, even when you are on the go. [Product Link]
RRID:SCR_020927
Leica TCS SP8 Confocal Microscope
Confocal Microscope
NanoString CosMX Spatial Molecular Imager
NanoString GeoMX Digital Spatial Profiler
Zeiss Observer 7 Fluorescent Microscope
Brightfield Microscopy
Confocal Microscopy
Consultation
Fluorescence Microscope With Optical Sectioning Capability
Image Aquisition
Image Processing And Analysis
Immunofluorescence
Immunohistochemistry
In Situ Hybridization
Microscopy
Staining
1.) Melton A, Rowe LA, Penney T, Krzykwa C, Goff K, Scheuermann S, Melton HJ, Williams K, Golden N, Green KM, Smith B, Russell-Lodrigue K, Dufour JP, Doyle-Meyers LA, Schiro F, Aye PP, Lifson JD, Beddingfield BJ, Blair RV, Bohm RP, Kolls JK, Rappaport J, Hoxie JA, Maness NJ (2023 Nov 16). The Impact of SIV-Induced Immunodeficiency on Clinical Manifestation, Immune Response, and Viral Dynamics in SARS-CoV-2 Coinfection. bioRxiv : the preprint server for biology, (), . . ID: 38014096.
2.) Moström MJ, Yu S, Tran D, Saccoccio FM, Versoza CJ, Malouli D, Mirza A, Valencia S, Gilbert M, Blair RV, Hansen S, Barry P, Früh K, Jensen JD, Pfeifer SP, Kowalik TF, Permar SR, Kaur A (2023 Oct). Protective effect of pre-existing natural immunity in a nonhuman primate reinfection model of congenital cytomegalovirus infection. PLoS pathogens, 19(10), e1011646. . ID: 37796819.
3.) Gonzalez AA, Olsen EL, Killeen SZ, Blair RV, Seshan SV, Jaimes EA, Roy CJ, Prieto MC (2023 Apr). Elevated soluble urokinase plasminogen activator receptor is associated with renal dysfunction in a Chlorocebus atheiops COVID-19 model. Journal of medical primatology, 52(2), 131-134. . ID: 36377612.
4.) Maity S, Mayer MG, Shu Q, Linh H, Bao D, Blair RV, He Y, Lyon CJ, Hu TY, Fischer T, Fan J (2023 Apr). Cerebrospinal Fluid Protein Markers Indicate Neuro-Damage in SARS-CoV-2-Infected Nonhuman Primates. Molecular & cellular proteomics : MCP, 22(4), 100523. . ID: 36870567.
5.) Beddingfield BJ, Maness NJ, Spencer S, Rappaport J, Aye PP, Russell-Lodrigue K, Doyle-Meyers LA, Blair RV, Gao H, Montefiori D, Roy CJ (2023). Mid-titer human convalescent plasma administration results in suboptimal prophylaxis against SARS-CoV-2 infection in rhesus macaques. Frontiers in immunology, 14(), 1085883. . ID: 36845143.
6.) Fears AC, Walker EM, Chirichella N, Slisarenko N, Merino KM, Golden N, Picou B, Spencer S, Russell-Lodrigue KE, Doyle-Meyers LA, Blair RV, Beddingfield BJ, Maness NJ, Roy CJ, Rout N (2022 Dec 16). The dynamics of γδ T cell responses in nonhuman primates during SARS-CoV-2 infection. Communications biology, 5(1), 1380. . ID: 36526890.
7.) Beddingfield BJ, Rowe LA, Russell-Lodrigue KE, Doyle-Meyers LA, Golden N, Spencer S, Chirichella N, Blair RV, Maness NJ, Roy CJ (2022 Nov 1). Breakthrough Gastrointestinal COVID-19 and Intrahost Evolution Consequent to Combination Monoclonal Antibody Prophylaxis. The Journal of infectious diseases, 226(9), 1588-1592. . ID: 35429402.
8.) Fears AC, Beddingfield BJ, Chirichella NR, Slisarenko N, Killeen SZ, Redmann RK, Goff K, Spencer S, Picou B, Golden N, Midkiff CC, Bush DJ, Branco LM, Boisen ML, Gao H, Montefiori DC, Blair RV, Doyle-Meyers LA, Russell-Lodrigue K, Maness NJ, Roy CJ (2022 Jul). Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate species. PLoS pathogens, 18(7), e1010618. . ID: 35789343.
