Pennsylvania State University

Contact Info:

Cheryl Keller

https://www.huck.psu.edu/about/collaborative-ventures/genomics-research-incubator/about-the-genomics-research-incubator

Grants and Identifiers:

RRID: RRID:SCR_024530

Instrumentation:

Services Provided:

Relevant Publications:

1.) Kulkarni S, Morrissey A, Sebastian A, Akinniyi OT, Keller CA, Albert I, Mahony S, Reese JC (2026 Jan 17). Human CCR4-NOT suppresses pervasive transcription and retrotransposable elements. bioRxiv : the preprint server for biology, (), . . PMCID: 12871343.

2.) Akinniyi OT, Sebastian A, Kulkarni S, Albert I, Reese. JC (2025 Dec 12). Degradation factor 1, Def1, regulates mRNA translation and decay through Ccr4-Not-dependent ubiquitylation of the ribosome bioRxiv, (), 2025.12.10.693450. . PMCID: 12710963.

3.) Akinniyi OT, Kulkarni S, Hribal MM, Keller CA, Giardine BM, Reese JC (2025 Dec 5). Gene expression analysis and proximity labeling reveal post-transcriptional functions of the yeast RNA polymerase II regulator Def1. The Journal of biological chemistry, 302(2), 111003. . PMCID: 12804109.

4.) Koo H, Heber K, Tian S, Connolly ST, Hao F, Zhao J, Swencki-Underwood B, Patterson AD, Townsend GE, Bisanz JE (2025 Nov 12). A synthetic gut microbiota provides an understanding of the maintenance and functional impact of phage mBio, 16(12), e02341-25. . PMCID: 12691588.

5.) James DQ, Mukherjee S, Cannon CC, Mahony S (2025 Nov 7). Optimized ChIP-exo for mammalian cells and patterned sequencing flow cells G3: Genes | Genomes | Genetics, 16(1), jkaf270. . PMCID: 12774610.

6.) Kulkarni S, Smith C, Akinniyi OT, Giardine BM, Keller CA, Arnaoutov A, Reese JC (2025 Oct 27). Auxin-induced depletion of human CCR4-NOT subunits reveals opposing functions of CNOT1 and CNOT4 in mRNA metabolism The Journal of Biological Chemistry, 301(12), 110862. . PMCID: 12682128.

7.) Wang X, Aboreden NG, Cai Y, Lam JC, Henderson KA, Xiang J, Giardine BM, Hardison RC, Keller CA, Nagarajan L, Brandt SJ, Blobel GA (2025 Aug 21). Single-stranded DNA-binding proteins are essential components of the architectural LDB1 protein complex. Molecular cell, 85(16), 3074-3089.e8. . PMID: 40803327.

8.) Akinniyi OT, Sebastian A, Kulkarni S, Hribal MM, Keller CA, Giardine BM, Albert I, Reese JC (2025 Apr 8). The DNA damage response and RNA Polymerase II regulator Def1 has posttranscriptional functions in the cytoplasm. bioRxiv : the preprint server for biology, (), . . PMID: 39463967.

9.) Kulkarni S, Morrissey A, Sebastian A, Giardine B, Smith C, Akinniyi OT, Keller CA, Arnaoutov A, Albert I, Mahony S, Reese JC (2024 Nov 25). Human CCR4-NOT globally regulates gene expression and is a novel silencer of retrotransposon activation. bioRxiv : the preprint server for biology, (), . . PMID: 39314347.

Description:

Provides access to highly specialized and custom genomics and epigenomics assays that Genomics Core Facility does not currently support. Examples include ATAC sequencing; RUN sequencing;Tag sequencing;Chromatin immunoprecipitation;Custom assays. Offers hands-on experiences and training for researchers in genomic technologies, methodologies, and instrumentation.