Pennsylvania State University
Sung Hyun (Joseph) Cho
W231 Millennium Science Complex
University Park, PA 16802
https://www.huck.psu.edu/core-facilities/cryo-electron-microscopy-facility
RRID: RRID:SCR_024456
Gatan K2 Direct Electron Detector
PELCO easiGlow Glow Discharge Cleaning System
Thermo Fisher Scientific Falcon 4 Direct Electron Detector
Thermo Fisher Scientific Image Corrector
Thermo Fisher Scientific Krios G3 Cryo-TEM
Thermo Fisher Scientific Talos Arctica Cryo-TEM
Thermo Fisher Scientific Vitrobot Mark IV
Cryo-Electron Microscopy
Cryo-Electron Microscopy Of Vitrified Sections (CEMOVIS)
Cryo-Electron Tomography
Data Analysis
Data Analysis And Interpretation
Data Processing
Data Processing And Interpretation
Data Visualization
Electron Microscopy
End-to-end Service
High Performance Computing
Image Aquisition
Image Processing And Analysis
Negative Staining For TEM
Training
Transmission Electron Microscopy
Vitrification
1.) Adu OF, Lee H, Früh SP, Schoenle MV, Weichert WS, Flyak AI, Hafenstein SL, Parrish CR (2025 Feb 14). Structures and functions of the limited natural polyclonal antibody response to parvovirus infection Proceedings of the National Academy of Sciences of the United States of America, 122(8), e2423460122. . PMCID: 11873831.
2.) Lee H, Assaraf R, Subramanian S, Goetschius D, Bieri J, DiNunno NM, Leisi R, Bator CM, Hafenstein SL, Ros C (2024 Nov 5). Infectious parvovirus B19 circulates in the blood coated with active host protease inhibitors Nature Communications, 15(), 9543. . PMCID: 11538491.
3.) Qayyum MZ, Imashimizu M, Leanca M, Vishwakarma RK, Riaz-Bradley A, Yuzenkova Y, Murakami KS (2024 Feb 14). Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase Proceedings of the National Academy of Sciences of the United States of America, 121(8), e2311480121. . PMCID: 10895346.
Cryo-Electron Microscopy Facility houses FEI Titan Krios microscope that offers data collection for life sciences while incorporating materials science applications. Used for creating super high definition 3D images of atoms and molecules. Facility allows for fully automated atomic resolution single particle and high contrast tomography tilt-series data collection. Additional microscopy components permit a full range of materials science applications, including EELS, STEM, and DPC. Facility also houses ThermoFisher Arctica G2.
