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TNPRC Pathogen Detection and Quantification Core

Tulane University

Contact Info:

Carolina Allers, PhD/Mary Barnes

Tulane National Primate Research Center

18703 Three Rivers Road

Covington, LA 70433-8915

https://tnprc.tulane.edu/pathogen-detection-and-quantification-core

Grants and Identifiers:

RRID: RRID:SCR_024614

Instrumentation:

Bio-Plex® 200 Suspension Array System

The Bio-Plex® 200 system is a suspension array reader that provides a reliable multiplex assay solution permiting analysis of up to 100 biomolecules in a single sample. [Product Link]

RRID:SCR_018026

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BioTek Synergy LX Multi-Mode Microplate Reader

Microplate reader with an optic design that provides accurate data in absorbance, fluorescence and luminescence detection modes, enabling many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford and cell viability. [Product Link]

RRID:SCR_019763

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Life Technologies QuantStudio 6 Real Time PCR System

QuantStudio 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well block in this case); other block types may be purchased separately. For a system that also supports TaqMan array cards, as well as automation, please see our QuantStudio 7 Flex system. The QuantStudio 6 Flex system can also be upgraded to a QuantStudio 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio 12K Flex system, which also accommodates TaqMan OpenArray plates. [Product Link]

RRID:SCR_020239

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(Equipment No Longer Active At this Facility)

Life Technologies QuantStudio 7 Real Time PCR System

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, software, automation capabilities, and touch-screen interface, the QuantStudio 7 Flex system offers reproducibility with minimal well-to-well and instrument-to-instrument variation. [Product Link]

RRID:SCR_020245

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Roche MagNA Pure 96 System

High-throughput robotic workstation for fully automated purification of nucleic acids from up to 96 samples.

Thermo Fisher Scientific QuantStudio 12K Flex

Services Provided:

Blood Processing

CDNA Synthesis

Cytokine Assays

DNA Isolation

ELISA

Immunoassay

Luminex Bead Based Cytokine Assays

Luminex Services

Nucleic Acid Extraction

OpenArray

Plate Reader

Real-time qPCR

RNA Isolation

Relevant Publications:

1.) Kaur A, Manuel T, Moström M, Crooks C, Davalos A, Barfield R, Scheef E, Kendall S, Midkiff C, Sprehe L, Trexler M, Boquet F, Shroyer M, Danner V, Doyle-Meyers L, Weinbaum C, Mirza A, Lammi S, Otero C, Lee M, Rogers L, Granek J, Owzar K, Malouli D, Fruh K, Kowalik T, Chan C, Permar S, Blair R (2025 Apr 23). Nonhuman primate model mirroring human congenital cytomegalovirus infection reveals a spectrum of vertical transmission outcomes Research Square, (), rs.3.rs-6378923. . PMCID: 12045369.


2.) Bohannon DG, Zablocki-Thomas LD, Leung ES, Dupont JK, Hattler JB, Kowalewska J, Zhao M, Luo J, Salemi M, Amedee AM, Li Q, Kuroda MJ, Kim WK (2024 Jul 25). CSF1R inhibition depletes brain macrophages and reduces brain virus burden in SIV-infected macaques Brain, 147(9), 3059-3069. . PMCID: 11370798.


3.) Thirugnanam S, Wang C, Zheng C, Grasperge BF, Datta PK, Rappaport J, Qin X, Rout N (2024 Jun 17). High IL-1β and IL-18 Levels Associate with Gut Barrier Disruption and Monocyte Activation During Chronic SIV Infection with Long-Term Suppressive Antiretroviral Therapy. bioRxiv : the preprint server for biology, (), . . PMID: 38948748.


4.) Zablocki-Thomas L, Ardeshir A, Takahashi N, White KS, Sumer CE, Wallis ZK, Didier ES, Kim WK, Williams KC, Kuroda MJ (2024 Jan 2). Plasma galectin-9 levels correlate with blood monocyte turnover and predict simian/human immunodeficiency virus disease progression Translational medicine communications, 9(1), 1. . PMCID: 11737433.


5.) Parthasarathy G (2024). Fibroblast growth factor receptor inhibitors mitigate the neuropathogenicity of Borrelia burgdorferi or its remnants ex vivo. Frontiers in immunology, 15(), 1327416. . PMID: 38638441.


6.) Melton A, Rowe LA, Penney T, Krzykwa C, Goff K, Scheuermann S, Melton HJ, Williams K, Golden N, Green KM, Smith B, Russell-Lodrigue K, Dufour JP, Doyle-Meyers LA, Schiro F, Aye PP, Lifson JD, Beddingfield BJ, Blair RV, Bohm RP, Kolls JK, Rappaport J, Hoxie JA, Maness NJ (2023 Nov 16). The Impact of SIV-Induced Immunodeficiency on Clinical Manifestation, Immune Response, and Viral Dynamics in SARS-CoV-2 Coinfection. bioRxiv : the preprint server for biology, (), . . PMID: 38014096.


7.) Blair RV, Vaccari M, Doyle-Meyers LA, Roy CJ, Russell-Lodrigue K, Fahlberg M, Monjure CJ, Beddingfield B, Plante KS, Plante JA, Weaver SC, Qin X, Midkiff CC, Lehmicke G, Golden N, Threeton B, Penney T, Allers C, Barnes MB, Pattison M, Datta PK, Maness NJ, Birnbaum A, Fischer T, Bohm RP, Rappaport J (2021 Feb). Acute Respiratory Distress in Aged, SARS-CoV-2-Infected African Green Monkeys but Not Rhesus Macaques. The American journal of pathology, 191(2), 274-282. . ID: 33171111.


8.) Monjure CJ, Tatum CD, Panganiban AT, Arainga M, Traina-Dorge V, Marx PA Jr, Didier ES (2014 Feb). Optimization of PCR for quantification of simian immunodeficiency virus genomic RNA in plasma of rhesus macaques (Macaca mulatta) using armored RNA. Journal of medical primatology, 43(1), 31-43. . ID: 24266615.


Description:

The Pathogen Detection and Quantification Core (PDQC) provides services for maintaining the SPF (Specific Pathogen Free) status in macaque breeding colonies. Services include antibody detection in serum or plasma by Luminex-based assays and /or ELISA for SPF-agents like SIV, SRV, STLV, B virus, SFV, CMV, RRV, SVV, SV40, and LCV; and non-SPF infectious agents like measles, Burkholderia pseudomallei, Tripanosoma cruzi, Mycobacterium tuberculosis, and SARS-CoV-2. The PDQC also offers services of cytokines quantification, and molecular assays for detection of SRV, CMV, SFV, and RRV. In addition, the PDQC performs nucleic acid purification from blood and tissue samples as well as qPRC for SIV viral load in plasma, or cells.