9.) Lawrence SP, Elser SE, Torben W, Blair RV, Pahar B, Aye PP, Schiro F, Szeltner D, Doyle-Meyers LA, Haggarty BS, Jordan APO, Romano J, Leslie GJ, Alvarez X, O (2022 Jun). A cellular trafficking signal in the SIV envelope protein cytoplasmic domain is strongly selected for in pathogenic infection. PLoS pathogens, 18(6), e1010507. . ID: 35714165.
10.) Currey JM, Rabito F, Maness NJ, Blair RV, Rappaport J, Qin X, Kolls JK, Srivastava AK (2022 May 5). C57BL/6J Mice Are Not Suitable for Modeling Severe SARS-CoV-2 Beta and Gamma Variant Infection. Viruses, 14(5), . . ID: 35632708.
11.) Madden PJ, Thomas Y, Blair RV, Samer S, Doyle M, Midkiff CC, Doyle-Meyers LA, Becker ME, Arif MS, McRaven MD, Simons LM, Carias AM, Martinelli E, Lorenzo-Redondo R, Hultquist JF, Villinger FJ, Veazey RS, Hope TJ (2022 Apr 8). An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques. Research square, (), . . ID: 35411346.
12.) Rutkai I, Mayer MG, Hellmers LM, Ning B, Huang Z, Monjure CJ, Coyne C, Silvestri R, Golden N, Hensley K, Chandler K, Lehmicke G, Bix GJ, Maness NJ, Russell-Lodrigue K, Hu TY, Roy CJ, Blair RV, Bohm R, Doyle-Meyers LA, Rappaport J, Fischer T (2022 Apr 1). Neuropathology and virus in brain of SARS-CoV-2 infected non-human primates. Nature communications, 13(1), 1745. . ID: 35365631.
13.) Pillet S, Arunachalam PS, Andreani G, Golden N, Fontenot J, Aye PP, Röltgen K, Lehmicke G, Gobeil P, Dubé C, Trépanier S, Charland N, D (2022 Feb). Safety, immunogenicity, and protection provided by unadjuvanted and adjuvanted formulations of a recombinant plant-derived virus-like particle vaccine candidate for COVID-19 in nonhuman primates. Cellular & molecular immunology, 19(2), 222-233. . ID: 34983950.
14.) Melton A, Doyle-Meyers LA, Blair RV, Midkiff C, Melton HJ, Russell-Lodrigue K, Aye PP, Schiro F, Fahlberg M, Szeltner D, Spencer S, Beddingfield BJ, Goff K, Golden N, Penney T, Picou B, Hensley K, Chandler KE, Plante JA, Plante KS, Weaver SC, Roy CJ, Hoxie JA, Gao H, Montefiori DC, Mankowski JL, Bohm RP, Rappaport J, Maness NJ (2021 Dec). The pigtail macaque (Macaca nemestrina) model of COVID-19 reproduces diverse clinical outcomes and reveals new and complex signatures of disease. PLoS pathogens, 17(12), e1010162. . ID: 34929014.
15.) Beddingfield BJ, Maness NJ, Fears AC, Rappaport J, Aye PP, Russell-Lodrigue K, Doyle-Meyers LA, Blair RV, Carias AM, Madden PJ, Redondo RL, Gao H, Montefiori D, Hope TJ, Roy CJ (2021). Effective Prophylaxis of COVID-19 in Rhesus Macaques Using a Combination of Two Parenterally-Administered SARS-CoV-2 Neutralizing Antibodies. Frontiers in cellular and infection microbiology, 11(), 753444. . ID: 34869063.
16.) Madden PJ, Arif MS, Becker ME, McRaven MD, Carias AM, Lorenzo-Redondo R, Xiao S, Midkiff CC, Blair RV, Potter EL, Martin-Sancho L, Dodson A, Martinelli E, Todd JM, Villinger FJ, Chanda SK, Aye PP, Roy CJ, Roederer M, Lewis MG, Veazey RS, Hope TJ (2021). Development of an In Vivo Probe to Track SARS-CoV-2 Infection in Rhesus Macaques. Frontiers in immunology, 12(), 810047. . ID: 35003140.
The Confocal Microscopy and Molecular Pathology Core (CMMPC) provides research support and training with molecular pathology skills, confocal microscopy, image analysis, and multicolor immunohistochemistry and in-situ hybridization techniques for internal and affiliate scientists. Services include assistance with experimental design, antibody selection, molecular probe selection, fixation and staining protocols, operating fluorescent and confocal microscopes, acquiring and storing imaging data, interpretation of results, and generation of publication-quality images. Image analysis services include assistance with capturing images for evaluation and with use of various image analysis software.
